| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,139,485 | G→A | 35.3% | I541I (ATC→ATT) | infB ← | fused protein chain initiation factor 2, IF2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,139,485 | 0 | G | A | 35.3% | 55.7 / 44.3 | 79 | I541I (ATC→ATT) | infB | fused protein chain initiation factor 2, IF2 |
| Reads supporting (aligned to +/- strand): ref base G (19/32); new base A (14/14); total (33/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAG > minE/2139420‑2139550 | ccTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGAt > 1:3047362/1‑68 (MQ=255)ccTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATa > 1:3305435/1‑69 (MQ=255) gTACCGCTTTCAGCTCCAGTACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:707039/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:791962/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:2458758/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:572305/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:40694/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:2866393/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:3011418/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:174762/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:313428/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:1599542/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:380722/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:1312670/68‑1 (MQ=255) gTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:1049474/68‑1 (MQ=255) tACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCATCCAGCAGTTCATCAATACCGGTa < 1:1634696/67‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:1567664/62‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:155941/62‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:3083444/62‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:3248910/62‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:194449/62‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:2568221/62‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:120192/62‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:3007097/62‑1 (MQ=255) cGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:2099723/62‑1 (MQ=255) gCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:670976/61‑1 (MQ=255) gCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCt > 1:1471742/1‑69 (MQ=255) gCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCt > 1:1980930/1‑69 (MQ=255) ttCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCttt > 1:856666/1‑68 (MQ=255) ttCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCttt > 1:706850/1‑68 (MQ=255) aGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCg < 1:1568392/67‑1 (MQ=255) aGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCg < 1:950598/67‑1 (MQ=255) ttCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCgg < 1:637686/44‑1 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTgg > 1:580478/1‑67 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGc > 1:3318041/1‑68 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:2383937/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:1529497/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:1536157/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:3179133/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:1740267/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:1822065/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:792330/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:2887950/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:571249/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:2196697/1‑69 (MQ=255) cagcagGATAGCATCCAGCAGTTCATCAATACCGGTACCCGCTTTTGCAGATACGTGTACGAACTGGCt > 1:2348868/1‑69 (MQ=255) cagGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc < 1:942385/69‑1 (MQ=255) cagGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc < 1:970476/69‑1 (MQ=255) cagGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc < 1:3248211/69‑1 (MQ=255) cagGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc < 1:873639/69‑1 (MQ=255) cagGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc < 1:1216469/69‑1 (MQ=255) tCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCTCCCCAc < 1:7927/69‑1 (MQ=255) tCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCAc < 1:621142/69‑1 (MQ=255) tCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCAc < 1:105333/69‑1 (MQ=255) tCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCAc < 1:2864560/69‑1 (MQ=255) tCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCAc < 1:79391/69‑1 (MQ=255) tCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCAc < 1:2128405/69‑1 (MQ=255) tCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCAc < 1:962867/69‑1 (MQ=255) tCCAGCAGTTCATCGATACAGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCAc < 1:752179/69‑1 (MQ=255) cagcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc > 1:369260/1‑57 (MQ=255) cagcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc > 1:2570785/1‑57 (MQ=255) cagcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc > 1:2081503/1‑57 (MQ=255) cATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc > 1:702544/1‑49 (MQ=255) cATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc > 1:978482/1‑49 (MQ=255) cATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc > 1:376783/1‑49 (MQ=255) cATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc > 1:3388946/1‑49 (MQ=255) cATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc > 1:3190124/1‑49 (MQ=255) cATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc > 1:2946090/1‑49 (MQ=255) cATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc > 1:2475791/1‑49 (MQ=255) cATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTc > 1:198754/1‑49 (MQ=255) cATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGc > 1:27755/1‑68 (MQ=255) cATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGc > 1:180734/1‑68 (MQ=255) cATCAATACCGGTACCAGCTTTCGCAGATACGTGTACGAACTGGCTTTc > 1:1632884/1‑49 (MQ=39) tCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAg < 1:580714/68‑1 (MQ=255) tCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAg < 1:2907632/68‑1 (MQ=255) tCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAg < 1:2578427/68‑1 (MQ=255) tCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAg < 1:1985149/68‑1 (MQ=255) tCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAg < 1:1222482/68‑1 (MQ=255) tCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCATCCGGCAg < 1:3111268/68‑1 (MQ=255) | CCTTTACGTACCGCTTTCAGCTCCAGAACTTCCGCCTGCAGCAGGATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAG > minE/2139420‑2139550 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |