| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,144,404 | C→T | 41.1% | F225F (TTC→TTT) | argG → | argininosuccinate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,144,404 | 0 | C | T | 41.1% | 26.9 / 46.6 | 68 | F225F (TTC→TTT) | argG | argininosuccinate synthetase |
| Reads supporting (aligned to +/- strand): ref base C (26/14); new base T (13/15); total (39/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
TCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGC > minE/2144347‑2144472 | tCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGagag > 1:1857933/1‑69 (MQ=255)tCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGagag > 1:18553/1‑69 (MQ=255) ccTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGAt > 1:1098975/1‑69 (MQ=255) ccTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGAt > 1:2956815/1‑69 (MQ=255) ccTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGAt > 1:3047498/1‑69 (MQ=255) ccTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGAt > 1:598575/1‑69 (MQ=255) ccTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGAt > 1:1398090/1‑69 (MQ=255) ccTCAACTCCAGCGTCAAAATCGTCAACACGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGAt > 1:894434/1‑69 (MQ=255) ccAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGc > 1:573555/1‑67 (MQ=255) ccAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGc > 1:1859772/1‑67 (MQ=255) ccAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGc > 1:806151/1‑67 (MQ=255) ccAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGc > 1:3284775/1‑67 (MQ=255) ccAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGc > 1:2267539/1‑67 (MQ=255) ccAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGc > 1:2271442/1‑67 (MQ=255) ccAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGc > 1:421001/1‑67 (MQ=255) ccAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGc > 1:3177734/1‑67 (MQ=255) ccAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGc > 1:595814/1‑67 (MQ=255) ccAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGc > 1:3173620/1‑67 (MQ=255) ccAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGc > 1:3154003/1‑67 (MQ=255) ccAGCGCCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGc > 1:3301281/1‑67 (MQ=255) caaGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGc > 1:1857319/3‑67 (MQ=255) gCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCagaag < 1:1364873/69‑1 (MQ=255) gCGTCAAAATCGTCAACCCAATTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAACTCCCGGCagaa < 1:1432165/68‑1 (MQ=255) gCGTCAAAATCGTCAACCCAATTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCagaa < 1:1372882/68‑1 (MQ=255) gCGTCAAAATCGTCAACCCAATTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCagaa < 1:2351617/68‑1 (MQ=255) gCGTCAAAATCGTCAACCCAATTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCagaa < 1:2680243/68‑1 (MQ=255) gCGTCAAAATCGTCAACCCAATTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCagaa < 1:3032854/68‑1 (MQ=255) gCGTCAAAATCGTCAACCCAATTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCagaa < 1:2819347/68‑1 (MQ=255) gCGTCAAAATCGTCAACCCAATTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCagaa < 1:1817699/68‑1 (MQ=255) gCGTCAAAATCGTCAACCCAATTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCagaa < 1:1796152/68‑1 (MQ=255) gCGTCAAAATCGTCAACCCAATTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCagaa < 1:374876/68‑1 (MQ=255) gCGTCAAAATCGTCAACCCAATTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCagaa < 1:377449/68‑1 (MQ=255) gCGTCAAAATCGTCAACCCAATTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCagaa < 1:1711002/68‑1 (MQ=255) gCGTCAAAATCGTCAACCCAATTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCagaa < 1:597003/68‑1 (MQ=255) gCGTCAAAATCGTCAACCCAATTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCagaa < 1:700208/68‑1 (MQ=255) gCGTCAAAATCGTCAACCCAATTATGGGCGTGAAGTTTTGGGAGGAGAGCGTGAAAATCCCGGCagaa < 1:620084/68‑1 (MQ=255) cGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCagaaga < 1:3191516/69‑1 (MQ=255) cGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCagaaga < 1:2281204/69‑1 (MQ=255) cGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCagaaga < 1:944363/69‑1 (MQ=255) cccGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCt < 1:1432976/68‑1 (MQ=255) cccGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCt < 1:1343529/68‑1 (MQ=255) cccAATTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCagaa < 1:589081/52‑1 (MQ=255) caaTTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCAGAAGAAGTcac > 1:964357/3‑58 (MQ=255) caaTTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCt > 1:796176/3‑66 (MQ=255) caaTTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCt > 1:1541570/3‑66 (MQ=255) caaTTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCt > 1:524333/3‑66 (MQ=255) caaTTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCt > 1:1373602/3‑66 (MQ=255) caaTTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCt > 1:1761053/3‑66 (MQ=255) caaTTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCt > 1:3353460/3‑66 (MQ=255) caaTTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCt > 1:3287628/3‑66 (MQ=255) caaTTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCt > 1:1222809/3‑66 (MQ=255) caaTTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCt > 1:2280217/3‑66 (MQ=255) caaTTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCt > 1:2609414/3‑66 (MQ=255) caaTTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCt > 1:2745004/3‑66 (MQ=255) caaTTATGGGCGTGAAGTTTTGGGATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCAAAGTACGCt > 1:2839413/3‑66 (MQ=255) ttATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAAc < 1:1180909/69‑1 (MQ=255) ttATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAAc < 1:152795/69‑1 (MQ=255) aTGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACa < 1:901397/68‑1 (MQ=255) aaaTTCTGGGATGAGAGCGTGAAGATCCCGGCagaaga < 1:2765929/38‑1 (MQ=255) aaaTTCTGGGATGAGAGCGTGAAGATCCCGGCagaaga < 1:1341315/38‑1 (MQ=255) aaaTTCTGGGATGAGAGCGTGAAGATCCCGGCagaaga < 1:1511384/38‑1 (MQ=255) aaaTTCTGGGATGAGAGCGTGAAGATCCCGGCagaaga > 1:1564080/1‑38 (MQ=255) aaaTTCTGGGATGAGAGCGTGAAGATCCCGGCagaaga < 1:533230/38‑1 (MQ=255) aaaTTCTGGGATGAGAGCGTGAAGATCCCGGCagaaga > 1:1604603/1‑38 (MQ=255) aaaTTCTGGGATGAGAGCGTGAAGATCCCGGCagaaga < 1:1759323/38‑1 (MQ=255) aaaTTCTGGGATGAGAGCGTGAAGATCCCGGCagaaga > 1:2087630/1‑38 (MQ=255) aaaTTCTGGGATGAGAGCGTGAAGATCCCGGCagaaga > 1:2653744/1‑38 (MQ=255) aaaTTCTGGGATGAGAGCGTGAAGATCCCGGCagaaga > 1:2746420/1‑38 (MQ=255) cTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGgc > 1:1545729/1‑69 (MQ=255) cTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGgc > 1:343636/1‑69 (MQ=255) cTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGgc > 1:2247629/1‑69 (MQ=255) cTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGgc > 1:1185618/1‑69 (MQ=255) cTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGgc > 1:1056292/1‑69 (MQ=255) | TCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGC > minE/2144347‑2144472 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |