| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,139,470 | G→A | 58.1% | D546D (GAC→GAT) | infB ← | fused protein chain initiation factor 2, IF2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,139,470 | 0 | G | A | 58.1% | 21.5 / 54.5 | 74 | D546D (GAC→GAT) | infB | fused protein chain initiation factor 2, IF2 |
| Reads supporting (aligned to +/- strand): ref base G (6/25); new base A (13/30); total (19/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTC > minE/2139416‑2139529 | cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATc < 1:2405090/69‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATc < 1:642440/69‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATc < 1:1500168/69‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATc < 1:3086056/69‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATc < 1:3027149/69‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCAt < 1:545384/68‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCAt < 1:24358/68‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCAt < 1:2340034/68‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCAt < 1:2238373/68‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCAt < 1:2146302/68‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCAt < 1:2025024/68‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCAt < 1:2949073/68‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCAt < 1:1892968/68‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCAt < 1:1819630/68‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCAt < 1:1729242/68‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCAt < 1:3083853/68‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCAt < 1:492093/68‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCAt < 1:3418183/68‑1 (MQ=255)cATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGAATCCAGCAGTTCAt < 1:615467/68‑1 (MQ=255)cATACCTTTACGTACAGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATc < 1:512725/69‑1 (MQ=255) ccTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGAt > 1:3047362/1‑68 (MQ=255) ccTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATa > 1:3305435/1‑69 (MQ=255) cGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCAt < 1:2876640/58‑1 (MQ=255) gTACCGCTTTCAGCTCCAGTACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:707039/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:1049474/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:2866393/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:313428/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:2458758/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:380722/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:40694/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:3011418/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:572305/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:1312670/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:1599542/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:791962/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:174762/68‑1 (MQ=255) tACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:1634696/67‑1 (MQ=255) aCCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATc < 1:1934783/56‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:1567664/62‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:120192/62‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:3083444/62‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:3248910/62‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:3007097/62‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:194449/62‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:155941/62‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:2099723/62‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:2568221/62‑1 (MQ=255) gCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:670976/61‑1 (MQ=255) gCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCt > 1:1471742/1‑69 (MQ=255) gCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCt > 1:1980930/1‑69 (MQ=255) tttCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCAt < 1:180394/50‑1 (MQ=255) ttCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCttt > 1:706850/1‑68 (MQ=255) ttCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCttt > 1:856666/1‑68 (MQ=255) aGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCg < 1:950598/67‑1 (MQ=255) aGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCg < 1:1568392/67‑1 (MQ=255) ttCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:637686/44‑1 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTgg > 1:580478/1‑67 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGc > 1:3318041/1‑68 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:792330/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:2887950/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:571249/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:3179133/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:2383937/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:2348868/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:2196697/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:1822065/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:1740267/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:1536157/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:1529497/1‑69 (MQ=255) cagGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc < 1:3248211/69‑1 (MQ=255) cagGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc < 1:873639/69‑1 (MQ=255) cagGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc < 1:942385/69‑1 (MQ=255) cagGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc < 1:1216469/69‑1 (MQ=255) cagGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc < 1:970476/69‑1 (MQ=255) | CATACCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTC > minE/2139416‑2139529 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |