| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,139,593 | A→G | 62.0% | A505A (GCT→GCC) | infB ← | fused protein chain initiation factor 2, IF2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,139,593 | 0 | A | G | 62.0% | 38.8 / 43.8 | 71 | A505A (GCT→GCC) | infB | fused protein chain initiation factor 2, IF2 |
| Reads supporting (aligned to +/- strand): ref base A (19/8); new base G (42/2); total (61/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.01e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGG > minE/2139539‑2139659 | ctctTCTGGCAGGATGCCGTACTGGGATAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:671130/1‑62 (MQ=255)ctctTCTGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGTATCGGCTTCTg > 1:3075920/1‑62 (MQ=255)ctctTCTGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:1149434/1‑62 (MQ=255)ctctTCTGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:551874/1‑62 (MQ=255)ctctTCTGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:414340/1‑62 (MQ=255)ctctTCTGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:299941/1‑62 (MQ=255)ctctTCTGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:2957182/1‑62 (MQ=255)ctctTCTGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:2567106/1‑62 (MQ=255)ctctTCTGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:2545741/1‑62 (MQ=255)ctctTCTGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:2475729/1‑62 (MQ=255)ctctTCTGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:2388540/1‑62 (MQ=255)ctctTCTGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:2350847/1‑62 (MQ=255)ctctTCTGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:2234405/1‑62 (MQ=255)ctctTCTGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:2019483/1‑62 (MQ=255)ctctTCTGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:1817607/1‑62 (MQ=255)ctctTCTGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:1395733/1‑62 (MQ=255)ctctTCTGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:1273502/1‑62 (MQ=255)ctctTCTGGCAGGATGCCCTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:3298029/1‑62 (MQ=255)ctctTCTGGCAGGATACCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTg > 1:1579828/1‑62 (MQ=255) ctTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGa > 1:2066641/1‑69 (MQ=255) ctTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGa > 1:2062624/1‑69 (MQ=255) cAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGtt < 1:2476966/69‑1 (MQ=255) cAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGtt < 1:2429523/69‑1 (MQ=255) cAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGtt < 1:2199806/69‑1 (MQ=255) cAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGtt < 1:610696/69‑1 (MQ=255) cAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGtt < 1:2860309/69‑1 (MQ=255) cAGGATGCCGTACTGGGAGAGTTCGTTCATAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGtt < 1:3348275/69‑1 (MQ=255) aGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGtt < 1:384372/68‑1 (MQ=255) gCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAg > 1:432381/1‑41 (MQ=255) gCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTTTCGATCTTGTTCACTGc > 1:2112661/1‑69 (MQ=255) gCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTg > 1:399036/1‑68 (MQ=255) gCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGc > 1:2882583/1‑69 (MQ=255) gCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGc > 1:1064760/1‑69 (MQ=255) gCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGc > 1:2928264/1‑69 (MQ=255) cTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAaccac < 1:2781949/69‑1 (MQ=255) cTGGGAGAGTTCGTTCTTAACGCGATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAaccac < 1:1674802/69‑1 (MQ=255) cTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTgcgc > 1:1223523/1‑69 (MQ=255) cTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTgcg > 1:761335/1‑68 (MQ=255) cTTAACACGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTgcgc > 1:1829153/1‑69 (MQ=255) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCacc > 1:1645108/1‑43 (MQ=37) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:3088553/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:1198228/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:3289092/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:2858213/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:569458/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:629618/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:619088/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:1262587/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:853464/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:2899556/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:883173/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:895180/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:1290037/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:1290749/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:2498405/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:1312317/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:1371726/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:1447760/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:1862369/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:1727225/1‑45 (MQ=38) gcgATCCGGATCGGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:978494/1‑45 (MQ=38) gcgATCCGGAT‑GGCTTCTGGTTTATCGATTTTGTTCACAGCAac > 1:1421889/1‑44 (MQ=25) cgATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccacca > 1:2911087/1‑49 (MQ=255) cgATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTc > 1:3336187/1‑69 (MQ=255) cgATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTc > 1:2818086/1‑69 (MQ=255) cgATCCGGATCAGCTTCTGGTTTATCGATCTTATTCACTGCAACCACCACCGGTACCTGCGCCGCTTTc > 1:1175369/1‑69 (MQ=255) ccGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGt < 1:736594/50‑1 (MQ=255) tCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTgg > 1:585032/1‑69 (MQ=255) tCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTgg > 1:2958566/1‑69 (MQ=255) tCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTgg > 1:1662209/1‑69 (MQ=255) tCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTg > 1:2808046/1‑68 (MQ=255) | CTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGG > minE/2139539‑2139659 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |