| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,143,681 | G→A | 21.7% | intergenic (‑299/‑49) | metY ← / → argG | tRNA‑Met/argininosuccinate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,143,681 | 0 | G | A | 21.7% | 204.9 / 41.7 | 142 | intergenic (‑299/‑49) | metY/argG | tRNA‑Met/argininosuccinate synthetase |
| Reads supporting (aligned to +/- strand): ref base G (36/75); new base A (16/15); total (52/90) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.94e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 150 total aligned reads displayed.
AACTCTTCCTGTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATG > minE/2143613‑2143732 | aaCTCTTCCTGTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTgag > 1:1096083/1‑69 (MQ=255)aaCTCTTCCTGTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTgag > 1:18733/1‑69 (MQ=255)aaCTCTTCCTGTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTgag > 1:1861565/1‑69 (MQ=255)aaCTCTTCCTGTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTgag > 1:2899392/1‑69 (MQ=255) tctTCCTGTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGagag > 1:473694/1‑68 (MQ=255) tGTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGa < 1:3081016/66‑1 (MQ=255) gTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGa < 1:1649173/65‑1 (MQ=255) gTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGa < 1:2691666/65‑1 (MQ=255) gTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAAt < 1:542313/68‑1 (MQ=255) gTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAAt < 1:156341/68‑1 (MQ=255) gTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAAt < 1:1108336/68‑1 (MQ=255) gTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAAt < 1:481905/68‑1 (MQ=255) tctcCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATcc > 1:1888154/1‑69 (MQ=255) tctcCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATcc > 1:2710865/1‑69 (MQ=255) tctcCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATcc > 1:1072136/1‑69 (MQ=255) tctcCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATcc > 1:401436/1‑69 (MQ=255) cGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAGTTCAGTGAGAGCGGAAATCCAgg > 1:705932/1‑68 (MQ=255) cGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAgg > 1:1923931/1‑68 (MQ=255) cGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAg > 1:2678721/1‑67 (MQ=255) cGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCAAgg > 1:3392935/1‑68 (MQ=255) atcaaaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCt < 1:957147/66‑1 (MQ=255) atcaaaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCt < 1:548843/66‑1 (MQ=255) atcaaaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCt < 1:1041355/66‑1 (MQ=255) aTAAGAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtc < 1:43162/69‑1 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAgg < 1:330860/66‑1 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAgg < 1:2250453/66‑1 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAgg < 1:4274/66‑1 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAgg < 1:974485/66‑1 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtc < 1:941510/69‑1 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtc < 1:1252001/69‑1 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtc < 1:3155121/69‑1 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtc > 1:238453/1‑69 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtc < 1:885052/69‑1 (MQ=255) aaaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtcat > 1:612885/1‑69 (MQ=255) aaaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtcat > 1:3431054/1‑69 (MQ=255) aaaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtcat > 1:2162432/1‑69 (MQ=255) aaaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtcat > 1:477850/1‑69 (MQ=255) aaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtcat < 1:2093247/68‑1 (MQ=255) aGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtcatca < 1:1521488/68‑1 (MQ=255) atTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGtt > 1:3023897/1‑69 (MQ=255) aaCTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCa < 1:1748133/68‑1 (MQ=255) aaCTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCa < 1:1041704/68‑1 (MQ=255) aaCTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCa < 1:59748/68‑1 (MQ=255) aaCTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCa < 1:16648/68‑1 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg > 1:2996512/1‑69 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg > 1:1336480/1‑69 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg > 1:3338513/1‑69 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg > 1:3400762/1‑69 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg < 1:488127/69‑1 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg > 1:2655465/1‑69 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg > 1:2126545/1‑69 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg > 1:3128060/1‑69 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg < 1:2480852/69‑1 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg < 1:64997/69‑1 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAgg > 1:1692597/1‑68 (MQ=255) atttatttTGCATAAATATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg < 1:229067/66‑1 (MQ=255) ttatttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGgtgt < 1:3241535/67‑1 (MQ=255) ttatttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGgtgt < 1:1227934/67‑1 (MQ=255) ttatttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGgtgt < 1:2665128/67‑1 (MQ=255) ttatttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGgtgt < 1:1999375/67‑1 (MQ=255) tatttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGgtgt < 1:2104773/66‑1 (MQ=255) atttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAt > 1:2257504/1‑68 (MQ=255) tttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:349670/68‑1 (MQ=255) tttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:1989189/68‑1 (MQ=255) tttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:1423214/68‑1 (MQ=255) tttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:1551278/68‑1 (MQ=255) tttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:1602484/68‑1 (MQ=255) tttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:1669844/68‑1 (MQ=255) tttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:831653/68‑1 (MQ=255) ttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtca > 1:2961848/1‑38 (MQ=255) ttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtca > 1:1460436/1‑38 (MQ=255) ttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtca > 1:2542925/1‑38 (MQ=255) ttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtca > 1:1036377/1‑38 (MQ=255) ttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:989516/67‑1 (MQ=255) ttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:1189217/67‑1 (MQ=255) gCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:2332357/68‑1 (MQ=255) cATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:498418/67‑1 (MQ=255) cATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATg < 1:85844/68‑1 (MQ=255) cATAAAAATTCAGTGAGAGCGGAAATCCAGGCGCATCATCAGTTAATTAAGCAGGGTGTTATTTTATg < 1:70875/68‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:2360149/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:978267/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:1197479/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:972012/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:1624916/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:1904523/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:2302105/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:869216/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:2445342/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:2499011/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:2502840/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:2538201/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:2668143/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:3198246/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:475175/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:3434647/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:360124/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCACGCtcatcat > 1:2322648/1‑36 (MQ=37) aaaaaTTCAGAGAAAGCAGAAATCCAGGCTCATCATCAGttaattaa > 1:560597/1‑47 (MQ=37) aaaaTTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGtt < 1:402657/40‑1 (MQ=255) aaaaTTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGtt < 1:3145104/40‑1 (MQ=255) | AACTCTTCCTGTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATG > minE/2143613‑2143732 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |