| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,151,174 | G→A | 48.6% | F285F (TTC→TTT) | ftsH ← | protease, ATP‑dependent zinc‑metallo |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,151,174 | 0 | G | A | 48.6% | 2.9 / 66.1 | 76 | F285F (TTC→TTT) | ftsH | protease, ATP‑dependent zinc‑metallo |
| Reads supporting (aligned to +/- strand): ref base G (34/5); new base A (21/16); total (55/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.00e-01 | |||||||||||
AGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGACCACCGCCCAG > minE/2151109‑2151239 | agGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTACCTTCGaa > 1:88011/1‑68 (MQ=255)agGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTACCTTCGaa > 1:219704/1‑68 (MQ=255)agGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTACCTTCGaa > 1:2841183/1‑68 (MQ=255)agGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTACCTTCGaa > 1:1945970/1‑68 (MQ=255) tCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTACCTTCGAAGccatccat > 1:3138939/1‑68 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1052954/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:2717140/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:2381855/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:2735019/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:2246261/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:2182857/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1966813/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:3188291/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:3237479/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:3320813/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1769921/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1164299/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1751417/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1750831/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1747011/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:855419/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1502162/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:9883/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:1835606/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAAcc > 1:787225/1‑69 (MQ=255) aCGTCCGGACGGTTAGTCGCAGCGATAACGATGATACCTTCGTTAACTTCAAAACCATCCATCTCAAcc > 1:723580/1‑69 (MQ=255) cgcgGCGATAACGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGt > 1:2283953/1‑69 (MQ=255) cgcgGCGATAACGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGt > 1:1132630/1‑69 (MQ=255) agcgATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAACCAGCATCTGGTTCAGTGTCTg < 1:514356/68‑1 (MQ=255) agcgATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAACCAGCATCTGGTTCAGAGTCTg < 1:106058/68‑1 (MQ=255) agcgATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAACCAGCATCTGGTTCAGAGTCTg < 1:2666839/68‑1 (MQ=255) agcgATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAACCAGCATCTGGTTCAGAGTCTg < 1:291634/68‑1 (MQ=255) agcgATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAACCAGCATCTGGTTCAGAGTCTg < 1:2996348/68‑1 (MQ=255) agcgATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAACCAGCATCTGGTTCAGAGTCTg < 1:3319953/68‑1 (MQ=255) agcgATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAACCAGCATCTGGTTCAGAGTCTg < 1:3391647/68‑1 (MQ=255) agcgATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAACCAGCATCTGGTTCAGAGTCTg < 1:2580542/68‑1 (MQ=255) agcgATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAACCAGCATCTGGTTCAGAGTCTg < 1:1225692/68‑1 (MQ=255) agcgATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAACCAGCATCTGGTTCAGAGTCTg < 1:128805/68‑1 (MQ=255) agcgATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAACCAGCATCTGGTTCAGAGTCTg < 1:1904903/68‑1 (MQ=255) agcgATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAACCAGCATCTGGTTCAGAGTCTg < 1:1317227/68‑1 (MQ=255) gcgATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAACCAGCATCTGGTTCAGAGTCTg < 1:286619/68‑1 (MQ=255) gcgATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAACCAGCATCTGGTTCAGAGTCTg < 1:1977346/68‑1 (MQ=255) gcgATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAACCAGCATCTGGTTCAGAGTCTg < 1:736785/68‑1 (MQ=255) gcgATAACGATGATACCTTCGTTACCTTCAAAACCATCCATCTCAACCAGCATCTGGTTCAGAGTCTg < 1:2414224/68‑1 (MQ=255) aaCGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACg > 1:742770/1‑69 (MQ=255) aaCGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACg > 1:2747977/1‑69 (MQ=255) aaCGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACg > 1:2078749/1‑69 (MQ=255) aaCGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACg > 1:1569426/1‑69 (MQ=255) aaCGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACg > 1:1865718/1‑69 (MQ=255) aaCGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACg > 1:780464/1‑69 (MQ=255) aaCGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACg > 1:3257493/1‑69 (MQ=255) ttCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCa < 1:1121647/44‑1 (MQ=255) ttCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCa < 1:647831/44‑1 (MQ=255) ttCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCa < 1:399415/44‑1 (MQ=255) ttCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCa < 1:2924486/44‑1 (MQ=255) ttCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCa < 1:1879273/44‑1 (MQ=255) ttCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGacc > 1:3229992/1‑69 (MQ=255) ttCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGacc > 1:3290172/1‑69 (MQ=255) ttCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGacc > 1:1870606/1‑69 (MQ=255) ttCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGacc > 1:3040937/1‑69 (MQ=255) ttCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGacc > 1:1841751/1‑69 (MQ=255) ttCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGacc > 1:3431740/1‑69 (MQ=255) ttCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGacc > 1:1267628/1‑69 (MQ=255) ttCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGacc > 1:290586/1‑69 (MQ=255) ttCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGac > 1:2684997/1‑68 (MQ=255) ttCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGac > 1:799119/1‑68 (MQ=255) ttCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGac > 1:2846306/1‑68 (MQ=255) tCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGacca > 1:2907735/1‑69 (MQ=255) tCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGacca > 1:3424118/1‑69 (MQ=255) tCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGacc > 1:2078202/1‑68 (MQ=255) cGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGACCACCGc > 1:671812/1‑63 (MQ=255) cGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGACCACCGCCCa > 1:2263385/1‑66 (MQ=255) cGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGACCACCGCCCAg > 1:2134256/1‑67 (MQ=255) cGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGACCACCGCCCAg > 1:2473318/1‑67 (MQ=255) cGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGACCACCGCCCAg > 1:922113/1‑67 (MQ=255) cGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGACCACCGCCCAg > 1:2641368/1‑67 (MQ=255) | AGGGCCGGGTCGAGAACGTCCGGACGGTTAGTCGCGGCGATAACGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGACCACCGCCCAG > minE/2151109‑2151239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |