| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,144,080 | G→T | 39.0% | A117A (GCG→GCT) | argG → | argininosuccinate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,144,080 | 0 | G | T | 39.0% | 51.6 / 47.5 | 64 | A117A (GCG→GCT) | argG | argininosuccinate synthetase |
| Reads supporting (aligned to +/- strand): ref base G (19/20); new base T (4/21); total (23/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.77e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAACGATATCG > minE/2144023‑2144141 | cTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGTTGCGATGAaagaa < 1:1244348/67‑1 (MQ=255)cTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGTTGAaagaa < 1:1974269/67‑1 (MQ=255)cTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAaagaa < 1:773254/67‑1 (MQ=255)cTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAaagaa < 1:3009025/67‑1 (MQ=255)cTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAaagaa < 1:2652208/67‑1 (MQ=255)cTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAaagaa < 1:1551113/67‑1 (MQ=255) tCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAaagaa < 1:2230357/62‑1 (MQ=255) tCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGc > 1:3190904/1‑68 (MQ=255) tCAACACGACGCCGCTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAaag < 1:1624884/60‑1 (MQ=255) tCAACACGACGCCGCTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAaag < 1:2011778/60‑1 (MQ=255) tCAACACGACGCCGCTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAaag < 1:3076906/60‑1 (MQ=255) tCAACACGACGCCGCTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAaag < 1:1797970/60‑1 (MQ=255) tCAACACGACGCCGCTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAaag < 1:877111/60‑1 (MQ=255) tCAACACGACGCCGCTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAaag < 1:2380063/60‑1 (MQ=255) acacGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTg < 1:2844686/68‑1 (MQ=255) acacGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTg < 1:283399/68‑1 (MQ=255) acacGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTg < 1:294338/68‑1 (MQ=255) cacGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTg < 1:244073/67‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:1964643/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:2199669/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:2545385/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:2591391/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:325636/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:855328/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:3432133/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:141164/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:998510/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:1917956/69‑1 (MQ=255) cgcTGGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTAAATATCTggg < 1:1961665/69‑1 (MQ=255) gcTTGGCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggg < 1:2511519/68‑1 (MQ=255) gggCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGa > 1:2068318/1‑69 (MQ=255) ggCCGCGCCGTGACCGGCACCATGCTGGTTGCTGCTATGAtag < 1:1716358/43‑1 (MQ=25) cgcCGTGACCGGCACCATGCTGGTTGCTGCTATGAaaga > 1:1206981/1‑39 (MQ=37) cgcCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTg > 1:972206/1‑49 (MQ=255) cgcCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCt > 1:224418/1‑56 (MQ=255) cgcCGTGACCGGCACCATGCTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTGGGGTgctg > 1:1689584/1‑62 (MQ=255) gcCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGc < 1:2850778/69‑1 (MQ=255) gcCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGc < 1:584123/69‑1 (MQ=255) gcCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGc < 1:2477991/69‑1 (MQ=255) gcCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGc < 1:2489416/69‑1 (MQ=255) ccGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGc < 1:226024/68‑1 (MQ=255) ccGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGc < 1:1873490/68‑1 (MQ=255) aCTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACgg > 1:127178/1‑59 (MQ=255) aCTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTa > 1:406478/1‑68 (MQ=255) aCTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTa > 1:2177696/1‑68 (MQ=255) aCTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTa > 1:3249592/1‑68 (MQ=255) aTGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGaaa > 1:1182502/1‑68 (MQ=255) aTGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAAc > 1:1833979/1‑69 (MQ=255) aTGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAAc > 1:1082356/1‑69 (MQ=255) aTGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAAc > 1:1301194/1‑69 (MQ=255) aTGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAAc > 1:1829835/1‑69 (MQ=255) aTGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAAc > 1:2360237/1‑69 (MQ=255) cTGGTTGCTGCTATGAAAGAAGATGGCGTGAATATCTggt < 1:2677028/40‑2 (MQ=39) cTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAACGat > 1:2107671/1‑69 (MQ=255) cTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAACGat > 1:153060/1‑69 (MQ=255) cTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAACGat > 1:3261653/1‑69 (MQ=255) cTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAACGat > 1:2276985/1‑69 (MQ=255) cTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAACGat > 1:92747/1‑69 (MQ=255) cTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAACGat > 1:2676271/1‑69 (MQ=255) cTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAACGa > 1:1181627/1‑68 (MQ=255) ttGCTGCTATGAAAGAAGATGGCGTGAATATCTggt < 1:2232346/36‑2 (MQ=38) tgctgcGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAACGATATCg < 1:2795621/68‑1 (MQ=255) tgctgcGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAACGATATCg < 1:2302541/68‑1 (MQ=255) tgctgcGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAACGATATCg < 1:1453128/68‑1 (MQ=255) | CTATTTCAACACGACGCCGCTGGGCCGCGCCGTGACTGGTACCATGCTGGTTGCTGCGATGAAAGAAGATGGCGTGAATATCTGGGGTGACGGTAGCACCTACAAAGGAAACGATATCG > minE/2144023‑2144141 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |