| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,135,734 | A→G | 28.7% | D199D (GAT→GAC) | pnp ← | polynucleotide phosphorylase/polyadenylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,135,734 | 0 | A | G | 28.7% | 55.9 / 24.3 | 59 | D199D (GAT→GAC) | pnp | polynucleotide phosphorylase/polyadenylase |
| Reads supporting (aligned to +/- strand): ref base A (24/18); new base G (14/3); total (38/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.00e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGCTTCAGATTCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTA > minE/2135682‑2135796 | gAGCTTCAGATTCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGctctct > 1:1549850/1‑68 (MQ=255) cAGATTCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCt > 1:1056027/1‑69 (MQ=255) cAGATTCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCt > 1:3323665/1‑69 (MQ=255) cAGATTCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCt > 1:3149275/1‑69 (MQ=255) cAGATTCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCt > 1:2439493/1‑69 (MQ=255) cAGATTCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCt > 1:606833/1‑69 (MQ=255) cAGATTCAACCATCAGCGCGGCAGCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAgt > 1:3412704/1‑67 (MQ=255) cAGATTCAACCATCAGCACGGCAGCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAgt > 1:386978/1‑67 (MQ=255) cAGATTCAACCATCAGCACGGCAGCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAgt > 1:1480017/1‑67 (MQ=255) cAGATTCAACCATCAGCACGGCAGCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAgt > 1:1533707/1‑67 (MQ=255) cAGATTCAACCATCAGCACGGCAGCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAgt > 1:733553/1‑67 (MQ=255) cAGATTCAACCATCAGCACGGCAGCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAgt > 1:3053334/1‑67 (MQ=255) cAGATTCAACCATCAGCACGGCAGCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAgt > 1:959368/1‑67 (MQ=255) cAGATTCAACCATCAGCACGGCAGCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAgt > 1:1221387/1‑67 (MQ=255) cAGATTCAACCATCAGCACGGCAGCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAgt > 1:1869535/1‑67 (MQ=255) cAGATTCAACCATCAGCACGGCAGCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAgt > 1:2049678/1‑67 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:2723163/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:1025391/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:584514/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:1200139/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:3085583/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:2089714/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:1927721/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:1683121/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:3280371/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:1601358/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:156297/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:1461641/1‑68 (MQ=255) tACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCggg > 1:1402851/1‑67 (MQ=255) tACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCggg > 1:2489553/1‑67 (MQ=255) tACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGATTGCTCTCTTTCAGCTCGTCCTGAGTCgg > 1:2985779/1‑66 (MQ=255) aCAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTc < 1:353479/69‑1 (MQ=255) aCAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTc < 1:509243/69‑1 (MQ=255) aCAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTc < 1:1380444/69‑1 (MQ=255) aCAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTc < 1:2602134/69‑1 (MQ=255) aCAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTc < 1:2510639/69‑1 (MQ=255) aCAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTc < 1:2133808/69‑1 (MQ=255) cAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGtt > 1:609463/1‑67 (MQ=255) cAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTc > 1:2996831/1‑68 (MQ=255) cAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTc > 1:2253520/1‑68 (MQ=255) gcagCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAGTTCGTCCTGAGTCGGGTTCAg < 1:2853806/65‑1 (MQ=255) gcagCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAGTTCGTCCTGAGTCGGGTTCAg < 1:1610732/65‑1 (MQ=255) cagCTTCTGTACCCGCAACAACCAGGTCCAGTTTGCTCTCTTTCAGTTCGTCCTGAGTCGGGTTCAg < 1:1189672/65‑1 (MQ=255) agCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAGTTCGTCCTGAGTCg > 1:247428/2‑59 (MQ=255) agCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAGTTCGTCCTGAGTCGGGTTCAgta > 1:639637/2‑68 (MQ=255) agCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAGTTCGTCCTGAGTCGGGTTCAgta > 1:2486931/2‑68 (MQ=255) agCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAGTTCGTCCTGAGTCGGGTTCAgta > 1:2452657/2‑68 (MQ=255) gCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAgtacg < 1:1070261/69‑1 (MQ=255) gCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAgtacg < 1:998097/69‑1 (MQ=255) cTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAgtacgt < 1:1347906/69‑1 (MQ=255) cTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAgtacgt < 1:978676/69‑1 (MQ=255) caacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAgtacgt < 1:535915/54‑1 (MQ=255) aacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTa < 1:3381566/69‑1 (MQ=255) aacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTa < 1:3001081/69‑1 (MQ=255) aacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTa < 1:2905116/69‑1 (MQ=255) aacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTa < 1:1655022/69‑1 (MQ=255) aacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTa < 1:1625837/69‑1 (MQ=255) aacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTa < 1:680677/69‑1 (MQ=255) aacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTa < 1:1284892/69‑1 (MQ=255) | GAGCTTCAGATTCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTA > minE/2135682‑2135796 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |