| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,131,486 | G→A | 42.4% | L488L (CTG→TTG) | deaD ← | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,131,486 | 0 | G | A | 42.4% | 91.4 / 178.4 | 234 | L488L (CTG→TTG) | deaD | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (82/52); new base A (93/6); total (176/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.17e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 234 total aligned reads displayed.
AGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGCCAC > minE/2131421‑2131551 | aaTCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCat < 1:823499/69‑1 (MQ=255) aaTCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCat < 1:1251901/69‑1 (MQ=255) aaTCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCat < 1:42121/69‑1 (MQ=255) aTCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCat < 1:1887842/68‑1 (MQ=255) cGCACCAACTATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:297586/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:370793/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:1074259/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:1579232/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:1214798/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:1280393/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:130068/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:2538727/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:669827/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTg < 1:1387703/63‑1 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCa > 1:1715529/1‑65 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCa > 1:1657510/1‑65 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCa > 1:264960/1‑65 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCa > 1:137105/1‑65 (MQ=255) cGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACACCTGCa > 1:836776/1‑65 (MQ=255) aCGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCacgacg > 1:2068233/1‑69 (MQ=255) aCGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCacgacg > 1:2060457/1‑69 (MQ=255) aCGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCacgacg > 1:1554562/1‑69 (MQ=255) aCGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCacgacg > 1:445380/1‑69 (MQ=255) aCGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCacgacg > 1:1350797/1‑69 (MQ=255) aCGAACTTCAACACCATCATCGCGGCCCACTTCAACGCGGTACAGCTGCATATCGCCAACATCacgacg > 1:3129516/1‑69 (MQ=255) ttCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCacgacgg > 1:3063120/1‑63 (MQ=255) ttCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacg > 1:1182958/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTTacgac > 1:1727986/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCaggac > 1:462714/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacgac > 1:1741076/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacgac > 1:40753/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacgac > 1:3402952/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacgac > 1:916497/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacga > 1:1030802/1‑68 (MQ=255) atcatcGCTGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg < 1:503813/69‑1 (MQ=255) atcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg > 1:1534938/1‑69 (MQ=255) atcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg > 1:2759492/1‑69 (MQ=255) atcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg > 1:1880660/1‑69 (MQ=255) atcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg > 1:1093114/1‑69 (MQ=255) atcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg < 1:2002510/69‑1 (MQ=255) tcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg < 1:2853067/68‑1 (MQ=255) atcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATc > 1:2124240/1‑69 (MQ=255) atcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATc > 1:3323398/1‑69 (MQ=255) atcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATc > 1:3371239/1‑69 (MQ=255) atcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATc > 1:549280/1‑69 (MQ=255) atcGCGGCCCACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATc < 1:2878666/69‑1 (MQ=255) atcGCGGCCCACTTCAAAACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATc < 1:1885124/69‑1 (MQ=255) cgGCCCACTTCAATGCGGTAGAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGAct < 1:2152240/62‑2 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCa > 1:2183507/1‑64 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:583418/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:85258/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:2015659/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:347671/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:1805060/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:622497/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:775405/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:3138084/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:1833776/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:1849890/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:882772/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:2723583/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:2229389/1‑69 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGtt > 1:2589087/1‑42 (MQ=38) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGtt > 1:3083840/1‑42 (MQ=38) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCgcga > 1:2456984/1‑47 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGCCGCGGACGATCtt > 1:1487737/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:3205325/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:2256770/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:2196810/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:2363160/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:2447696/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:3291242/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:650234/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:677335/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1136503/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1919029/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:2963171/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:207982/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1932448/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1981186/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCt > 1:1380731/1‑59 (MQ=255) cTTCAATACGGTAAAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1011348/1‑60 (MQ=255) tCAATGCGGTACAGCTGCATATCGCCGACATCACGACGTTCACGACGCGGACGATCTTCACGGTcacca > 1:232237/1‑69 (MQ=255) tCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGAcgcg < 1:2790212/49‑1 (MQ=255) tCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGAcgcg < 1:597760/49‑1 (MQ=255) tCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGAcgcg < 1:2290504/49‑1 (MQ=255) tCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGAcgcg < 1:438318/49‑1 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:1689741/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:2336374/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:1355634/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:2191625/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:3090843/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:2945428/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:2625988/4‑69 (MQ=255) gTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCAcgcg > 1:2517703/1‑65 (MQ=255) gTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGcc > 1:392903/1‑69 (MQ=255) gTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGcc > 1:1773665/1‑69 (MQ=255) gTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGcc > 1:2462906/1‑69 (MQ=255) gTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGcc > 1:2689184/1‑69 (MQ=255) aCAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGCCAc > 1:2842719/1‑69 (MQ=255) | AGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGCCAC > minE/2131421‑2131551 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |