| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,151,144 | G→A | 51.9% | A295A (GCC→GCT) | ftsH ← | protease, ATP‑dependent zinc‑metallo |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,151,144 | 0 | G | A | 51.9% | ‑1.3 / 70.9 | 79 | A295A (GCC→GCT) | ftsH | protease, ATP‑dependent zinc‑metallo |
| Reads supporting (aligned to +/- strand): ref base G (5/33); new base A (21/20); total (26/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.45e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCGAAACGGCCAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGT > minE/2151087‑2151209 | gTCGAAACGGCCAGGACGCTGCAGGGCGGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACgat < 1:3242589/69‑1 (MQ=255)gTCGAAACGGCCAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACgat < 1:2646747/69‑1 (MQ=255)gTCGAAACGGCCAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACgat < 1:804876/69‑1 (MQ=255)gTCGAAACGGCCAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACgat < 1:2143470/69‑1 (MQ=255)gTCGAAACGGCCAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACgat < 1:1968894/69‑1 (MQ=255) tCGAAACGGCCAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACgat < 1:2689507/68‑1 (MQ=255) aCGGCCAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACgatgat < 1:272643/66‑1 (MQ=255) aCGGCCAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACgatgat < 1:800242/66‑1 (MQ=255) aCGGCCAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACgatgat < 1:1744842/66‑1 (MQ=255) cAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGt < 1:2614649/69‑1 (MQ=255) cAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGt < 1:273054/69‑1 (MQ=255) cAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGt < 1:2835496/69‑1 (MQ=255) cAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGt < 1:300962/69‑1 (MQ=255) cAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGt < 1:2112300/69‑1 (MQ=255) cAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGt < 1:3322638/69‑1 (MQ=255) cAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGt < 1:1323785/69‑1 (MQ=255) aGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGt < 1:2200323/68‑1 (MQ=255) gACGCAGCAGGGCGGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATTATACCTTCGTTAc < 1:136941/69‑1 (MQ=255) gACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTAc < 1:2423278/69‑1 (MQ=255) gACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTAc < 1:139755/69‑1 (MQ=255) gACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTAc < 1:2130268/69‑1 (MQ=255) gACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTAc < 1:1427180/69‑1 (MQ=255) gACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTAc < 1:2877698/69‑1 (MQ=255) gACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTAc < 1:1126349/69‑1 (MQ=255) gACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTAc < 1:3053490/69‑1 (MQ=255) gACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTAc < 1:1886916/69‑1 (MQ=255) gACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTAc < 1:1824979/69‑1 (MQ=255) gACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTAc < 1:1688755/69‑1 (MQ=255) gACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTAc < 1:1795909/69‑1 (MQ=255) gACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTAc < 1:3363403/69‑1 (MQ=255) gACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTAc < 1:2468513/69‑1 (MQ=255) aCGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTAc < 1:857692/68‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:98417/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:939091/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:1107435/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:2618203/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:492574/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:3027200/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:3036588/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:775725/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:312784/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:623572/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:3373111/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:3420295/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:241984/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:1814686/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:1514390/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:2033551/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:2081177/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:1325717/54‑1 (MQ=255) agcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:113039/54‑1 (MQ=255) gcagGGCTGGGTCGAGAACGTCCGGACGGTTAGTCGCAGCGATAACGATGATa < 1:2413949/53‑1 (MQ=255) agGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTACCTTCGaa > 1:2841183/1‑68 (MQ=255) agGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTACCTTCGaa > 1:219704/1‑68 (MQ=255) agGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTACCTTCGaa > 1:88011/1‑68 (MQ=255) agGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTACCTTCGaa > 1:1945970/1‑68 (MQ=255) tCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTACCTTCGAAGccatccat > 1:3138939/1‑68 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:855419/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1164299/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1052954/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:787225/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1502162/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1747011/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1750831/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:9883/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1751417/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1769921/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:3320813/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1835606/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:3237479/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:3188291/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1966813/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:2182857/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:2246261/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:2735019/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:2381855/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:2717140/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTAACTTCAAAACCATCCATCTCAAcc > 1:723580/1‑69 (MQ=255) cGGTTAGTCGCGGCGATAACGATGATACCTTCGTTAc < 1:2445456/37‑1 (MQ=255) cgcgGCGATAACGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGt > 1:1132630/1‑69 (MQ=255) cgcgGCGATAACGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGt > 1:2283953/1‑69 (MQ=255) | GTCGAAACGGCCAGGACGCAGCAGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGT > minE/2151087‑2151209 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |