| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,146,494 | A→T | 47.9% | T71T (ACT→ACA) | yhbX ← | predicted hydrolase, inner membrane |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,146,494 | 0 | A | T | 47.9% | 16.1 / 203.7 | 169 | T71T (ACT→ACA) | yhbX | predicted hydrolase, inner membrane |
| Reads supporting (aligned to +/- strand): ref base A (73/15); new base T (48/33); total (121/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.06e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 204 total aligned reads displayed.
GCAAATCCATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTT > minE/2146426‑2146553 | gCAAATCCATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGca > 1:230717/1‑69 (MQ=255)gCAAATCCATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGca > 1:2281993/1‑69 (MQ=255)gCAAATCCATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGca > 1:1242637/1‑69 (MQ=255) ccATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGcagtcagt < 1:1428706/69‑1 (MQ=255) ccATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGcagtcagt < 1:185529/69‑1 (MQ=255) ccATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGcagtcagt < 1:3013928/69‑1 (MQ=255) ccATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGcagtcagt < 1:1896988/69‑1 (MQ=255) ccATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGcagtcagt < 1:2880283/69‑1 (MQ=255) ccATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGcagtcagt > 1:3313379/1‑69 (MQ=255) ccATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGcagtcagt < 1:2757551/69‑1 (MQ=255) ccATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGcagtcagt < 1:423640/69‑1 (MQ=255) ccATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGcagtcagt > 1:2745422/1‑69 (MQ=255) ccATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGcagtcagt < 1:143499/69‑1 (MQ=255) ccATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGcagtcagt < 1:2605111/69‑1 (MQ=255) ccATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGcagtcagt < 1:1139395/69‑1 (MQ=255) ccATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGcagtcagt < 1:2373801/69‑1 (MQ=255) ccATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGcagtcagt < 1:939895/69‑1 (MQ=255) gAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAAcaca < 1:3287025/67‑1 (MQ=255) ttGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAAt > 1:2362562/1‑65 (MQ=255) ttGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTg > 1:1742163/1‑69 (MQ=255) ttGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTg > 1:2162224/1‑69 (MQ=255) ttGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTg > 1:2113134/1‑69 (MQ=255) ttGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTg > 1:2189810/1‑69 (MQ=255) ttGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTg > 1:2724763/1‑69 (MQ=255) ttGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTg > 1:2043676/1‑69 (MQ=255) ttGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTg > 1:975835/1‑69 (MQ=255) ttGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTg > 1:1100920/1‑69 (MQ=255) ttGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTg > 1:85491/1‑69 (MQ=255) ttGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTg > 1:829383/1‑69 (MQ=255) ttGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTg > 1:511767/1‑69 (MQ=255) ttGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTg > 1:1497247/1‑69 (MQ=255) ttGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTg > 1:2988265/1‑69 (MQ=255) ttGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTg > 1:716016/1‑69 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATg > 1:284236/1‑65 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:548994/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:760546/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:2640514/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:640721/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:383767/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:2800375/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:3222565/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:2580067/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:3033681/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:1350743/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:1636808/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:1399107/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:1688605/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:1269239/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:1171965/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:178156/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:1958698/1‑68 (MQ=255) tGTACCAAAGGTACACCATGAATAGAGGCTAATACTCAAGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:1996781/1‑68 (MQ=255) tGTACCAAAGGTACACAATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTgt > 1:635408/1‑68 (MQ=255) cacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAg > 1:398105/1‑68 (MQ=255) cacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAg > 1:951192/1‑68 (MQ=255) cacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAg > 1:3082904/1‑68 (MQ=255) cacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAg > 1:2529056/1‑68 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:16093/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:439815/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:3371384/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:2378003/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:2417223/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:3206036/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:1020527/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:2608222/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:2219881/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:2694404/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:602071/69‑1 (MQ=255) aTGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:2085267/68‑1 (MQ=255) tGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:1069636/67‑1 (MQ=255) tGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:358464/67‑1 (MQ=255) gAATAGAGCCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:3133533/66‑1 (MQ=255) gagGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGcaaca < 1:2237879/52‑1 (MQ=255) gagGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCgg < 1:464358/66‑1 (MQ=255) cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGAtata > 1:66699/1‑69 (MQ=255) cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGAtata > 1:615943/1‑69 (MQ=255) cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGAtata > 1:92048/1‑69 (MQ=255) cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGAtata > 1:528797/1‑69 (MQ=255) cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGAtata > 1:1047779/1‑69 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:587788/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:567892/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:1216846/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:489982/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:1616550/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:1654684/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga < 1:277855/63‑1 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:2209379/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:2464845/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:2357721/1‑63 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:2321606/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:1119252/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:1696632/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:3088485/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:2867859/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:2104784/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:2135814/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGCgatga > 1:2507252/1‑59 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGCAAGGGATGATATACGttt > 1:1749971/1‑68 (MQ=255) tCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:3254476/1‑68 (MQ=255) tCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:184477/1‑68 (MQ=255) | GCAAATCCATCATTGAAAGTTGTACCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTT > minE/2146426‑2146553 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |