| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 182,379 | C→A | 44.4% | L69L (CTC→CTA) | lpxB → | tetraacyldisaccharide‑1‑P synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 182,379 | 0 | C | A | 44.4% | 30.4 / 69.8 | 72 | L69L (CTC→CTA) | lpxB | tetraacyldisaccharide‑1‑P synthase |
| Reads supporting (aligned to +/- strand): ref base C (32/8); new base A (10/22); total (42/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.91e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.63e-01 | |||||||||||
TGAAGGCTGCGAAGCCTGGTACGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAGCGTTTTGGCGAACTGAA > minE/182316‑182441 | tGAAGGCTGCGAAGCCTGGTACGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGgtcg > 1:1074496/1‑69 (MQ=255)tGAAGGCTGCGAAGCCTGGTACGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGgtcg > 1:2617070/1‑69 (MQ=255)tGAAGGCTGCGAAGCCTGGTACGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGgtcg > 1:2721435/1‑69 (MQ=255)tGAAGGCTGCGAAGCCTGGTACGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGgtcg > 1:1588546/1‑69 (MQ=255)tGAAGGCTGCGAAGCCTGGTACGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGgtcg > 1:1560465/1‑69 (MQ=255)tGAAGGCTGCGAAGCCTGGTACGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGgtc > 1:2928873/1‑68 (MQ=255)tGAAGGCTGCGAAGCCTGGTACGAAATGGAAGAACTGGCGGTAATGGGCATTGTTGAAGTGATCGgtcg > 1:1724390/1‑69 (MQ=255) cTGGTACGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTAc > 1:564371/1‑68 (MQ=255) cTGGTACGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTAc > 1:525736/1‑68 (MQ=255) cTGGTACGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTAc > 1:3072246/1‑68 (MQ=255) ggTACGAAATGTAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:2404857/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:1518206/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:2581327/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:2561865/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:1218694/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:275131/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:2264320/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:2755549/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:2943150/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:2974391/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:210536/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:1947772/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:1907880/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:1872042/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:350436/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:424668/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:1601130/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:967624/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:604493/69‑3 (MQ=255) ggTACGAAATGGAAGAACTGGAAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:1604869/69‑3 (MQ=255) ggTACGAAATGCAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:3077004/69‑3 (MQ=255) gTACGAAATGGAAGAACTGGCAGTGATGGGCATTGTTGAAGTGCTAGGTCGTCTGCGTCGCTTACtac < 1:1904029/68‑3 (MQ=255) aCGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCata > 1:354462/1‑69 (MQ=255) aCGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCat > 1:1074764/1‑68 (MQ=255) aCGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCat > 1:2332994/1‑68 (MQ=255) aCGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCa > 1:2641301/1‑67 (MQ=255) aCGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCa < 1:2650018/67‑1 (MQ=255) aCGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCa < 1:2654106/67‑1 (MQ=255) aCGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCa < 1:1726067/67‑1 (MQ=255) aTGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCg > 1:384752/1‑68 (MQ=255) tGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTg > 1:2154268/1‑69 (MQ=255) gagATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAg > 1:762415/3‑69 (MQ=255) gTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAg > 1:2600311/1‑69 (MQ=255) gTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAg > 1:2184584/1‑69 (MQ=255) gTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAg > 1:2830260/1‑69 (MQ=255) gTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAg > 1:3080751/1‑69 (MQ=255) gTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAg > 1:270177/1‑69 (MQ=255) gATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAGCg > 1:1500394/1‑69 (MQ=255) gATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAGCg > 1:2644657/1‑69 (MQ=255) gATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAGCg > 1:2955589/1‑69 (MQ=255) gATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAGCg > 1:1938556/1‑69 (MQ=255) gATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGACGATCTGACAAAGCg > 1:2639700/1‑69 (MQ=255) ttgttgAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAGCGTTTTGGCg < 1:2640451/69‑1 (MQ=255) ttgttgAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAGCGTTTTGGCg < 1:1458332/69‑1 (MQ=255) ttgtggAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAGCGTTTTGGCg < 1:1705895/69‑1 (MQ=255) gttgAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAGCGTTTTGGCGaa > 1:734644/1‑69 (MQ=255) gAAGTGCTAGGTCGTCTGCGTCGCTTACTACATATTCGTGCCGATCTGACAAAGCGTTTTGGCGaat > 1:447495/1‑66 (MQ=255) gAAGTGCTAGGTCGTCTGCGTCGCTTACTACATATTCGTGCCGATCTGACAAAGCGTTTTGGCGaat > 1:640680/1‑66 (MQ=255) gAAGTGCTAGGTCGTCTGCGTCGCTTACTACATATTCGTGCCGATCTGACAAAGCGTTTTGGCGaat > 1:581589/1‑66 (MQ=255) gAAGTGCTAGGTCGTCTGCGTCGCTTACTACATATTCGTGCCGATCTGACAAAGCGTTTTGGCGaat > 1:565873/1‑66 (MQ=255) gAAGTGCTAGGTCGTCTGCGTCGCTTACTACATATTCGTGCCGATCTGACAAAGCGTTTTGGCGaat > 1:2471613/1‑66 (MQ=255) gAAGTGCTAGGTCGTCTGCGTCGCTTACTACATATTCGTGCCGATCTGACAAAGCGTTTTGGCGaat > 1:1641054/1‑66 (MQ=255) gAAGTGCTAGGTCGTCTGCGTCGCTTACTACATATTCGTGCCGATCTGACAAAGCGTTTTGGCGaat > 1:2224512/1‑66 (MQ=255) gAAGTGCTAGGTCGTCTGCGTCGCTTACTACATATTCGTGCCGATCTGACAAAGCGTTTTGGCGaat > 1:2803553/1‑66 (MQ=255) gAAGTGCTAGGTCGTCTGCGTCGCTTACTACATATTCGTGCCGATCTGACAAAGCGTTTTGGCGaat > 1:3359305/1‑66 (MQ=255) gAAGTGCTAGGTCGTCTGCGTCGCTTACTACATATTCGTGCCGATCTGACAAAGCGTTTTGGCGaat > 1:1309881/1‑66 (MQ=255) tGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAGCGTTTTGGCGAACTGaa > 1:2984999/1‑67 (MQ=255) tGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAGCGTTTTGGCGAACTGaa > 1:2813428/1‑67 (MQ=255) tGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAGCGTTTTGGCGAACTGaa > 1:650294/1‑67 (MQ=255) tGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAGCGTTTTGGCGAACTGaa > 1:1745613/1‑67 (MQ=255) cTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAGCGTTTTGGCGAACTGa < 1:1589145/64‑1 (MQ=255) cTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAGCGTTTTGGCGAACTGa < 1:212292/64‑1 (MQ=255) | TGAAGGCTGCGAAGCCTGGTACGAAATGGAAGAACTGGCGGTGATGGGCATTGTTGAAGTGCTCGGTCGTCTGCGTCGCTTACTGCATATTCGTGCCGATCTGACAAAGCGTTTTGGCGAACTGAA > minE/182316‑182441 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |