| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,135,755 | C→T | 14.3% | E192E (GAG→GAA) | pnp ← | polynucleotide phosphorylase/polyadenylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,135,755 | 0 | C | T | 14.3% | 96.4 / 4.9 | 55 | E192E (GAG→GAA) | pnp | polynucleotide phosphorylase/polyadenylase |
| Reads supporting (aligned to +/- strand): ref base C (24/23); new base T (4/4); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
CAGATTCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCA > minE/2135688‑2135815 | cAGATTCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCt > 1:1056027/1‑69 (MQ=255)cAGATTCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCt > 1:606833/1‑69 (MQ=255)cAGATTCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCt > 1:2439493/1‑69 (MQ=255)cAGATTCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCt > 1:3149275/1‑69 (MQ=255)cAGATTCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCt > 1:3323665/1‑69 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:3280371/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:2723163/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:1025391/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:2089714/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:3085583/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:1927721/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:1683121/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:1601358/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:1461641/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:156297/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:1200139/1‑68 (MQ=255) tCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTc > 1:584514/1‑68 (MQ=255) tACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCggg > 1:1402851/1‑67 (MQ=255) tACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCggg > 1:2489553/1‑67 (MQ=255) tACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGATTGCTCTCTTTCAGCTCGTCCTGAGTCgg > 1:2985779/1‑66 (MQ=255) aCAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTc < 1:2602134/69‑1 (MQ=255) aCAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTc < 1:2510639/69‑1 (MQ=255) aCAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTc < 1:2133808/69‑1 (MQ=255) aCAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTc < 1:509243/69‑1 (MQ=255) aCAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTc < 1:353479/69‑1 (MQ=255) aCAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTc < 1:1380444/69‑1 (MQ=255) cAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGtt > 1:609463/1‑67 (MQ=255) cAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTc > 1:2253520/1‑68 (MQ=255) cAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTc > 1:2996831/1‑68 (MQ=255) gcagCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAGTTCGTCCTGAGTCGGGTTCAg < 1:2853806/65‑1 (MQ=255) gcagCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAGTTCGTCCTGAGTCGGGTTCAg < 1:1610732/65‑1 (MQ=255) cagCTTCTGTACCCGCAACAACCAGGTCCAGTTTGCTCTCTTTCAGTTCGTCCTGAGTCGGGTTCAg < 1:1189672/65‑1 (MQ=255) agCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAGTTCGTCCTGAGTCg > 1:247428/2‑59 (MQ=255) agCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAGTTCGTCCTGAGTCGGGTTCAgta > 1:2486931/2‑68 (MQ=255) agCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAGTTCGTCCTGAGTCGGGTTCAgta > 1:639637/2‑68 (MQ=255) agCTTCTGTACCGGCAACAACCAGGTCCAGTTTGCTCTCTTTCAGTTCGTCCTGAGTCGGGTTCAgta > 1:2452657/2‑68 (MQ=255) gCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAgtacg < 1:998097/69‑1 (MQ=255) gCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAgtacg < 1:1070261/69‑1 (MQ=255) cTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAgtacgt < 1:1347906/69‑1 (MQ=255) cTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAgtacgt < 1:978676/69‑1 (MQ=255) caacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAgtacgt < 1:535915/54‑1 (MQ=255) aacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTa < 1:3001081/69‑1 (MQ=255) aacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTa < 1:1655022/69‑1 (MQ=255) aacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTa < 1:1625837/69‑1 (MQ=255) aacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTa < 1:3381566/69‑1 (MQ=255) aacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTa < 1:1284892/69‑1 (MQ=255) aacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTa < 1:680677/69‑1 (MQ=255) aacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTa < 1:2905116/69‑1 (MQ=255) ggtCCAGTTTGCTCTCTTTCAGTTCGTCCTGAGTCGGGTTCAg < 1:1483105/41‑1 (MQ=39) gTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTAcgcg > 1:3256661/1‑67 (MQ=255) ctctTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCAcc < 1:1970165/69‑1 (MQ=255) tttCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCa < 1:2370092/67‑1 (MQ=255) tttCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCa < 1:1090434/67‑1 (MQ=255) tttCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCa < 1:124337/67‑1 (MQ=255) tttCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCa < 1:2533018/67‑1 (MQ=255) | CAGATTCAACCATCAGTACAGCGGCTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCA > minE/2135688‑2135815 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |