| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,143,685 | G→A | 21.5% | intergenic (‑303/‑45) | metY ← / → argG | tRNA‑Met/argininosuccinate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,143,685 | 0 | G | A | 21.5% | 208.4 / 41.4 | 143 | intergenic (‑303/‑45) | metY/argG | tRNA‑Met/argininosuccinate synthetase |
| Reads supporting (aligned to +/- strand): ref base G (35/77); new base A (16/15); total (51/92) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.53e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01 | |||||||||||
Only 100 of 143 total aligned reads displayed.
TGTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATG > minE/2143622‑2143732 | tGTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGa < 1:1041597/66‑1 (MQ=255)tGTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGa < 1:3081016/66‑1 (MQ=255) gTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGa < 1:1649173/65‑1 (MQ=255) gTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGa < 1:2691666/65‑1 (MQ=255) gTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAAt < 1:481905/68‑1 (MQ=255) gTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAAt < 1:156341/68‑1 (MQ=255) gTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAAt < 1:2576889/68‑1 (MQ=255) gTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAAt < 1:1215410/68‑1 (MQ=255) tctcCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATcc > 1:2343353/1‑69 (MQ=255) tctcCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATcc > 1:1072136/1‑69 (MQ=255) tctcCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATcc > 1:1940306/1‑69 (MQ=255) tctcCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATcc > 1:397498/1‑69 (MQ=255) tctcCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATcc > 1:2710865/1‑69 (MQ=255) cGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAgg > 1:2665950/1‑68 (MQ=255) cGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAg > 1:2678721/1‑67 (MQ=255) cGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCAAgg > 1:3392935/1‑68 (MQ=255) atcaaaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCt < 1:1041355/66‑1 (MQ=255) atcaaaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCt < 1:548843/66‑1 (MQ=255) atcaaaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCt < 1:2055812/66‑1 (MQ=255) atcaaaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCt < 1:3284269/66‑1 (MQ=255) aTAAGAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtc < 1:43162/69‑1 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAgg < 1:189336/66‑1 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAgg < 1:330860/66‑1 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAgg < 1:2964960/66‑1 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAgg < 1:2250453/66‑1 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtc < 1:1252001/69‑1 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtc > 1:238453/1‑69 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtc < 1:1183568/69‑1 (MQ=255) aTAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtc < 1:941510/69‑1 (MQ=255) aaaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtcat > 1:2162432/1‑69 (MQ=255) aaaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtcat > 1:477850/1‑69 (MQ=255) aaaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtcat < 1:477213/69‑1 (MQ=255) aaaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtcat > 1:3431054/1‑69 (MQ=255) aaaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtcat > 1:861865/1‑69 (MQ=255) aaaGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtcat < 1:2093247/68‑1 (MQ=255) aGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtcatca < 1:1521488/68‑1 (MQ=255) aGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtcatca < 1:2408293/68‑1 (MQ=255) atTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGtt > 1:3023897/1‑69 (MQ=255) aaCTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCa < 1:467313/68‑1 (MQ=255) aaCTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCa < 1:59748/68‑1 (MQ=255) aaCTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCa < 1:3138077/68‑1 (MQ=255) aaCTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCa < 1:1748133/68‑1 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg < 1:415701/69‑1 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg > 1:2655465/1‑69 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg > 1:3400762/1‑69 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg < 1:2502856/69‑1 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg < 1:488127/69‑1 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg < 1:2480852/69‑1 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg > 1:1336480/1‑69 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg > 1:3338513/1‑69 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg > 1:2126545/1‑69 (MQ=255) cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg < 1:64997/69‑1 (MQ=255) cATTTA‑TTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGgtgt < 1:1872270/69‑1 (MQ=255) atttatttTGCATAAATATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg < 1:229067/66‑1 (MQ=255) ttatttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGgtgt < 1:696822/67‑1 (MQ=255) ttatttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGgtgt < 1:1227934/67‑1 (MQ=255) tatttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGgtgt < 1:2104773/66‑1 (MQ=255) atttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAt > 1:2257504/1‑68 (MQ=255) tttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:1989189/68‑1 (MQ=255) tttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:1669844/68‑1 (MQ=255) tttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:1645211/68‑1 (MQ=255) tttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:1602484/68‑1 (MQ=255) tttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:1551278/68‑1 (MQ=255) tttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:1423214/68‑1 (MQ=255) tttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:349670/68‑1 (MQ=255) ttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtca > 1:2961848/1‑38 (MQ=255) ttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtca > 1:1460436/1‑38 (MQ=255) ttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtca > 1:1036377/1‑38 (MQ=255) ttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCtc > 1:2309992/1‑37 (MQ=255) ttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:989516/67‑1 (MQ=255) ttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:1189217/67‑1 (MQ=255) gCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:2332357/68‑1 (MQ=255) cATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:498418/67‑1 (MQ=255) cATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATg < 1:85844/68‑1 (MQ=255) cATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATg < 1:598753/68‑1 (MQ=255) cATAAAAATTCAGTGAGAGCGGAAATCCAGGCGCATCATCAGTTAATTAAGCAGGGTGTTATTTTATg < 1:70875/68‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:360124/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:978267/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:972012/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:964860/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:933761/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:869216/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:1667350/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:809168/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:1904523/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:2302105/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt < 1:545180/64‑1 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:2487628/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:2499011/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:2538201/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:2668143/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:3169472/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:3198246/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTAt > 1:3434647/1‑64 (MQ=255) aaaaaTTCAGTGAAAGCAGAAATCCAGGCTCATCATCAGTTAATTAAGAAGGGTGTTATTTTAt > 1:3059173/1‑64 (MQ=255) aaaaaTTCAGAGAAAGCAGAAATCCAGGCTCATCATCAGttaattaa > 1:560597/1‑47 (MQ=37) aaaaTTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGtt < 1:3145104/40‑1 (MQ=255) aaaaTTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGtt < 1:402657/40‑1 (MQ=255) gagaGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAggg < 1:1079327/42‑1 (MQ=255) gagCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTAttt < 1:1697/48‑1 (MQ=255) | TGTCTCCGATAAAAGATGATTAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATG > minE/2143622‑2143732 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |