| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,138,570 | G→T | 36.9% | R846R (CGC→CGA) | infB ← | fused protein chain initiation factor 2, IF2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,138,570 | 0 | G | T | 36.9% | 109.8 / 86.5 | 117 | R846R (CGC→CGA) | infB | fused protein chain initiation factor 2, IF2 |
| Reads supporting (aligned to +/- strand): ref base G (52/21); new base T (26/17); total (78/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 125 total aligned reads displayed.
GTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCACGCAGAACGCGGATCGGGT > minE/2138504‑2138631 | gTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAgcgg > 1:1301435/1‑67 (MQ=255)gTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAgcgg > 1:1622233/1‑67 (MQ=255)gTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAgcgg > 1:773713/1‑67 (MQ=255)gTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAgcgg > 1:1935260/1‑67 (MQ=255)gTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAgcgg > 1:2674927/1‑67 (MQ=255)gTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAgcgg > 1:2908880/1‑67 (MQ=255)gTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAgcgg > 1:2969503/1‑67 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGa > 1:2721970/1‑69 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGa > 1:3188532/1‑69 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGa > 1:344690/1‑69 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGa > 1:2212869/1‑69 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGa > 1:646890/1‑69 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGa > 1:705257/1‑69 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGa > 1:1910866/1‑69 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGa > 1:2980434/1‑69 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGa > 1:1774233/1‑69 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGa > 1:1763600/1‑69 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGa > 1:1695797/1‑69 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGa > 1:3159096/1‑69 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGa > 1:1012170/1‑69 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGa > 1:1150768/1‑69 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGa > 1:1234798/1‑69 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGa > 1:781937/1‑69 (MQ=255) cTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCCGCGCAGGGa > 1:764491/1‑69 (MQ=255) tACCTCATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGc > 1:1922849/1‑69 (MQ=255) tACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGc > 1:1020774/1‑69 (MQ=255) tACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGc > 1:3140238/1‑69 (MQ=255) tACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGc > 1:1080994/1‑69 (MQ=255) tACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGc > 1:29691/1‑69 (MQ=255) tACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGc > 1:2828114/1‑69 (MQ=255) tACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGc > 1:2633945/1‑69 (MQ=255) tACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGc > 1:2485478/1‑69 (MQ=255) tACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGc > 1:1537690/1‑69 (MQ=255) aCCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGcc > 1:3381889/1‑69 (MQ=255) aCCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGcc > 1:1743850/1‑69 (MQ=255) aCCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGcc > 1:3101717/1‑69 (MQ=255) aCCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGcc > 1:1511637/1‑69 (MQ=255) aCCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGcc > 1:144741/1‑69 (MQ=255) aCCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGcc < 1:1233942/69‑1 (MQ=255) aCCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGcc > 1:2011224/1‑69 (MQ=255) aCCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGcc < 1:593126/69‑1 (MQ=255) aCCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGcc < 1:3420365/69‑1 (MQ=255) aCCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGcc < 1:2118675/69‑1 (MQ=255) aCCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGcc > 1:592005/1‑69 (MQ=255) aCCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGcc > 1:419828/1‑69 (MQ=255) aCCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGcc > 1:526235/1‑69 (MQ=255) aTTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAgg > 1:2474115/1‑50 (MQ=255) aTTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAgg > 1:776343/1‑50 (MQ=255) aTTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAgg > 1:2518782/1‑50 (MQ=255) aTTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAgg > 1:368365/1‑50 (MQ=255) aTTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAgg > 1:3147919/1‑50 (MQ=255) aTTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAgg > 1:586512/1‑50 (MQ=255) aTTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAgg > 1:144289/1‑50 (MQ=255) aCCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCa < 1:3201610/41‑1 (MQ=255) aCCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAAc < 1:2332220/69‑1 (MQ=255) aCCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAAc < 1:3359205/69‑1 (MQ=255) aCCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAAc < 1:2865235/69‑1 (MQ=255) aCCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGCACTCCAGCTCGCCTTCGTAGATAAc < 1:141821/69‑1 (MQ=255) ccGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCTCCTTCGTAGAt < 1:2474083/65‑1 (MQ=255) ccGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGAt < 1:1845451/65‑1 (MQ=255) ccGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAAcc > 1:464676/1‑69 (MQ=255) ccGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAAcc > 1:2721310/1‑69 (MQ=255) ccGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAAcc > 1:2238650/1‑69 (MQ=255) ccGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAAc < 1:661542/68‑1 (MQ=255) aCGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGtt < 1:866664/69‑1 (MQ=255) aCGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGtt < 1:3297159/69‑1 (MQ=255) aCGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGtt < 1:93447/69‑1 (MQ=255) aCGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGtt < 1:355033/69‑1 (MQ=255) aCGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGtt < 1:2736601/69‑1 (MQ=255) aCGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGtt < 1:1287508/69‑1 (MQ=255) aCGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGtt < 1:2306879/69‑1 (MQ=255) aCGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGtt < 1:2448043/69‑1 (MQ=255) aCGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGtt < 1:2307711/69‑1 (MQ=255) aCGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGtt < 1:2436394/69‑1 (MQ=255) aCGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGtt < 1:1590177/69‑1 (MQ=255) aCGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGtt < 1:2040608/69‑1 (MQ=255) cGGACTTCGTTAACGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGtt < 1:2170367/68‑1 (MQ=255) tAACGGCATCTTTGAAGCGTCGCAGGGACTCCAGCGCGCCTTCGTAGATAACCACGtt < 1:892531/58‑1 (MQ=38) aCGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCACg > 1:9215/1‑62 (MQ=255) aCGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCACGCATAAcg > 1:595548/1‑69 (MQ=255) aCGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCACGCAGAAcg > 1:627037/1‑69 (MQ=255) aCGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCACGCAGAAcg > 1:2096900/1‑69 (MQ=255) aCGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCACGCAGAAcg > 1:2442389/1‑69 (MQ=255) aCGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCACGCAGAAcg > 1:2606958/1‑69 (MQ=255) aCGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCACGCAGAAcg > 1:1310843/1‑69 (MQ=255) cGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAAcc > 1:1453399/1‑50 (MQ=255) cGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCGCGCAGAAcg > 1:1955833/1‑68 (MQ=255) cGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCGCGCAGAAcg > 1:1194903/1‑68 (MQ=255) cGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCGCGCAGAAcg > 1:1119502/1‑68 (MQ=255) cGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCGCGCAGAAcg > 1:2193112/1‑68 (MQ=255) cGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCGCGCAGAAcg > 1:450255/1‑68 (MQ=255) cGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCGCGCAGAAcg > 1:2655055/1‑68 (MQ=255) cGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCGCGCAGAAcg > 1:2717877/1‑68 (MQ=255) cGTCATCTTTGAAGCGTCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCGCGCAGAAcg > 1:3227531/1‑68 (MQ=255) gTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCACGCAGAAcgcg < 1:3240712/69‑1 (MQ=255) gTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCACGCAGAAcgcg < 1:2964923/69‑1 (MQ=255) cTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGAt < 1:2318692/40‑1 (MQ=255) aaGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCACGCAGAACGCGGATCGGGt < 1:2413864/67‑1 (MQ=255) aaGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCACGCAGAACGCGGATCGGGt < 1:2840490/67‑1 (MQ=255) aaGCGCCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCACGCAGAACGCGGATCGGGt < 1:3361682/67‑1 (MQ=255) | GTAGTTCTTAACGCCGATACCACATTCCATACCGTTACGGACTTCGTTAACGTCATCTTTGAAGCGGCGCAGGGACTCCAGCTCGCCTTCGTAGATAACCACGTTGTCACGCAGAACGCGGATCGGGT > minE/2138504‑2138631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |