| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 188,635 | A→G | 31.1% | K44K (AAA→AAG) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 188,635 | 0 | A | G | 31.1% | 81.0 / 45.6 | 93 | K44K (AAA→AAG) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (27/37); new base G (21/8); total (48/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.89e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01 | |||||||||||
GGAGTCGGCGCTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGT > minE/188569‑188692 | ggAGTCGGCGCTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAAtt > 1:3022899/1‑69 (MQ=255)ggAGTCGGCGCTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAAtt > 1:2828555/1‑69 (MQ=255)ggAGTCGGCGCTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAAtt > 1:3094352/1‑69 (MQ=255) gAGTCGGCGCTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAAttt > 1:3369370/1‑69 (MQ=255) gAGTCGGCGCTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAAttt > 1:1526875/1‑69 (MQ=255) ggCGCTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCg > 1:552187/1‑68 (MQ=255) ggCGCTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCg > 1:1811005/1‑68 (MQ=255) ggCGCTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCg > 1:1582370/1‑68 (MQ=255) ggCGCTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCg > 1:3347335/1‑68 (MQ=255) ggCGCTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCg > 1:3216328/1‑68 (MQ=255) ggCGCTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCg > 1:2793669/1‑68 (MQ=255) cgcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAg < 1:1889507/68‑1 (MQ=255) cgcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAg < 1:2039703/68‑1 (MQ=255) cgcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAg < 1:1941827/68‑1 (MQ=255) cgcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAg < 1:3083029/68‑1 (MQ=255) cgcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAg < 1:1875729/68‑1 (MQ=255) cgcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAg < 1:2948363/68‑1 (MQ=255) cgcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAg < 1:2916404/68‑1 (MQ=255) cgcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAg < 1:1072745/68‑1 (MQ=255) cgcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAg < 1:1367761/68‑1 (MQ=255) gcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGct < 1:2727434/69‑2 (MQ=255) gcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCa < 1:2712476/69‑1 (MQ=255) gcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCa < 1:2433688/69‑1 (MQ=255) gcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCa < 1:2452841/69‑1 (MQ=255) gcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCa < 1:581145/69‑1 (MQ=255) gcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCa < 1:1238479/69‑1 (MQ=255) gcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCa < 1:2592052/69‑1 (MQ=255) gcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCa < 1:519931/69‑1 (MQ=255) gcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCa < 1:1908931/69‑1 (MQ=255) gcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCa < 1:436094/69‑1 (MQ=255) gcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCa < 1:1821772/69‑1 (MQ=255) gcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCa < 1:2826549/69‑1 (MQ=255) gcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCa < 1:3399653/69‑1 (MQ=255) gcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCa < 1:106374/69‑1 (MQ=255) gcTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTCATTTGCTGAAATTTATCGAGCa < 1:3142176/69‑1 (MQ=255) cTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCa < 1:3061612/68‑1 (MQ=255) tggtggCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATa < 1:2242488/69‑1 (MQ=255) tggtggCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATa < 1:2822449/69‑1 (MQ=255) tggtggCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATa < 1:2560243/69‑1 (MQ=255) tggtggCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATa < 1:1768143/69‑1 (MQ=255) tggtggCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATa < 1:2220478/69‑1 (MQ=255) tggtggCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATa < 1:1689060/69‑1 (MQ=255) gcAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATa < 1:1186026/62‑1 (MQ=255) tCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCa < 1:1808401/51‑1 (MQ=255) aGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAGTTTATCGa < 1:1542919/46‑1 (MQ=255) aGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAGTTTATCGa < 1:1698717/46‑1 (MQ=255) aGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAGTTTATCGa < 1:825973/46‑1 (MQ=255) aGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAGTTTATCGa < 1:738585/46‑1 (MQ=255) aGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAGTTTATCGa < 1:1135678/46‑1 (MQ=255) aGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAGTTTATCGa < 1:3349821/46‑1 (MQ=255) aGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAGTTTATCGa < 1:352688/46‑1 (MQ=255) gATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAGTTTATCGa < 1:3185214/45‑1 (MQ=255) aTTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTg < 1:581533/69‑1 (MQ=255) cacaAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGa < 1:1822839/67‑1 (MQ=255) cacaAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGa < 1:239091/67‑1 (MQ=255) cacaAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGa < 1:315356/67‑1 (MQ=255) actgCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTg > 1:975134/4‑60 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:2834004/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:1088439/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:816612/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:1117978/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:1371669/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:467596/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:1628367/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:386395/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:384583/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:1695598/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:1717284/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:338060/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:1874082/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:3334123/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:2867365/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:2541918/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:2761891/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:3097318/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:2586794/1‑66 (MQ=255) aCAGCGTTGATTTGCTGAAGTTTATCGAACATAACCCACGAATTTGCGGCGTGATTTTTGACTggg > 1:2669914/1‑66 (MQ=255) cAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATg > 1:66560/1‑68 (MQ=255) gATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTg > 1:2058182/1‑52 (MQ=255) gATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTAc > 1:3338215/1‑66 (MQ=255) gATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGt > 1:1009462/1‑69 (MQ=255) gATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGt > 1:196775/1‑69 (MQ=255) gATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGt > 1:971193/1‑69 (MQ=255) gATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGt > 1:728656/1‑69 (MQ=255) gATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGt > 1:727334/1‑69 (MQ=255) gATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGt > 1:675628/1‑69 (MQ=255) gATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGt > 1:1201389/1‑69 (MQ=255) gATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGt > 1:1224180/1‑69 (MQ=255) gATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGt > 1:123173/1‑69 (MQ=255) gATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGt > 1:448399/1‑69 (MQ=255) gATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGt > 1:2295135/1‑69 (MQ=255) gATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGt > 1:211357/1‑69 (MQ=255) gATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGt > 1:2083755/1‑69 (MQ=255) | GGAGTCGGCGCTGGTGGCGCAAGGCTTTCAGATTATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGT > minE/188569‑188692 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |