Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,130,596 G→A 16.2% T103T (ACC→ACT mtr ← tryptophan transporter of high affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,130,5960GA16.2% 229.6 / 25.1 134T103T (ACC→ACTmtrtryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base G (45/67);  new base A (20/2);  total (65/69)
Fisher's exact test for biased strand distribution p-value = 9.90e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 134 total aligned reads displayed.

GTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCT  >  minE/2130533‑2130650
                                                               |                                                      
gTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAg                                                   <  1:532943/69‑1 (MQ=255)
gTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAg                                                   <  1:1792108/69‑1 (MQ=255)
gTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAg                                                   <  1:2281584/69‑1 (MQ=255)
gTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAg                                                   <  1:1600427/69‑1 (MQ=255)
gTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAg                                                   <  1:1570024/69‑1 (MQ=255)
gTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAg                                                   <  1:1434171/69‑1 (MQ=255)
gTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAg                                                   <  1:1203901/69‑1 (MQ=255)
 tttAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAg                                                   <  1:1489665/68‑1 (MQ=255)
           tctcTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAg                                                   <  1:2608884/58‑1 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:126116/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:2004961/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:2101960/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:1812188/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:3189031/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:3414930/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:2147367/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:2677715/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:1631414/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:320011/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:820279/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:898343/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:2661593/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:1532304/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:857300/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:2348143/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:1442867/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:1436378/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:2653557/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:1405944/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:2582367/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:127535/1‑67 (MQ=255)
             tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGACAGGATATAGAGca                                        >  1:127714/1‑67 (MQ=255)
             tctGCGAAGGTGAGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGca                                        >  1:1472793/1‑67 (MQ=255)
                       tgtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAAt                              >  1:2984141/1‑67 (MQ=255)
                       tgtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATg                             >  1:3206290/1‑68 (MQ=255)
                       tgtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATg                             >  1:2143503/1‑68 (MQ=255)
                       tgtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATg                             >  1:1220504/1‑68 (MQ=255)
                       tgtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATg                             >  1:306261/1‑68 (MQ=255)
                        gtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAg                                                   <  1:277749/45‑1 (MQ=255)
                        gtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAg                                                   <  1:1844827/45‑1 (MQ=255)
                        gtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:694390/69‑1 (MQ=255)
                        gtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:2839353/69‑1 (MQ=255)
                        gtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:872038/69‑1 (MQ=255)
                        gtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:2107635/69‑1 (MQ=255)
                        gtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:1127730/69‑1 (MQ=255)
                        gtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:3001106/69‑1 (MQ=255)
                        gtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:2269675/69‑1 (MQ=255)
                         tgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:3265861/68‑1 (MQ=255)
                           aTGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAAt                        >  1:696543/1‑69 (MQ=255)
                              cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:3359740/63‑1 (MQ=255)
                              cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:1496099/63‑1 (MQ=255)
                              cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:1493187/63‑1 (MQ=255)
                              cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:2741332/63‑1 (MQ=255)
                              cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGcc                     >  1:2575105/1‑69 (MQ=255)
                                  aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:1349687/59‑1 (MQ=255)
                                  aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:2382176/59‑1 (MQ=255)
                                  aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:2032942/59‑1 (MQ=255)
                                  aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:2367123/59‑1 (MQ=255)
                                  aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:1728988/59‑1 (MQ=255)
                                  aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:3191330/59‑1 (MQ=255)
                                  aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:1828574/59‑1 (MQ=255)
                                  aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:3033988/59‑1 (MQ=255)
                                  aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:3186995/59‑1 (MQ=255)
                                   tCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                               >  1:2882196/1‑54 (MQ=255)
                                   tCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                               >  1:3291913/1‑54 (MQ=255)
                                   tCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                               >  1:3385969/1‑54 (MQ=255)
                                   tCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                               >  1:1028534/1‑54 (MQ=255)
                                   tCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                               >  1:509551/1‑54 (MQ=255)
                                   tCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                               >  1:2699130/1‑54 (MQ=255)
                                   tCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                               >  1:2320456/1‑54 (MQ=255)
                                   tCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                               >  1:2204425/1‑54 (MQ=255)
                                   tCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                               >  1:2084245/1‑54 (MQ=255)
                                   tCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                               >  1:774548/1‑54 (MQ=255)
                                   tCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                               >  1:1808509/1‑54 (MQ=255)
                                   tCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                               >  1:1734841/1‑54 (MQ=255)
                                   tCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                               >  1:1622818/1‑54 (MQ=255)
                                   tCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                               >  1:1562216/1‑54 (MQ=255)
                                   tCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                               >  1:892307/1‑54 (MQ=255)
                                   tCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                               >  1:1099480/1‑54 (MQ=255)
                                   tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAgt                                  >  1:2060267/1‑50 (MQ=255)
                                   tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGc                                 >  1:596541/1‑52 (MQ=255)
                                   tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGc                                 >  1:1060112/1‑52 (MQ=255)
                                     gAACCACTGGCAGAAATATAGGGATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:935497/56‑1 (MQ=255)
                                     gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:1050354/56‑1 (MQ=255)
                                     gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:1125738/56‑1 (MQ=255)
                                     gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                           <  1:1405693/56‑1 (MQ=255)
                                         cccTGGAAGAAATATAGGCGTAAGTCAGGATATAGAGCACAAAGGCAATgg                            <  1:238522/49‑1 (MQ=255)
                                               cAGAAATATAGGCGTAAGTCAGGATATAGAGCACAAAGGCAATgg                            <  1:2416999/45‑1 (MQ=255)
                                                aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGcc   <  1:2007465/69‑1 (MQ=255)
                                                 gAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGcc   <  1:516881/68‑1 (MQ=255)
                                                 gAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt  <  1:2047358/69‑1 (MQ=255)
                                                 gAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt  <  1:1191015/69‑1 (MQ=255)
                                                 gAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt  <  1:1523606/69‑1 (MQ=255)
                                                 gAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt  <  1:826686/69‑1 (MQ=255)
                                                 gAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt  <  1:2730585/69‑1 (MQ=255)
                                                 gAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt  <  1:27444/69‑1 (MQ=255)
                                                 gAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt  <  1:380114/69‑1 (MQ=255)
                                                 gAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt  <  1:2850068/69‑1 (MQ=255)
                                                  aaaTATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt  <  1:1615390/68‑1 (MQ=255)
                                                    atatAGGCATAGGTCAGGATATAGACCACAAAGGCAATGGa                           <  1:3336410/41‑1 (MQ=255)
                                                               |                                                      
GTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCT  >  minE/2130533‑2130650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: