| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 186,734 | C→A | 34.6% | A928A (GCC→GCA) | dnaE → | DNA polymerase III alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 186,734 | 0 | C | A | 34.6% | 74.8 / 52.4 | 75 | A928A (GCC→GCA) | dnaE | DNA polymerase III alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (38/11); new base A (7/19); total (45/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.43e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01 | |||||||||||
TAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAGGTGGTATTA > minE/186667‑186800 | tAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCg < 1:659784/69‑1 (MQ=255) acGCGAAAGCTGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTg < 1:1160730/69‑1 (MQ=255) acGCGAAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTg < 1:1069809/69‑1 (MQ=255) acGCGAAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTg < 1:560594/69‑1 (MQ=255) acGCGAAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTg < 1:343719/69‑1 (MQ=255) acGCGAAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTg < 1:2720474/69‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:1857985/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:2556696/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:1267831/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:2202044/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:2856232/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:2918423/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:2961428/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:2065326/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:2967210/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:1908746/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:3134202/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:3149931/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:3212905/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:1697520/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:3259176/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:3294557/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:710103/61‑1 (MQ=255) gAAAGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:752365/61‑1 (MQ=255) aaaGCAGAAGCCATCGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACaa < 1:1233209/60‑1 (MQ=255) gCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATcc > 1:3123573/1‑69 (MQ=255) gCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATcc > 1:2948926/1‑69 (MQ=255) cGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACAAATTGAACAAt > 1:3137980/1‑56 (MQ=255) cGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACAAATTGAACAATCCTACGCCAgtt > 1:2435829/1‑66 (MQ=255) cGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACAAATTGAACAATCCTACGCCAgtt > 1:2264665/1‑66 (MQ=255) cGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACAAATTGAACAATCCTACGCCAgtt > 1:2281890/1‑66 (MQ=255) cGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACAAATTGAACAATCCTACGCCAgtt > 1:2508208/1‑66 (MQ=255) cGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACAAATTGAACAATCCTACGCCAgtt > 1:143808/1‑66 (MQ=255) cGGTCAGGCCGATATGTTCGGCGTGCTGGCAGAAGAGCCGGAACAAATTGAACAATCCTACGCCAgtt > 1:134843/1‑66 (MQ=255) ggTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTCCGCCAGCTg > 1:1366662/1‑68 (MQ=255) ggTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTg > 1:958904/1‑68 (MQ=255) ggTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTg > 1:1854601/1‑68 (MQ=255) ttCGGCGTGCTGGCCGAAGCGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGc > 1:407098/1‑64 (MQ=255) ttCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTgcc > 1:1574307/1‑62 (MQ=255) ttCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGc > 1:2433796/1‑64 (MQ=255) ttCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGc > 1:898809/1‑64 (MQ=255) ttCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGc > 1:572156/1‑64 (MQ=255) ttCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGc > 1:1050742/1‑64 (MQ=255) ttCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGc > 1:715540/1‑64 (MQ=255) ttCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGc > 1:2876299/1‑64 (MQ=255) ttCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCgg > 1:2162003/1‑67 (MQ=255) cGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGc < 1:81495/68‑1 (MQ=255) cGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGc < 1:2227217/68‑1 (MQ=255) cGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGc < 1:1722144/68‑1 (MQ=255) ggCTGGCCGAAGAGCCGGAAAAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtgg < 1:2186353/68‑1 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:1518943/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:240231/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:812161/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:1203668/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:3278178/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:1683590/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:3252823/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:1429597/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:3154175/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:3033523/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:2257155/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:2603154/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:2760232/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:2858178/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:2862034/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:2172817/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:2087866/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:1968253/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:3033974/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:303686/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:3038092/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:3082137/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:1951091/1‑68 (MQ=255) cTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAggtggt > 1:1887841/1‑68 (MQ=255) gCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAGGTGGTATTa < 1:1848957/69‑1 (MQ=255) cGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAGGTGGTATTa < 1:1538384/67‑1 (MQ=255) cGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAGGTGGTATTa < 1:3405890/67‑1 (MQ=255) cGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAGGTGGTATTa < 1:3300119/67‑1 (MQ=255) cGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAGGTGGTATTa < 1:1497467/67‑1 (MQ=255) cGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAGGTGGTATTa < 1:317178/67‑1 (MQ=255) cGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAGGTGGTACTa < 1:763643/67‑1 (MQ=255) | TAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACAAATTGAACAATCCTACGCCAGCTGCCAACCGTGGCCGGAGCAGGTGGTATTA > minE/186667‑186800 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |