Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,130,221 A→G 15.9% C228C (TGT→TGC)  mtr ← tryptophan transporter of high affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,130,2210AG15.9% 174.1 / 19.4 106C228C (TGT→TGC) mtrtryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base A (56/33);  new base G (4/13);  total (60/46)
Fisher's exact test for biased strand distribution p-value = 3.38e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 106 total aligned reads displayed.

GTTACCCATCGTCGCCAGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGTG  >  minE/2130155‑2130283
                                                                  |                                                              
gTTACCCATCGTCGCCAGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACa                                                              <  1:2008042/69‑1 (MQ=255)
   aCCCATCGTCGCCAGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACAttt                                                           >  1:198934/1‑69 (MQ=255)
   aCCCATCGTCGCCAGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACAttt                                                           >  1:1929783/1‑69 (MQ=255)
          gtcgCCAGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATg                                                    >  1:3107680/1‑69 (MQ=255)
          gtcgCCAGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATg                                                    >  1:3149050/1‑69 (MQ=255)
          gtcgCCAGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATg                                                    >  1:3150728/1‑69 (MQ=255)
          gtcgCCAGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATg                                                    >  1:3201756/1‑69 (MQ=255)
          gtcgCCAGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATg                                                    >  1:2676400/1‑69 (MQ=255)
          gtcgCCAGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATg                                                    >  1:2626567/1‑69 (MQ=255)
          gtcgCCAGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATg                                                    >  1:2609312/1‑69 (MQ=255)
          gtcgCCAGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATg                                                    >  1:3268656/1‑69 (MQ=255)
          gtcgCCAGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATg                                                    >  1:387415/1‑69 (MQ=255)
          gtcgCCAGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATg                                                    >  1:1274878/1‑69 (MQ=255)
                aGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTc                                                          <  1:605608/57‑1 (MQ=255)
                aGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTc                                                          <  1:702555/57‑1 (MQ=255)
                aGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTc                                                          <  1:1093705/57‑1 (MQ=255)
                aGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTc                                                          <  1:2599698/57‑1 (MQ=255)
                aGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTc                                                          <  1:3246023/57‑1 (MQ=255)
                aGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTc                                                          <  1:3374061/57‑1 (MQ=255)
                aGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTc                                                          <  1:3375344/57‑1 (MQ=255)
                aGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTc                                                          <  1:3406787/57‑1 (MQ=255)
                aGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTc                                                          <  1:2165311/57‑1 (MQ=255)
                aGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTc                                                          <  1:3416627/57‑1 (MQ=255)
                aGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTc                                                          <  1:2829280/57‑1 (MQ=255)
                aGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTc                                                          <  1:1814641/57‑1 (MQ=255)
                aGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTc                                                          <  1:347299/57‑1 (MQ=255)
                aGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTc                                                          <  1:3163978/57‑1 (MQ=255)
                aGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTc                                                          <  1:470018/57‑1 (MQ=255)
                aGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTc                                                          <  1:3035264/57‑1 (MQ=255)
                       aGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACGATGGTTTTCGGATCtt                                       >  1:1055069/1‑69 (MQ=255)
                       aGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACGATGGTTTTCGGATCtt                                       >  1:2085194/1‑69 (MQ=255)
                       aGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACGATGGTTTTCGGATCt                                        >  1:1172761/1‑68 (MQ=255)
                       aGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCtt                                       <  1:3109494/69‑1 (MQ=255)
                       aGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCtt                                       <  1:2013378/69‑1 (MQ=255)
                       aGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCtt                                       <  1:324095/69‑1 (MQ=255)
                       aGATGGGATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACGATGGTTTTCGGATCtt                                       >  1:437524/1‑69 (MQ=255)
                              ataCAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGt                                 >  1:1734718/1‑68 (MQ=255)
                              ataCAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGt                                 >  1:569661/1‑68 (MQ=255)
                              ataCAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGt                                 >  1:1463904/1‑68 (MQ=255)
                              ataCAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGt                                 >  1:3298392/1‑68 (MQ=255)
                              ataCAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGt                                 >  1:531961/1‑68 (MQ=255)
                              ataCAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGt                                 >  1:2173079/1‑68 (MQ=255)
                              ataCAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGt                                 >  1:1882711/1‑68 (MQ=255)
                               taCAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACTATGGTTTTCGGATCTTTGCCGTaa                               <  1:358446/69‑1 (MQ=255)
                               taCAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACGATGGTTTTCGGATCTTTGCCGTaa                               <  1:2753006/69‑1 (MQ=255)
                               taCAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACGATGGTTTTCGGATCTTTGCCGTaa                               <  1:3009938/69‑1 (MQ=255)
                               taCAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACGATGGTTTTCGGATCTTTGCCGTaa                               <  1:1266718/69‑1 (MQ=255)
                               taCAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACGATGGTTTTCGGATCTTTGCCGTaa                               <  1:1070624/69‑1 (MQ=255)
                               taCAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACGATGGTTTTCGGATCTTTGCCGTaa                               <  1:1166353/69‑1 (MQ=255)
                               taCAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACGATGGTTTTCGGATCTTTGCCGTaa                               <  1:3311532/69‑1 (MQ=255)
                               taCAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACGATGGTTTTCGGATCTTTGCCGTaa                               <  1:654948/69‑1 (MQ=255)
                               taCAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACGATGGTTTTCGGATCTTTGCCGTaa                               <  1:1188930/69‑1 (MQ=255)
                               taCAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACGATGGTTTTCGGATCTTTGCCGTaa                               <  1:1675154/69‑1 (MQ=255)
                               taCAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACGATGGTTTTCGGATCTTTGCCGTaa                               <  1:645497/69‑1 (MQ=255)
                               taCAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACGATGGTTTTCGGATCTTTGCCGTaa                               <  1:548570/69‑1 (MQ=255)
                               taCAGCGCCAGCGCCATCAGCGTACCGTACACCAGGCATTTCACGATGGTTTTCGGATCTTTGCCGTaa                               <  1:1387776/69‑1 (MQ=255)
                                 cagcgccagcgcCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGt                                 <  1:3215718/65‑1 (MQ=255)
                                 cagcgccagcgcCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGt                                 <  1:914350/65‑1 (MQ=255)
                                 cagcgccagcgcCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGt                                 <  1:918395/65‑1 (MQ=255)
                                 cagcgccagcgcCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGt                                 <  1:1017563/65‑1 (MQ=255)
                                 cagcgccagcgcCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGt                                 <  1:2979894/65‑1 (MQ=255)
                                 cagcgccagcgcCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTaa                               <  1:2571574/67‑1 (MQ=255)
                                 cagcgccagcgcCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAAt                              >  1:2985759/1‑68 (MQ=255)
                                 cagcgccagcgcCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAAt                              >  1:1494151/1‑68 (MQ=255)
                                 cagcgccagcgcCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAAt                              >  1:3270242/1‑68 (MQ=255)
                                      ccagcgccaTCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTtc                         <  1:2758455/68‑1 (MQ=255)
                                       cagctccaTCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGt                                 <  1:1213619/59‑1 (MQ=255)
                                                 aGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTaa                               >  1:1479799/1‑51 (MQ=255)
                                                 aGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTgg              >  1:59387/1‑68 (MQ=255)
                                                 aGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGc             >  1:1665761/1‑69 (MQ=255)
                                                 aGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGc             >  1:150443/1‑69 (MQ=255)
                                                 aGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGc             >  1:50445/1‑69 (MQ=255)
                                                 aGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGc             >  1:519203/1‑69 (MQ=255)
                                                 aGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGc             >  1:632320/1‑69 (MQ=255)
                                                 aGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGc             >  1:1055761/1‑69 (MQ=255)
                                                 aGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGc             >  1:807709/1‑69 (MQ=255)
                                                 aGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGc             >  1:1000508/1‑69 (MQ=255)
                                                 aGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGc             >  1:313313/1‑69 (MQ=255)
                                                 aGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGc             >  1:2944888/1‑69 (MQ=255)
                                                 aGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGc             >  1:2790120/1‑69 (MQ=255)
                                                 aGCGTACCGTACACCAGACATTTCACGATGGTTTTAGTATCTTTGCCGTAATACTTCATCAGGCTTGGc             >  1:1168873/1‑69 (MQ=255)
                                                 aGCGGACCGTACACCAGACATTTCACGATGGGTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGc             >  1:2233875/1‑69 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGt   >  1:1998239/1‑68 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGt   >  1:726368/1‑68 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGt   >  1:668740/1‑68 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGt   >  1:2563997/1‑68 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGt   >  1:2471440/1‑68 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGt   >  1:1475148/1‑68 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGt   >  1:2450744/1‑68 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGt   >  1:2431288/1‑68 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGt   >  1:2264854/1‑68 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGt   >  1:148761/1‑68 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGt   >  1:406731/1‑68 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGt   >  1:3054610/1‑68 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGt   >  1:362754/1‑68 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGt   >  1:1727629/1‑68 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGTg  <  1:1289359/69‑1 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGTg  <  1:1273945/69‑1 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGTg  <  1:2798872/69‑1 (MQ=255)
                                                            cacCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGTg  <  1:1025784/69‑1 (MQ=255)
                                                                  |                                                              
GTTACCCATCGTCGCCAGCAACCAGATGGTATACAGCGCCAGCGCCATCAGCGTACCGTACACCAGACATTTCACGATGGTTTTCGGATCTTTGCCGTAATACTTCATCAGGCTTGGCACGTTACCGTG  >  minE/2130155‑2130283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑