| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,130,401 | A→G | 41.5% | F168F (TTT→TTC) | mtr ← | tryptophan transporter of high affinity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,130,401 | 0 | A | G | 41.5% | 20.8 / 46.8 | 65 | F168F (TTT→TTC) | mtr | tryptophan transporter of high affinity |
| Reads supporting (aligned to +/- strand): ref base A (18/20); new base G (13/14); total (31/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.05e-01 | |||||||||||
TAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCGACTGACGGCTTT > minE/2130336‑2130466 | tAAGACTCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAg < 1:1033654/69‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGGACATGCCCCAGCAGGCTACCaaa < 1:93526/68‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:698360/68‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:2136373/68‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:714634/68‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:2335987/68‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:1885379/68‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:1793678/68‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:1639404/68‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:1632927/68‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:1626609/68‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:2572912/68‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:493918/68‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:1548390/68‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAg < 1:2653923/69‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAg < 1:2022004/69‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAg < 1:2021253/69‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAg < 1:1744405/69‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAg < 1:726449/69‑1 (MQ=255)tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAg < 1:887075/69‑1 (MQ=255)tAAGACGCATTACTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:2684097/68‑1 (MQ=255) aCGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTg < 1:2399777/68‑1 (MQ=255) aCGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTg < 1:468432/68‑1 (MQ=255) aCGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTg < 1:2887775/68‑1 (MQ=255) aCGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTg < 1:131530/68‑1 (MQ=255) aCGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTg < 1:294238/68‑1 (MQ=255) aCGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTg < 1:2534272/68‑1 (MQ=255) aCGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTg < 1:3040532/68‑1 (MQ=255) gCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGgaagaa > 1:339937/1‑69 (MQ=255) gCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGgaagaa > 1:863348/1‑69 (MQ=255) tgCGACGTTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:3594/2‑69 (MQ=255) tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCGGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:1379762/2‑69 (MQ=25) tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa > 1:2536464/2‑68 (MQ=37) tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa > 1:2290954/2‑68 (MQ=37) tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:2658812/2‑69 (MQ=37) tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:2283440/2‑69 (MQ=37) tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:456002/2‑69 (MQ=37) tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:1917685/2‑69 (MQ=37) tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:759783/2‑69 (MQ=37) tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:811204/2‑69 (MQ=37) tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:415445/2‑69 (MQ=37) tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:1526326/2‑69 (MQ=37) tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:1089204/2‑69 (MQ=37) ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:2968174/68‑1 (MQ=255) ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:37043/68‑1 (MQ=255) ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:2036500/68‑1 (MQ=255) ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:599399/68‑1 (MQ=255) ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:2223543/68‑1 (MQ=255) ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:2071513/68‑1 (MQ=255) ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:1905484/68‑1 (MQ=255) ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:189644/68‑1 (MQ=255) ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:85796/68‑1 (MQ=255) ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:1433594/68‑1 (MQ=255) ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:1389877/68‑1 (MQ=255) gCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:3429362/67‑1 (MQ=255) gCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGCGAGGAAGAAGGTaa < 1:1563873/67‑1 (MQ=255) attGTCGCAGGCT‑CACATGCCCCAGCAGGCTACCAAAGGTg < 1:3176815/39‑1 (MQ=38) gTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:1116691/52‑1 (MQ=255) gTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGgaaga > 1:954800/1‑46 (MQ=255) gTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGgaaga > 1:3230599/1‑46 (MQ=255) gTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGgaaga > 1:1443994/1‑46 (MQ=255) gTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGgaaga > 1:162899/1‑46 (MQ=255) gTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGgaag > 1:3381801/1‑45 (MQ=255) tGCCCCAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCAt > 1:1052299/1‑68 (MQ=255) tGCCCCAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCAt > 1:1551943/1‑68 (MQ=255) tGCCCCAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCAt > 1:549448/1‑68 (MQ=255) tGCCCCAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCAt > 1:264828/1‑68 (MQ=255) tGCCCCAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCAt > 1:2738631/1‑68 (MQ=255) tGCCCCAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCAt > 1:3229501/1‑68 (MQ=255) ccccAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCg > 1:1689992/1‑69 (MQ=255) ccccAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCg > 1:2878853/1‑69 (MQ=255) ccccAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCg > 1:871748/1‑69 (MQ=255) ccccAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCg > 1:1359046/1‑69 (MQ=255) ccccAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCg > 1:948228/1‑69 (MQ=255) aCCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCGACTGACGGCttt < 1:2573487/69‑1 (MQ=255) aCCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCGACTGACGGCttt < 1:2264185/69‑1 (MQ=255) aCCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCGACTGACGGCttt < 1:2072916/69‑1 (MQ=255) aCCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCGACTGACGGCttt < 1:1981538/69‑1 (MQ=255) aCCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCGACTGACGGCttt < 1:1223719/69‑1 (MQ=255) | TAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCGACTGACGGCTTT > minE/2130336‑2130466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |