| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,137,295 | G→A | 47.2% | G192G (GGC→GGT) | truB ← | tRNA pseudouridine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,137,295 | 0 | G | A | 47.2% | 6.1 / 60.9 | 72 | G192G (GGC→GGT) | truB | tRNA pseudouridine synthase |
| Reads supporting (aligned to +/- strand): ref base G (15/23); new base A (18/16); total (33/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCC > minE/2137228‑2137361 | tCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAg > 1:786468/1‑68 (MQ=255)tCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAg > 1:1805295/1‑68 (MQ=255)tCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAg > 1:1575008/1‑68 (MQ=255) tCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGtt > 1:2445072/1‑69 (MQ=255) tCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGtt > 1:256026/1‑69 (MQ=255) tCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGtt > 1:3070135/1‑69 (MQ=255) tCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGtt > 1:436142/1‑69 (MQ=255) tCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGtt > 1:836113/1‑69 (MQ=255) tCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCAAAGCCGAGtt > 1:1754110/1‑69 (MQ=255) tCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGAAGGTAAATAACATGCGCACCACAGCCGAGtt > 1:1984346/1‑69 (MQ=255) cGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGttt > 1:1304741/1‑68 (MQ=255) cGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGttt > 1:1794710/1‑68 (MQ=255) cGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGttt > 1:1690766/1‑68 (MQ=255) ttCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGttttt < 1:962993/68‑1 (MQ=255) ttCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGttttt < 1:1597108/68‑1 (MQ=255) ttCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGctttt < 1:276418/68‑1 (MQ=255) ccGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCAccc < 1:2800029/68‑1 (MQ=255) ccGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCAccc < 1:1918714/68‑1 (MQ=255) ccGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCAccc < 1:2911770/68‑1 (MQ=255) tttACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGtcatc > 1:1122222/1‑69 (MQ=255) tttACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGtcat > 1:727160/1‑68 (MQ=255) acttacCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGtcatca < 1:1596268/67‑1 (MQ=255) ccAGACGACGCAAGTAAATAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAatga < 1:650198/63‑1 (MQ=255) ccAGACGACGCAAGTAAATAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAatga < 1:3233151/63‑1 (MQ=255) ccAGACGACGCAAGTAAATAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAatga < 1:3381719/63‑1 (MQ=255) ccAGACGACGCAAGTAAATAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAatga < 1:709390/63‑1 (MQ=255) ccAGACGACGCAAGTAAATAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAatga < 1:2561199/63‑1 (MQ=255) ccAGACGACGCAAGTAAATAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAatga < 1:746069/63‑1 (MQ=255) ccAGACGACGCAAGTAAATAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAatga < 1:839282/63‑1 (MQ=255) ccAGACGACGCAAGTAAATAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAatga < 1:3135169/63‑1 (MQ=255) ccAGACGACGCAAGTAAATAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAatga < 1:940054/63‑1 (MQ=255) ccAGACGACGCAAGTAAATAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAatga < 1:1031119/63‑1 (MQ=255) ccAGACGACGCAAGTAAATAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAatga < 1:1195247/63‑1 (MQ=255) ccAGACGACGCAAGTAAATAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAatga < 1:1215347/63‑1 (MQ=255) ccAGACGACGCAAGTAAATAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAatga < 1:1852467/63‑1 (MQ=255) ccAGACGACGCAAGTAAATAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAatga < 1:1573370/63‑1 (MQ=255) ccAGACGACGCAAGTAAATAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAatga < 1:1660680/63‑1 (MQ=255) gACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGa < 1:968483/69‑1 (MQ=255) gACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGa < 1:2423797/69‑1 (MQ=255) ggCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGatat < 1:1082734/69‑1 (MQ=255) gcgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGatat < 1:2261311/68‑1 (MQ=255) gcgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGatat < 1:1746818/68‑1 (MQ=255) gcgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGAtata < 1:2173170/69‑1 (MQ=255) gcgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGAtata < 1:166751/69‑1 (MQ=255) gcgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGAtata < 1:2297205/69‑1 (MQ=255) cgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGAtata < 1:1760907/68‑1 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:1292105/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:749617/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:1099491/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:658112/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:1886243/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:582229/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:1876516/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:355264/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:2788442/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:3308389/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:1338693/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:3160136/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:1363783/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:1453502/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:3134899/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:194312/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTCCGGATATAAGTCCCtttt > 1:611783/1‑68 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCCTCAATGATAGTGCGGATAAAAGTGCCTTTTg > 1:3351491/1‑69 (MQ=255) aTGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAatga < 1:3383302/41‑1 (MQ=19) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg > 1:991624/1‑69 (MQ=255) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg < 1:882267/69‑1 (MQ=255) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg < 1:793578/69‑1 (MQ=255) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg < 1:315293/69‑1 (MQ=255) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg > 1:3088524/1‑69 (MQ=255) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg < 1:2206041/69‑1 (MQ=255) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg < 1:1095463/69‑1 (MQ=255) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg > 1:1065793/1‑69 (MQ=255) caGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTtcc < 1:2040328/69‑1 (MQ=255) caGCCGAGTTTTTCACCCAGGGCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTtcc < 1:2767693/69‑1 (MQ=255) | TCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCC > minE/2137228‑2137361 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |