| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,131,628 | T→A | 37.8% | V440V (GTA→GTT) | deaD ← | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,131,628 | 0 | T | A | 37.8% | 60.0 / 77.9 | 91 | V440V (GTA→GTT) | deaD | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base T (7/49); new base A (16/18); total (23/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01 | |||||||||||
CGCGCGGACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTC > minE/2131564‑2131696 | cgcgcgGACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACg > 1:233083/1‑68 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACTTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:681444/69‑1 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:985030/69‑1 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:1003696/69‑1 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:945528/69‑1 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:936805/69‑1 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:810480/69‑1 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:3370109/69‑1 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:308984/69‑1 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:2698451/69‑1 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:2577112/69‑1 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:2562535/69‑1 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:2282725/69‑1 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:2278245/69‑1 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:1949270/69‑1 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:1650883/69‑1 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:135234/69‑1 (MQ=255) ggACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:1008025/69‑1 (MQ=255) gACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCagag < 1:2266189/69‑1 (MQ=255) gACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCagag < 1:1262940/69‑1 (MQ=255) gACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCagag < 1:1202477/69‑1 (MQ=255) gACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCagag < 1:2673086/69‑1 (MQ=255) gACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCagag < 1:1964443/69‑1 (MQ=255) gACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCagag < 1:1410176/69‑1 (MQ=255) gACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCagag < 1:2885480/69‑1 (MQ=255) gACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCagag < 1:49667/69‑1 (MQ=255) gACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCagag < 1:2917837/69‑1 (MQ=255) gACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCAGCGGCGCATCTGGCGGTACGATCagag < 1:2382241/69‑1 (MQ=255) aCCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCagag < 1:1560800/68‑1 (MQ=255) ccACGGTCCTCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTa < 1:1590888/69‑1 (MQ=255) ccACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTa < 1:372090/69‑1 (MQ=255) ccACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTa < 1:674513/69‑1 (MQ=255) ccACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTa < 1:1699899/69‑1 (MQ=255) ccACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTa < 1:762488/69‑1 (MQ=255) ccACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTa < 1:785268/69‑1 (MQ=255) ccACGGTCATCACGGGCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTa < 1:1069250/69‑1 (MQ=255) ggAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGAACAATCaga < 1:333731/47‑1 (MQ=38) gAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCAtt > 1:55222/1‑67 (MQ=255) gAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCAtt > 1:743631/1‑67 (MQ=255) gAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCAtt > 1:1007971/1‑67 (MQ=255) gAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCAtt > 1:1914788/1‑67 (MQ=255) tCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTcagc < 1:2495039/69‑1 (MQ=255) tCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTcagc < 1:1780333/69‑1 (MQ=255) tCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTcagc < 1:1143271/69‑1 (MQ=255) tCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTcagc < 1:1777987/69‑1 (MQ=255) tCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTcagc < 1:1787026/69‑1 (MQ=255) tCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTcagc < 1:872856/69‑1 (MQ=255) tCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTcagc < 1:549695/69‑1 (MQ=255) tCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTcagc < 1:2178042/69‑1 (MQ=255) tCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTcagc < 1:938233/69‑1 (MQ=255) tCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTcagc < 1:1360185/69‑1 (MQ=255) tCACGTTTCGGACGCATCGGCGCATCTGGCGGCACGATCAGAGTACGTTCACCCTGTGCCATTTTcagc < 1:3113697/69‑1 (MQ=255) cACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCag < 1:2557811/40‑1 (MQ=255) cACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCag < 1:1066656/40‑1 (MQ=255) cACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCag < 1:2661269/40‑1 (MQ=255) cACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCag < 1:1671903/40‑1 (MQ=255) cACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCag < 1:654655/40‑1 (MQ=255) cACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCag < 1:2830355/40‑1 (MQ=255) cACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCag < 1:2209977/40‑1 (MQ=255) cACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCag < 1:321292/40‑1 (MQ=255) cACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTcagc < 1:921142/68‑1 (MQ=255) cGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCag < 1:29367/38‑1 (MQ=255) cGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTcagcag < 1:1520946/68‑1 (MQ=255) cGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTcagcag < 1:1433731/68‑1 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGAACAATCAGAGTACGTTCACCCTGTGCCATTTTCTGTAGTg > 1:2693012/1‑69 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGAACAATCAGAGTACGTTCACCCTGTGCCATTTTCAGTAGTg > 1:852048/1‑69 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGAACAATCAGAGTACGTTCACCCTGTGCCATTTTCAGTAGTg > 1:8987/1‑69 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGAACAATCAGAGTACGTTCACCCTGTGCCATTTTCAGTAGTg > 1:1091566/1‑69 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGAACAATCAGAGTACGTTCACCCTGTGCCATTTTCAGTAGTg > 1:101896/1‑69 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGAACAATCAGAGTACGTTCACCCTGTGCCATTTTCAGTAGTg > 1:1104855/1‑69 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGAACAATCAGAGTACGTTCACCCTGTGCCATTTTCAGTAGTg > 1:3339190/1‑69 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGAACAATCAGAGTACGTTCACCCTGTGCCATTTTCAGTAGTg > 1:2257388/1‑69 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGAACAATCAGAGTACGTTCACCCTGTGCCATTTTCAGTAGTg > 1:2304490/1‑69 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGAACAATCAGAGTACGTTCACCCTGTGCCATTTTCAGTAGTg > 1:2322896/1‑69 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGAACAATCAGAGTACGTTCACCCTGTGCCATTTTCAGTAGTg > 1:304912/1‑69 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGAACAATCAGAGTACGTTCACCCTGTGCCATTTTCAGTAGTg > 1:3003031/1‑69 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGAACAATCAGAGTACGTTCACCCTGTGCCATTTTCAGTAGTg > 1:2347318/1‑69 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGAACAATCAGAGTACGTTCACCCTGTGCCATTTTCAGTAGTg > 1:2009051/1‑69 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGAACAATCAGAGTACGTTCACCCTGTGCCATTTTCAGTAGTg > 1:2449025/1‑69 (MQ=255) gTTTCGGACGCATCGGCGCATCTGGCGGAACAATCAGAGTACGTTCACCCTGTGCCATTTTCAGTAGTg > 1:2087393/1‑69 (MQ=255) ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCa < 1:1650478/64‑1 (MQ=255) ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCa < 1:2096351/64‑1 (MQ=255) ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCa < 1:779512/64‑1 (MQ=255) ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCa < 1:1867179/64‑1 (MQ=255) ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCa < 1:751095/64‑1 (MQ=255) ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCa < 1:2663161/64‑1 (MQ=255) ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCa < 1:2835371/64‑1 (MQ=255) ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCa < 1:1797287/64‑1 (MQ=255) ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCa < 1:1808993/64‑1 (MQ=255) ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGtt > 1:1178464/1‑69 (MQ=255) ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGtt > 1:188815/1‑69 (MQ=255) tACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGctc < 1:1651324/69‑1 (MQ=255) tACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGctc < 1:2187570/69‑1 (MQ=255) tACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGctc < 1:2257221/69‑1 (MQ=255) | CGCGCGGACCACGGTCATCACGGTCACGGAATTCACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTC > minE/2131564‑2131696 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |