| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,142,026 | A→G | 19.0% | T198T (ACT→ACC) | nusA ← | transcription termination/antitermination L factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,142,026 | 0 | A | G | 19.0% | 66.1 / 8.2 | 47 | T198T (ACT→ACC) | nusA | transcription termination/antitermination L factor |
| Reads supporting (aligned to +/- strand): ref base A (23/15); new base G (7/2); total (30/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCC > minE/2141960‑2142079 | cACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATGAGCATTTCCGGCTTGGAACGAGt < 1:1232074/69‑1 (MQ=255)cACTTCTTCGCCGATTTCCGGCTCTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGt > 1:532465/1‑69 (MQ=255)cACTTCTTCGCCGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGt > 1:2664093/1‑69 (MQ=255)cACTTCTTCGCCGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGt > 1:391456/1‑69 (MQ=255)cACTTCTTCGCCGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGt > 1:1839685/1‑69 (MQ=255)cACTTCTTCGCCGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGt > 1:1821389/1‑69 (MQ=255)cACTTCTTCGCCGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGt > 1:1425094/1‑69 (MQ=255)cACTTCTTAGCCGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGt > 1:162996/1‑69 (MQ=255) ccGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGTGACGAAc < 1:149309/66‑1 (MQ=255) ccGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAGCGGGTGACGAAc < 1:3404718/66‑1 (MQ=255) cAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCt > 1:799470/1‑68 (MQ=255) tACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCg > 1:2107139/1‑69 (MQ=255) cGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTTCGCGCCACGCGCTTCCgg > 1:2908469/1‑68 (MQ=255) cGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCgg > 1:2853424/1‑68 (MQ=255) gAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCg < 1:166275/69‑1 (MQ=255) gTTCGATCAGCATTTCCTGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACgg < 1:134337/69‑1 (MQ=255) gTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACgg < 1:1464124/69‑1 (MQ=255) gTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACgg < 1:1351403/69‑1 (MQ=255) tCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACgg > 1:3162473/1‑67 (MQ=255) tCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGaa < 1:51658/69‑1 (MQ=255) tCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGaa < 1:2473515/69‑1 (MQ=255) tCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGaa < 1:2506538/69‑1 (MQ=255) tCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGaa < 1:3112166/69‑1 (MQ=255) tttCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACg > 1:606320/1‑68 (MQ=255) tCCGGCTTTGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGaa < 1:526072/57‑1 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGaa < 1:933226/57‑1 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGaa < 1:2136681/57‑1 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGaa < 1:613515/57‑1 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGaa < 1:2792313/57‑1 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGaa < 1:2631319/57‑1 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:157713/1‑68 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:3188045/1‑68 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:940143/1‑68 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:951634/1‑68 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:574549/1‑68 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:965852/1‑68 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:996342/1‑68 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:1326298/1‑68 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:1579728/1‑68 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:1645991/1‑68 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:318731/1‑68 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:293715/1‑68 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:1366900/1‑68 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:2820416/1‑68 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:1026459/1‑68 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:2415244/1‑68 (MQ=255) tCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGcc > 1:235684/1‑68 (MQ=255) | CACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCC > minE/2141960‑2142079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |