| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 180,446 | C→T | 50.0% | R217R (CGC→CGT) | lpxD → | UDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 180,446 | 0 | C | T | 50.0% | ‑1.6 / 116.1 | 124 | R217R (CGC→CGT) | lpxD | UDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (48/14); new base T (43/19); total (91/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 124 total aligned reads displayed.
CAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGTGCCA > minE/180384‑180502 | caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:1738860/1‑69 (MQ=255)caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:1732922/1‑69 (MQ=255)caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:1657149/1‑69 (MQ=255)caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:1138724/1‑69 (MQ=255)caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:3169239/1‑69 (MQ=255)caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:1373290/1‑69 (MQ=255)caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:2579199/1‑69 (MQ=255)caGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGgcgcg > 1:3037051/1‑69 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:2527436/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:2122508/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:1584035/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:312897/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:3413588/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:1839386/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:1980159/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:2902292/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:912677/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:1067336/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:2803262/1‑68 (MQ=255) tGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTgg > 1:1050360/1‑68 (MQ=255) ggTCGCGTAATTATTGGCTATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGAt < 1:602972/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:3324218/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:74881/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:583515/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:2016581/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:1729444/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:61100/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:3054206/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:2967225/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:829731/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:1141003/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:2820240/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGAt < 1:934513/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGAt < 1:842352/69‑1 (MQ=255) ggTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGAt < 1:78168/69‑1 (MQ=255) gTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTACCATTGATCGTGGGGCGCTGGAt < 1:76438/68‑1 (MQ=255) cgcgTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGa < 1:3205525/68‑1 (MQ=255) gcgTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGa < 1:2308309/67‑1 (MQ=255) ttGGCGATCGCGTGGAGATCGGCGCCTGCACAACCATCGATCGCGGCGCGCTGGATGac < 1:2772694/59‑1 (MQ=255) ggCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGc < 1:2234200/69‑1 (MQ=255) ggCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGc < 1:2800110/69‑1 (MQ=255) ggCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGc < 1:786463/69‑1 (MQ=255) ggCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGc < 1:1025820/69‑1 (MQ=255) gagaTCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:929586/42‑1 (MQ=37) gaTCGGTGCCTGCACTACCATTGATCGTGGCGCGCTGGAt < 1:1435639/40‑1 (MQ=37) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2823306/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2755024/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2683941/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2683337/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2675227/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2636360/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2949576/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2951782/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2417533/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2410818/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:1455111/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2303557/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:2299700/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:676600/1‑68 (MQ=255) cGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTg > 1:412546/1‑68 (MQ=255) gCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAAc < 1:2673467/69‑1 (MQ=255) gCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAAc < 1:1550227/69‑1 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:1041004/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:3373814/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:3259472/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:725867/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:3377935/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:2066593/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:1353266/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:1210569/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGt > 1:894159/1‑68 (MQ=255) cacaACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCAATCATAAcc > 1:1861576/1‑65 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGcc > 1:149443/1‑65 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:1627420/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:629185/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:1718477/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:1837784/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:179584/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:1502549/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:146234/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:1395101/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:112489/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:1056004/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:1982873/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:2916773/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:2928299/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:2473964/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:2441298/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:2408723/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:2272087/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:221/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:2130200/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:1831702/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:3226702/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:3244311/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:3272141/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:2728897/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:374468/1‑66 (MQ=255) cATTGATCGTGGCGCGCTGGATGACACTGTTATTGG‑AATGGTGTGATCATTGATAACCAGTGCCa > 1:3381105/1‑65 (MQ=255) cATTGATCGTGGCGCGATGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCa > 1:3435800/1‑66 (MQ=255) | CAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTGGATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGTGCCA > minE/180384‑180502 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |