| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,137,325 | G→A | 49.2% | T182T (ACC→ACT) | truB ← | tRNA pseudouridine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,137,325 | 0 | G | A | 49.2% | ‑2.0 / 59.0 | 67 | T182T (ACC→ACT) | truB | tRNA pseudouridine synthase |
| Reads supporting (aligned to +/- strand): ref base G (3/31); new base A (18/15); total (21/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.95e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01 | |||||||||||
GACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCG > minE/2137264‑2137387 | gACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGa < 1:2423797/69‑1 (MQ=255)gACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGa < 1:968483/69‑1 (MQ=255) ggCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGatat < 1:1082734/69‑1 (MQ=255) gcgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGatat < 1:2261311/68‑1 (MQ=255) gcgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGatat < 1:1746818/68‑1 (MQ=255) gcgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGAtata < 1:2297205/69‑1 (MQ=255) gcgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGAtata < 1:2173170/69‑1 (MQ=255) gcgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGAtata < 1:166751/69‑1 (MQ=255) cgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGAtata < 1:1760907/68‑1 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:355264/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:2788442/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:3134899/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:1099491/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:3160136/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:1292105/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:3308389/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:1338693/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:194312/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:582229/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:1886243/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:1876516/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:1363783/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:1453502/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:658112/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTGCGGATATAAGTGCCTTTTg > 1:749617/1‑69 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCGTCAATGATAGTCCGGATATAAGTCCCtttt > 1:611783/1‑68 (MQ=255) tAACATGCGCGCCACAACCGAGTTTTTCGCCGAGATCCTCAATGATAGTGCGGATAAAAGTGCCTTTTg > 1:3351491/1‑69 (MQ=255) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg < 1:882267/69‑1 (MQ=255) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg > 1:991624/1‑69 (MQ=255) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg < 1:315293/69‑1 (MQ=255) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg > 1:1065793/1‑69 (MQ=255) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg > 1:3088524/1‑69 (MQ=255) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg < 1:793578/69‑1 (MQ=255) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg < 1:1095463/69‑1 (MQ=255) cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTg < 1:2206041/69‑1 (MQ=255) caGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTtcc < 1:2040328/69‑1 (MQ=255) caGCCGAGTTTTTCACCCAGGGCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTtcc < 1:2767693/69‑1 (MQ=255) ccGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTtccagc < 1:890786/69‑1 (MQ=255) ccGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTtccagc < 1:3264520/69‑1 (MQ=255) aCCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCAt < 1:188888/68‑1 (MQ=255) aGGTCATCAATGATGGTGCGGTTATAAGTGCCTTTTGAGCAGTGTTTTtcc < 1:437214/51‑1 (MQ=255) ggTCATCAATGATGGTGCTGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCtt < 1:2847361/69‑1 (MQ=255) ggTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCtt < 1:1700469/69‑1 (MQ=255) ggTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCtt < 1:2747634/69‑1 (MQ=255) ggTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCtt < 1:954193/69‑1 (MQ=255) ggTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCtt < 1:860468/69‑1 (MQ=255) ggTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCtt < 1:1597362/69‑1 (MQ=255) ggTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCtt < 1:3075196/69‑1 (MQ=255) ggTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCtt < 1:2666655/69‑1 (MQ=255) ggTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCtt < 1:2704888/69‑1 (MQ=255) ggTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCtt < 1:2294807/69‑1 (MQ=255) gTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTtcc < 1:2369379/49‑1 (MQ=255) tcgtcaATGATAGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCa < 1:855015/66‑1 (MQ=255) tcgtcaATGATAGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCa < 1:1374887/66‑1 (MQ=255) tcgtcaATGATAGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCa < 1:237570/66‑1 (MQ=255) tcgtcaATGATAGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCa < 1:1551478/66‑1 (MQ=255) tcgtcaATGATAGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCa < 1:755335/66‑1 (MQ=255) tcgtcaATGATAGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCa < 1:1636474/66‑1 (MQ=255) tcgtcaATGATAGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCa < 1:652050/66‑1 (MQ=255) aaTGATAGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCg < 1:3187561/69‑1 (MQ=255) aaTGATAGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCg < 1:444869/69‑1 (MQ=255) aaTGATAGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCg < 1:861462/69‑1 (MQ=255) aaTGATAGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCg < 1:1525028/69‑1 (MQ=255) aaTGATAGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCg < 1:884066/69‑1 (MQ=255) aaTGATAGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCg < 1:611364/69‑1 (MQ=255) aaTGATAGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCg < 1:989011/69‑1 (MQ=255) aaTGATAGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCg < 1:540902/69‑1 (MQ=255) gataGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCg < 1:2005173/66‑1 (MQ=255) | GACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCG > minE/2137264‑2137387 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |