| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,146,514 | C→T | 40.4% | A65T (GCA→ACA) | yhbX ← | predicted hydrolase, inner membrane |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,146,514 | 0 | C | T | 40.4% | 58.6 / 83.3 | 121 | A65T (GCA→ACA) | yhbX | predicted hydrolase, inner membrane |
| Reads supporting (aligned to +/- strand): ref base C (52/20); new base T (16/33); total (68/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.12e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 121 total aligned reads displayed.
CACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAG > minE/2146459‑2146578 | cacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAg > 1:2529056/1‑68 (MQ=255)cacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAg > 1:3082904/1‑68 (MQ=255)cacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAg > 1:398105/1‑68 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:1020527/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:2219881/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:2183130/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:2378003/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:2399007/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:2417223/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:2658026/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:2018708/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:2694404/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:2717908/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:1871591/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:3206036/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:3345042/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:3371384/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:1069359/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:43598/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:16093/69‑1 (MQ=255) cATGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:552824/69‑1 (MQ=255) aTGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:413566/68‑1 (MQ=255) aTGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:1769411/68‑1 (MQ=255) aTGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:661837/68‑1 (MQ=255) aTGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:536369/68‑1 (MQ=255) aTGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:2085267/68‑1 (MQ=255) tGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:358464/67‑1 (MQ=255) tGAATAGAGGCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:1069636/67‑1 (MQ=255) gAATAGAGCCTAATACTCATGTCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGa < 1:3133533/66‑1 (MQ=255) gagGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGcaaca < 1:2368692/52‑1 (MQ=255) gagGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGcaaca < 1:2237879/52‑1 (MQ=255) gagGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCgg < 1:464358/66‑1 (MQ=255) ggCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAgg < 1:2608015/68‑1 (MQ=255) tAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgat > 1:1629490/1‑68 (MQ=255) cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGAtata > 1:528797/1‑69 (MQ=255) cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGAtata > 1:615943/1‑69 (MQ=255) cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGAtata > 1:92048/1‑69 (MQ=255) cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGAtata > 1:2232152/1‑69 (MQ=255) cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGAtata > 1:1721018/1‑69 (MQ=255) cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGAtata > 1:1047779/1‑69 (MQ=255) cATGTCTGCCGGAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:1488628/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCa > 1:384563/1‑44 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:587788/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:1654684/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:567892/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga < 1:277855/63‑1 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:115812/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:2984872/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:1216846/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:3074697/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:3087350/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:489982/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:479347/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:1745339/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:1616550/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:2601088/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:615980/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:2357721/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:635711/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:2464845/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:2361720/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:2111033/1‑63 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatg > 1:2834796/1‑62 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGAGAAGCGGAAGGgatga < 1:207476/63‑1 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:1119252/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:2321606/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:1696632/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:4595/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:92034/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:2135814/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:3088485/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:1909065/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:2104784/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:2867859/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:609289/1‑68 (MQ=255) tCTGCCGCTGTCAGTAACACAAATGGTGTGGCAACAAGCAGGCGAAGCGCAAGGGATGATATACGttt > 1:1749971/1‑68 (MQ=255) tCTGCCGCTGTCACTAACACAAATGGTGTGGCAACAAGCAgg > 1:3139018/1‑42 (MQ=37) tCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:798238/1‑68 (MQ=255) tCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:2256936/1‑68 (MQ=255) tCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:3254476/1‑68 (MQ=255) tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACACCCAGGATg > 1:2658700/1‑67 (MQ=255) tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATg > 1:342767/1‑67 (MQ=255) tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATg > 1:2221965/1‑67 (MQ=255) tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATg > 1:2479467/1‑67 (MQ=255) tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATg > 1:2527338/1‑67 (MQ=255) tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATg > 1:2037106/1‑67 (MQ=255) tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATg > 1:1967253/1‑67 (MQ=255) tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATg > 1:3058348/1‑67 (MQ=255) tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATg > 1:3175816/1‑67 (MQ=255) tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATg > 1:1643301/1‑67 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCTAAGCGGAAGGGATGATATACGTTTTACAACCAg < 1:214154/61‑1 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAg < 1:2969852/61‑1 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAg < 1:1332237/61‑1 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAg < 1:2008957/61‑1 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAg < 1:480151/61‑1 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAg < 1:3389921/61‑1 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAg < 1:805482/61‑1 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAg < 1:1009386/61‑1 (MQ=255) acaAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAg < 1:2538568/59‑1 (MQ=255) ggTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACgaag < 1:1862325/69‑1 (MQ=255) | CACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAG > minE/2146459‑2146578 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |