| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,144,059 | T→C | 42.6% | T110T (ACT→ACC) | argG → | argininosuccinate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,144,059 | 0 | T | C | 42.6% | 12.0 / 38.9 | 54 | T110T (ACT→ACC) | argG | argininosuccinate synthetase |
| Reads supporting (aligned to +/- strand): ref base T (14/17); new base C (4/19); total (18/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.31e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCATTTCATAACACCACCGGCGGCCTGACCTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTA > minE/2143992‑2144124 | gcgcATTTCATAACACCACCGGCGGCCTGACCTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACt > 1:2087108/1‑68 (MQ=255)gcgcATTTCATAACACCACCGGCGGCCTGACCTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACt > 1:2262639/1‑68 (MQ=255)gcgcATTTCATAACACCACCGGCGGCCTGACCTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACt > 1:1321004/1‑68 (MQ=255)gcgcATTTCATAACACCACCGGCGGCCTGACCTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACt > 1:3139225/1‑68 (MQ=255) tCATAACCCCACCGGCGGCCTGACCTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCAt > 1:5895/1‑69 (MQ=255) tCATAACACCACCGGCGGCCTGACCTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTgg > 1:1809923/1‑63 (MQ=255) tCATAACACCACCGGCGGCCTGACCTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCAt > 1:3162161/1‑69 (MQ=255) tCATAACACCACCGGCGGCCTGACCTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCAt > 1:1030747/1‑69 (MQ=255) tCATAACACCACCGGCGGCCTGACCTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCAt > 1:1908741/1‑69 (MQ=255) tCATAACACCACCGGCGGCCTGACCTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCAt > 1:1483234/1‑69 (MQ=255) tCATAACACCACCGGCGGCCTGACCTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCAt > 1:820487/1‑69 (MQ=255) tCATAACACCACCGGCGGCCTGACCTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCAt > 1:1127425/1‑69 (MQ=255) cTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGTTGCGATGAaagaa < 1:1244348/67‑1 (MQ=255) cTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGTTGAaagaa < 1:1974269/67‑1 (MQ=255) cTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAaagaa < 1:1551113/67‑1 (MQ=255) cTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAaagaa < 1:773254/67‑1 (MQ=255) cTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAaagaa < 1:3009025/67‑1 (MQ=255) cTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAaagaa < 1:2652208/67‑1 (MQ=255) tCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAaagaa < 1:2230357/62‑1 (MQ=255) tCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGc > 1:3190904/1‑68 (MQ=255) tCAACACGACGCCGCTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAaag < 1:2380063/60‑1 (MQ=255) tCAACACGACGCCGCTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAaag < 1:3076906/60‑1 (MQ=255) tCAACACGACGCCGCTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAaag < 1:877111/60‑1 (MQ=255) tCAACACGACGCCGCTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAaag < 1:1624884/60‑1 (MQ=255) tCAACACGACGCCGCTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAaag < 1:2011778/60‑1 (MQ=255) tCAACACGACGCCGCTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAaag < 1:1797970/60‑1 (MQ=255) acacGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTg < 1:294338/68‑1 (MQ=255) acacGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTg < 1:2844686/68‑1 (MQ=255) acacGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTg < 1:283399/68‑1 (MQ=255) cacGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTg < 1:244073/67‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:998510/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:855328/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:141164/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:3432133/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:325636/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:1917956/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:2591391/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:2199669/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:1964643/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:2545385/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTAAATATCTggg < 1:1961665/69‑1 (MQ=255) gcTTGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:2511519/68‑1 (MQ=255) gggCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGa > 1:2068318/1‑69 (MQ=255) ggCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAtag < 1:1716358/43‑1 (MQ=25) cgcCGTGACCGGCACCATGCTGGTTGCTGCTATGAaaga > 1:1206981/1‑39 (MQ=37) cgcCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTg > 1:972206/1‑49 (MQ=255) cgcCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCt > 1:224418/1‑56 (MQ=255) cgcCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTGGGGTgctg > 1:1689584/1‑62 (MQ=255) gcCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGc < 1:2489416/69‑1 (MQ=255) gcCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGc < 1:2477991/69‑1 (MQ=255) gcCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGc < 1:2850778/69‑1 (MQ=255) gcCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGc < 1:584123/69‑1 (MQ=255) ccGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGc < 1:226024/68‑1 (MQ=255) ccGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGc < 1:1873490/68‑1 (MQ=255) aCTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACgg > 1:127178/1‑59 (MQ=255) aCTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTa > 1:406478/1‑68 (MQ=255) aCTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTa > 1:3249592/1‑68 (MQ=255) aCTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTa > 1:2177696/1‑68 (MQ=255) | GCGCATTTCATAACACCACCGGCGGCCTGACCTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTA > minE/2143992‑2144124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |