| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,138,498 | G→A | 59.7% | D870D (GAC→GAT) | infB ← | fused protein chain initiation factor 2, IF2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,138,498 | 0 | G | A | 59.7% | 47.1 / 82.1 | 114 | D870D (GAC→GAT) | infB | fused protein chain initiation factor 2, IF2 |
| Reads supporting (aligned to +/- strand): ref base G (17/29); new base A (13/55); total (30/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.02e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 126 total aligned reads displayed.
ATGGTACGTTGGATCTCGATGATTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAG > minE/2138442‑2138567 | aTGGTACGTTGGATCTCGATGATTTCGAATACTTCGATCACATCGCCAGTGCGGACGGCGTTGt < 1:2981636/64‑1 (MQ=255) ggTACGTTGGATCTCGATGATTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCtt < 1:1913671/69‑1 (MQ=255) ggTACGTTGGATCTCGATGATTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCtt < 1:2602775/69‑1 (MQ=255) ggTACGTTGGATCTCGATGATTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCtt < 1:1125848/69‑1 (MQ=255) ggTACGTTGGATCTCGATGATTTCGAATACTACGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCtt < 1:109417/69‑1 (MQ=255) ttGGATCTCGATGATTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCTTAACGcc < 1:2348349/69‑1 (MQ=255) ttGGATCTCGATGATTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCTTAACGcc < 1:270334/69‑1 (MQ=255) ttGGATCTCGATGATTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCTTAACGcc < 1:2350449/69‑1 (MQ=255) ggATCTCGATGATTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCtt < 1:200267/61‑1 (MQ=255) ggATCTCGATGATTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCtt < 1:1891262/61‑1 (MQ=255) ggATCTCGATGATTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCtt < 1:2890634/61‑1 (MQ=255) ggATCTCGATGATTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCtt < 1:1765429/61‑1 (MQ=255) ggATCTCGATGATTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCtt < 1:2244357/61‑1 (MQ=255) ggATCTCGATGATTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCtt < 1:2153820/61‑1 (MQ=255) ggATCTCGATGATTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCtt < 1:2442397/61‑1 (MQ=255) gatTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGt > 1:2235681/1‑44 (MQ=255) gatTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGt > 1:1087208/1‑44 (MQ=255) gatTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGt > 1:168939/1‑44 (MQ=255) gatTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGt > 1:160082/1‑44 (MQ=255) gatTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGt > 1:1704315/1‑44 (MQ=255) gatTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGt > 1:3205790/1‑44 (MQ=255) gatTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGt > 1:1710220/1‑44 (MQ=255) gatTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGt > 1:610767/1‑44 (MQ=255) gatTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGt > 1:1747710/1‑44 (MQ=255) atTTCGAATACTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTc > 1:113140/1‑68 (MQ=255) atTTCGAATACTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTc > 1:1671204/1‑68 (MQ=255) atTTCGAATACTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTc > 1:2343212/1‑68 (MQ=255) atTTCGAATACTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTc > 1:3075399/1‑68 (MQ=255) atTTCGAATACTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTc > 1:3083824/1‑68 (MQ=255) atTTCGAATACTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTc > 1:337582/1‑68 (MQ=255) atTTCGAATACTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTc > 1:13597/1‑68 (MQ=255) atTTCGAATACTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTc > 1:1319619/1‑68 (MQ=255) atTTCGAATACTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTc > 1:1251130/1‑68 (MQ=255) atTTCGAATACTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTc > 1:1186221/1‑68 (MQ=255) gAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCTTAACGCCGATACCACATTCCATAc < 1:3200202/68‑1 (MQ=255) gAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCTTAACGCCGATACCACATTCCATAc < 1:3290823/68‑1 (MQ=255) gAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCTTAACGCCGATACCACATTCCATAc < 1:1438723/68‑1 (MQ=255) gAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCTTAACGCCGATACCACATTCCATAc < 1:2944544/68‑1 (MQ=255) gAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCTTAACGCCGATACCACATTCCATAc < 1:500818/68‑1 (MQ=255) gAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCTTAACGCCGATACCACATTCCATAc < 1:1954282/68‑1 (MQ=255) tACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGtt > 1:726696/1‑69 (MQ=255) tACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGtt > 1:674754/1‑69 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:2821197/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:2988528/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:2533130/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:3101921/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:2500775/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:3200294/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:332502/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:473822/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:530922/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:987725/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:1542625/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:2190876/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:1565232/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:2267395/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:1470454/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:1387818/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:1746754/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:178863/69‑1 (MQ=255) aCTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:2000409/69‑1 (MQ=255) aCTTCGATCACATCCCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:2393988/69‑1 (MQ=255) cTTCGATCACATCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:2005307/68‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:569244/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:1368409/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:585841/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:3376367/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:60997/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:1039003/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:3346072/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:1456398/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:1027714/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:779542/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:935480/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:1470483/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:3092371/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:1607305/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:2494833/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:3002968/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:2169221/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:1751297/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:2097772/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:257881/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:1904166/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:1541/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:2722779/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:2600964/69‑1 (MQ=255) acaTCGCCAGTGCGGACATCGTTGTAGTTCATAACGCCGATACCACATTCCATACCGTTACGGACTTCg < 1:2597297/69‑1 (MQ=255) cAGTGCGGACGTCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGt < 1:3323029/69‑1 (MQ=255) cAGTGCGGACGTCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGt < 1:1215079/69‑1 (MQ=255) aGTGCGGACATCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTa < 1:528803/52‑1 (MQ=255) gtcgtTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCg > 1:686421/1‑52 (MQ=255) gtcgtTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGaa > 1:1190524/1‑69 (MQ=255) gtcgtTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGaa > 1:385864/1‑69 (MQ=255) gtcgtTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGaa > 1:3381265/1‑69 (MQ=255) gtcgtTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGaa > 1:1015522/1‑69 (MQ=255) gtcgtTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGaa > 1:1612422/1‑69 (MQ=255) gtcgtTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGaa > 1:982008/1‑69 (MQ=255) gtcgtTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGaa > 1:1011594/1‑69 (MQ=255) gtcgtT‑TAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAg > 1:1618015/1‑69 (MQ=255) | ATGGTACGTTGGATCTCGATGATTTCGAATACTTCGATCACATCGCCAGTGCGGACGTCGTTGTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAG > minE/2138442‑2138567 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |