| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,131,478 | G→A | 39.2% | R490R (CGC→CGT) | deaD ← | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,131,478 | 0 | G | A | 39.2% | 105.8 / 131.7 | 191 | R490R (CGC→CGT) | deaD | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (64/52); new base A (69/6); total (133/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.97e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01 | |||||||||||
Only 100 of 191 total aligned reads displayed.
AGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCA > minE/2131421‑2131541 | aGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:1933508/69‑1 (MQ=255) aaTCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCat < 1:1030004/69‑1 (MQ=255) aaTCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCat < 1:627678/69‑1 (MQ=255) aaTCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCat < 1:823499/69‑1 (MQ=255) aaTCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCat < 1:1251901/69‑1 (MQ=255) aaTCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCat < 1:2845733/69‑1 (MQ=255) cGCACCAACTATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:297586/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCTGTACAGCTGc > 1:1873248/1‑64 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:206963/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:1991167/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:60737/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:2538727/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:2644994/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:1579232/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:873008/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:370793/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:130068/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:1214798/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:1387703/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCa > 1:820640/1‑65 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCa > 1:1657510/1‑65 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCa > 1:1715529/1‑65 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCa > 1:1920409/1‑65 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCa > 1:264960/1‑65 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACACCTGCa > 1:836776/1‑65 (MQ=255) aCGAACTTCAACACCATCATCGCGGGCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCacgacg > 1:440524/1‑69 (MQ=255) aCGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTa > 1:2516407/1‑42 (MQ=255) aCGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCacgacg > 1:1883825/1‑69 (MQ=255) aCGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCacgacg > 1:1769030/1‑69 (MQ=255) aCGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCacgacg > 1:1350797/1‑69 (MQ=255) aCGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCacgacg > 1:1554562/1‑69 (MQ=255) aCGAACTTCAACACCATCATCGCGGCCCACTTCAACGCGGTACAGCTGCATATCGCCAACATCacgacg > 1:3129516/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGtt > 1:1351659/1‑63 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTTacgac > 1:1727986/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacgac > 1:3123884/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacgac > 1:3402952/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacgac > 1:40753/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacgac > 1:716977/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacgac > 1:210461/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacgac > 1:1741076/1‑69 (MQ=255) atcatcGCTGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg < 1:503813/69‑1 (MQ=255) atcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg > 1:1058196/1‑69 (MQ=255) atcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg > 1:2759492/1‑69 (MQ=255) atcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg > 1:1534938/1‑69 (MQ=255) atcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg < 1:1842330/69‑1 (MQ=255) atcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg > 1:1184202/1‑69 (MQ=255) atcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg > 1:1093114/1‑69 (MQ=255) atcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg < 1:1081134/69‑1 (MQ=255) ctcGCGGCCCACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATc < 1:1885511/68‑1 (MQ=255) atcGCGTCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATc > 1:2328508/1‑69 (MQ=255) atcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATc > 1:3323398/1‑69 (MQ=255) atcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATc > 1:3371239/1‑69 (MQ=255) atcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATc > 1:2060158/1‑69 (MQ=255) atcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATc > 1:549280/1‑69 (MQ=255) atcGCGGCCCACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATc < 1:666641/69‑1 (MQ=255) atcGCGGCCCACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATc < 1:898334/69‑1 (MQ=255) atcGCGGCCCACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATc < 1:2878666/69‑1 (MQ=255) cgGCCCACTTCAATGCGGTAGAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGAct < 1:2152240/62‑2 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCa > 1:2183507/1‑64 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGt > 1:469296/1‑68 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:1293590/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:41779/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:2229389/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:447795/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:3138084/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:2015659/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:2723583/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:1454323/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:85258/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:1793513/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:583418/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:588052/1‑69 (MQ=255) ctacAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:2184122/4‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGtt > 1:3083840/1‑42 (MQ=38) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGtt > 1:2589087/1‑42 (MQ=38) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCgcga > 1:2456984/1‑47 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:650234/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:674993/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:2447696/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:392703/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1136503/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1811332/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1914801/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1981186/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:2064554/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:2196810/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:2256770/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:2348867/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:2963171/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:3065796/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:434587/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCt > 1:1380731/1‑59 (MQ=255) cTTCAATACGGTACAACTGAATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:291482/1‑60 (MQ=255) cTTCAATACGGTAAAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1011348/1‑60 (MQ=255) tCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGAcgcg < 1:237188/49‑1 (MQ=255) tCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGAcgcg < 1:2290504/49‑1 (MQ=255) tCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGAcgcg < 1:2695770/49‑1 (MQ=255) tCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGAcgcg < 1:2790212/49‑1 (MQ=255) tCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGAcgcg < 1:3242206/49‑1 (MQ=255) tCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGAcgcg < 1:943468/49‑1 (MQ=255) | AGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCA > minE/2131421‑2131541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |