| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,137,703 | C→T | 44.7% | P56P (CCG→CCA) | truB ← | tRNA pseudouridine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,137,703 | 0 | C | T | 44.7% | 6.8 / 35.8 | 47 | P56P (CCG→CCA) | truB | tRNA pseudouridine synthase |
| Reads supporting (aligned to +/- strand): ref base C (9/17); new base T (10/11); total (19/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
GACCCGATAGCGTTTGTCGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACGGTTGGCGTTATATATAC > minE/2137637‑2137770 | gACCCGATAGCGTTTGTCGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCg > 1:723550/1‑68 (MQ=255) cGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACa > 1:2933594/1‑57 (MQ=255) cGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGcc > 1:1202689/1‑68 (MQ=255) cGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGcc > 1:2427154/1‑68 (MQ=255) cGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCa > 1:248700/1‑69 (MQ=255) cGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCa > 1:2774644/1‑69 (MQ=255) cGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCa > 1:2241759/1‑69 (MQ=255) cGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCa > 1:3076827/1‑69 (MQ=255) cGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCa > 1:3103088/1‑69 (MQ=255) cGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCa > 1:1631699/1‑69 (MQ=255) cGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCa > 1:1437143/1‑69 (MQ=255) ggAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAg > 1:3242077/1‑69 (MQ=255) ggAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAg > 1:1532233/1‑69 (MQ=255) aGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGc < 1:1892235/68‑1 (MQ=255) aGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGc < 1:2987336/68‑1 (MQ=255) aGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGc < 1:2213530/68‑1 (MQ=255) aGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGc < 1:2263035/68‑1 (MQ=255) tCCAGCAGATACTTGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCAGCggg < 1:403372/69‑1 (MQ=255) tCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCAGCggg < 1:2934147/69‑1 (MQ=255) tCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCAGCggg < 1:2904399/69‑1 (MQ=255) tCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCAGCggg < 1:2651428/69‑1 (MQ=255) tCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCAGCggg < 1:811803/69‑1 (MQ=255) tCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCAGCggg < 1:2457239/69‑1 (MQ=255) tCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCAGCggg < 1:2406011/69‑1 (MQ=255) tCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCAGCggg < 1:2385312/69‑1 (MQ=255) tCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCAGCggg < 1:2070335/69‑1 (MQ=255) tCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCAGCggg < 1:2038172/69‑1 (MQ=255) tCCAGCAGATACTGGGAAAACTTCGTCCCTTCCCCGAGGCAAATTGGCAACATGCCGGTCGCCAGCggg < 1:1261053/69‑1 (MQ=255) cagcagATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCggg > 1:2630768/1‑67 (MQ=255) cagcagATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCggg > 1:1582432/1‑67 (MQ=255) cagcagATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCggg > 1:1647377/1‑67 (MQ=255) cgCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCAc < 1:2378498/68‑1 (MQ=255) cgCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCAc < 1:3250483/68‑1 (MQ=255) cgCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCAc < 1:339455/68‑1 (MQ=255) cgCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCAc < 1:1453531/68‑1 (MQ=255) gCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACgg < 1:3059640/69‑1 (MQ=255) gCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACgg < 1:1713437/69‑1 (MQ=255) gCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACgg < 1:3427330/69‑1 (MQ=255) gCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACgg < 1:3430996/69‑1 (MQ=255) gCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACgg < 1:453644/69‑1 (MQ=255) gCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACgg < 1:564121/69‑1 (MQ=255) gCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACgg < 1:1022951/69‑1 (MQ=255) gCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACGGTTGGCGTtata < 1:3154104/69‑1 (MQ=255) gCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCAGGCACGGTTGGCGTtata < 1:348860/69‑1 (MQ=255) tCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACg > 1:1969315/1‑52 (MQ=255) tCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACGGTTGGCGTTATATATAc > 1:3027746/1‑69 (MQ=255) tCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACGGTTGGCGTTATATATAc > 1:2816559/1‑69 (MQ=255) | GACCCGATAGCGTTTGTCGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACGGTTGGCGTTATATATAC > minE/2137637‑2137770 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |