| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,141,978 | T→C | 27.2% | P214P (CCA→CCG) | nusA ← | transcription termination/antitermination L factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,141,978 | 0 | T | C | 27.2% | 31.0 / 10.0 | 33 | P214P (CCA→CCG) | nusA | transcription termination/antitermination L factor |
| Reads supporting (aligned to +/- strand): ref base T (9/15); new base C (7/2); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.70e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAAC > minE/2141918‑2142035 | cGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACtt > 1:2128297/1‑68 (MQ=255)cGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTc > 1:2994724/1‑69 (MQ=255)cGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTc > 1:3393471/1‑69 (MQ=255)cGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTc > 1:3219240/1‑69 (MQ=255)cGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTc > 1:2963781/1‑69 (MQ=255)cGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTc > 1:2584059/1‑69 (MQ=255)cGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTc > 1:3180463/1‑69 (MQ=255)cGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTc > 1:3145929/1‑69 (MQ=255)cGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTc > 1:1839870/1‑69 (MQ=255) gCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTc < 1:2036770/68‑1 (MQ=255) gCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTc < 1:2200000/68‑1 (MQ=255) gCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTc < 1:3058335/68‑1 (MQ=255) gCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTc < 1:1774195/68‑1 (MQ=255) gCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTc < 1:1578400/68‑1 (MQ=255) aCGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCaa < 1:319993/68‑1 (MQ=255) aCGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCaa < 1:2332024/68‑1 (MQ=255) aCGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCaa < 1:2335523/68‑1 (MQ=255) aCGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCaa < 1:1587639/68‑1 (MQ=255) aCGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCaa < 1:1065023/68‑1 (MQ=255) gaACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACgg < 1:304069/69‑1 (MQ=255) gATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTc < 1:2090222/55‑1 (MQ=255) ttCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTgg < 1:541627/68‑1 (MQ=255) ttCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTgg < 1:211708/68‑1 (MQ=255) cACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATGAGCATTTCCGGCTTGGAACGAGt < 1:1232074/69‑1 (MQ=255) cACTTCTTCGCCGATTTCCGGCTCTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGt > 1:532465/1‑69 (MQ=255) cACTTCTTCGCCGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGt > 1:2664093/1‑69 (MQ=255) cACTTCTTCGCCGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGt > 1:1839685/1‑69 (MQ=255) cACTTCTTCGCCGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGt > 1:1821389/1‑69 (MQ=255) cACTTCTTCGCCGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGt > 1:1425094/1‑69 (MQ=255) cACTTCTTCGCCGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGt > 1:391456/1‑69 (MQ=255) cACTTCTTAGCCGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGt > 1:162996/1‑69 (MQ=255) ccGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGTGACGAAc < 1:149309/66‑1 (MQ=255) ccGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGTGACGAAc < 1:3404718/66‑1 (MQ=255) | CGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAAC > minE/2141918‑2142035 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |