| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,131,514 | A→G | 54.8% | R478R (CGT→CGC) | deaD ← | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,131,514 | 0 | A | G | 54.8% | 49.1 / 167.6 | 201 | R478R (CGT→CGC) | deaD | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base A (68/23); new base G (89/21); total (157/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 | |||||||||||
Only 100 of 225 total aligned reads displayed.
TTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGCCACGGTCGTTACGATCGCGCGGACCACGGTCAT > minE/2131448‑2131581 | ttCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacg > 1:402232/1‑69 (MQ=255)ttCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacg > 1:1182958/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacgac > 1:1717531/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacgac > 1:1741076/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacgac > 1:1965054/1‑69 (MQ=255) cAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCacgac > 1:3123884/1‑69 (MQ=255) atcatcGCTGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg < 1:503813/69‑1 (MQ=255) atcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg > 1:2954214/1‑69 (MQ=255) atcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg > 1:2759492/1‑69 (MQ=255) atcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg < 1:1081731/69‑1 (MQ=255) atcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg > 1:1058196/1‑69 (MQ=255) tcatcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACg < 1:2853067/68‑1 (MQ=255) atcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATc > 1:3323398/1‑69 (MQ=255) atcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATc > 1:1384158/1‑69 (MQ=255) atcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATc > 1:1436527/1‑69 (MQ=255) atcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATc > 1:3371239/1‑69 (MQ=255) atcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATc > 1:2124240/1‑69 (MQ=255) atcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATc > 1:2060158/1‑69 (MQ=255) atcGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATc > 1:549280/1‑69 (MQ=255) atcGCGGCCCACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATc < 1:898334/69‑1 (MQ=255) atcGCGGCCCACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATc < 1:2878666/69‑1 (MQ=255) atcGCGGCCCACTTCAAAACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATc < 1:1885124/69‑1 (MQ=255) cgGCCCACTTCAATGCGGTAGAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGAct < 1:2152240/62‑2 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGt > 1:469296/1‑68 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:2723583/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:2229389/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:2195745/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:41779/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:583208/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:588052/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:775405/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:822780/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:85258/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:1793513/1‑69 (MQ=255) cACTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTc > 1:882772/1‑69 (MQ=255) ctacAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:2184122/4‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGCCGCGGACGATCtt > 1:1487737/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:2363160/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:308429/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1297345/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1197606/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:2447696/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:392703/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:434587/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:2256770/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1136503/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1981186/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:650234/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:677335/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1914801/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1811332/1‑60 (MQ=255) cTTCAATACGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1693338/1‑60 (MQ=255) cTTCAATACGGTAAAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCtt > 1:1011348/1‑60 (MQ=255) tCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGAcgcg < 1:597760/49‑1 (MQ=255) tCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGAcgcg < 1:2265665/49‑1 (MQ=255) tCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGAcgcg < 1:2790212/49‑1 (MQ=255) atacGGTACAACTGCATATCGCTAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:209339/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:1677541/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:2607180/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:2625988/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:2014992/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:2293029/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:3143286/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:1358239/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:2945428/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:1355634/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCAcgc > 1:2336374/4‑69 (MQ=255) atacGGTACAACTGCATATCGCCAACATCACGACGTTCGAGACGCGGACGATCTTCACGGTCGCCAcgc > 1:1885876/4‑69 (MQ=255) gTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCAcgcg > 1:2517703/1‑65 (MQ=255) gTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGcc > 1:2462906/1‑69 (MQ=255) gTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGcc > 1:3014823/1‑69 (MQ=255) gTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGcc > 1:156328/1‑69 (MQ=255) gTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGcc > 1:1843613/1‑69 (MQ=255) gTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGcc > 1:3219501/1‑69 (MQ=255) gTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGcc > 1:2689184/1‑69 (MQ=255) gTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGcc > 1:1532445/1‑69 (MQ=255) gTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGcc > 1:3409820/1‑69 (MQ=255) gTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGcc > 1:3402429/1‑69 (MQ=255) aCAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGCCAc > 1:2501890/1‑69 (MQ=255) gCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGCCACGGTCGt > 1:2531174/1‑69 (MQ=255) gCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGCCACGGTCGt > 1:1100185/1‑69 (MQ=255) cATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGCCACGGTCGtt > 1:2674873/1‑69 (MQ=255) cATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGCCACGGTCGtt > 1:1841395/1‑69 (MQ=255) cATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGCCACGGTCGtt > 1:1547035/1‑69 (MQ=255) cATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGCCACGGTCGtt > 1:1642680/1‑69 (MQ=255) tCGCCAACATCACGACGTTCACGACGCGGACGCTCTTCACGGTCACCACGCGGGCCACGGTCGTTACGa > 1:1126466/1‑69 (MQ=255) tCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGCCACGGTCGTTACGa > 1:789913/1‑69 (MQ=255) tCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGCCACGGTCGTTACGa > 1:460415/1‑69 (MQ=255) tCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGCCACGGTCGTTACGa > 1:2989062/1‑69 (MQ=255) aCATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCACGCGGGCCAc < 1:2820790/52‑1 (MQ=39) aCATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCACGCGGGCCAc < 1:159138/52‑1 (MQ=39) aCATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCACGCGGGCCAc < 1:1928788/52‑1 (MQ=39) aCATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCACGCGGGCCAc < 1:721049/52‑1 (MQ=39) aCATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCACGCGGGCCAc < 1:2381557/52‑1 (MQ=39) aCATCACGACGTTCGCGACGCGGACGATCTTCACGGTCGCCACGCGGGCCAc < 1:2327116/52‑1 (MQ=39) gTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGCCACGGTCGTTACGATCGCGCGGACCACGGt < 1:3261997/69‑1 (MQ=255) cACGACGCGGACGATCTTCACGGTCACCACGCGGGCCACGGTCGTTACGATCGCGCGGACCACGGtcat > 1:2177136/1‑69 (MQ=255) cACGACGCGGACGATCTTCACGGTCACCACGCGGGCCACGGTCGTTACGATCGCGCGGACCACGGtcat > 1:2215595/1‑69 (MQ=255) cACGACGCGGACGATCTTCACGGTCACCACGCGGGCCACGGTCGTTACGATCGCGCGGACCACGGtcat > 1:1293415/1‑69 (MQ=255) cACGACGCGGACGATCTTCACGGTCACCACGCGGGCCACGGTCGTTACGATCGCGCGGACCACGGtcat > 1:3201788/1‑69 (MQ=255) | TTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGACGTTCACGACGCGGACGATCTTCACGGTCACCACGCGGGCCACGGTCGTTACGATCGCGCGGACCACGGTCAT > minE/2131448‑2131581 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |