| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,159,315 | C→T | 49.8% | T171T (ACC→ACT) | ispB → | octaprenyl diphosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,159,315 | 0 | C | T | 49.8% | ‑5.2 / 79.7 | 87 | T171T (ACC→ACT) | ispB | octaprenyl diphosphate synthase |
| Reads supporting (aligned to +/- strand): ref base C (22/21); new base T (23/20); total (46/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.33e-01 | |||||||||||
AACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAG > minE/2159262‑2159378 | aaCGATCCGGACATCACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGt > 1:1028377/1‑64 (MQ=255)aaCGATCCGGACATCACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAg > 1:2725862/1‑69 (MQ=255)aaCGATCCGGACATCACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAg > 1:2773112/1‑69 (MQ=255)aaCGATCCGGACATCACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAg > 1:1423846/1‑69 (MQ=255)aaCGATCCGGACATCACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAg > 1:1002856/1‑69 (MQ=255) tCCGGACATCACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTg > 1:364071/1‑68 (MQ=255) ccGGACATCACTGAAGAAAACTACATTCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:3010681/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGcc > 1:912414/1‑69 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGcc > 1:53109/1‑69 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:3311624/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:259015/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:2534863/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:3265777/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:2970614/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:2146381/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:2128005/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:311310/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:1833970/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:1803859/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:335575/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:731390/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:1401893/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:3198495/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:1780156/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:3264618/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:3410466/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGtt < 1:723548/59‑1 (MQ=255) ccGGACATCACTGAAGAAAACTACATGCGCGTTATCTACAGCAAGACTGAGCGTCTGtt < 1:1396449/59‑1 (MQ=255) ccGGACATAACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAAcc > 1:321682/1‑48 (MQ=255) aTCACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAg < 1:3055218/69‑1 (MQ=255) aTCACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAg < 1:3089133/69‑1 (MQ=255) aTCACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAg < 1:1590495/69‑1 (MQ=255) cACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTg < 1:1833737/49‑1 (MQ=255) cACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTg < 1:3429543/49‑1 (MQ=255) aCTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAg > 1:622369/1‑66 (MQ=255) gaagaaAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTcc > 1:2588689/1‑69 (MQ=255) gaagaaAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTcc > 1:1775251/1‑69 (MQ=255) gaaAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCggg > 1:2197288/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:3413234/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:2937490/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:3136097/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:3285346/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:402494/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:1124293/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:1151930/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:1232388/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:1308805/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:1333437/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:1422769/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:1433622/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:154484/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:710042/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:698735/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:1965989/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:2112398/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:2309855/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:2353837/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:469184/1‑69 (MQ=255) cTACATGCGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:2675892/1‑69 (MQ=255) cTACATGCGCGTTATCTAAAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:613303/1‑69 (MQ=255) cTACATACGCGTTATCTACAGCAAGACTGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTct > 1:441200/1‑69 (MQ=255) aCATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGc < 1:938797/46‑1 (MQ=255) tGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTctggct < 1:3121982/68‑1 (MQ=255) tGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTctggct < 1:3427795/68‑1 (MQ=255) tGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTctggct < 1:681942/68‑1 (MQ=255) tGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTctggct < 1:2389233/68‑1 (MQ=255) aTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCggg > 1:2743183/1‑51 (MQ=255) aTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGAt > 1:1226861/1‑53 (MQ=255) aTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGAt > 1:1354442/1‑53 (MQ=255) aTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGAt > 1:1719751/1‑53 (MQ=255) aTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGAt > 1:2497647/1‑53 (MQ=255) aTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGAt > 1:1926673/1‑53 (MQ=255) aTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGgctggctg < 1:2258562/65‑1 (MQ=255) aTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGgctggctg < 1:616624/65‑1 (MQ=255) aTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGgctggctg < 1:241508/65‑1 (MQ=255) aTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACg > 1:3252233/1‑69 (MQ=255) aTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACg > 1:461385/1‑69 (MQ=255) aTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACg > 1:343077/1‑69 (MQ=255) aTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACg > 1:3124867/1‑69 (MQ=255) aTCTATAGCAAAACAGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGAt > 1:2919305/1‑53 (MQ=255) aaaaCCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCggaggag < 1:2422050/69‑1 (MQ=255) aaaaCCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCggaggag < 1:692931/69‑1 (MQ=255) aaaaCCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCggaggag < 1:1915757/69‑1 (MQ=255) aaaaCCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCggaggag < 1:700840/69‑1 (MQ=255) aaaaCCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCggaggag < 1:1898724/69‑1 (MQ=255) aaaaCCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCggaggag < 1:2771319/69‑1 (MQ=255) aaaaCCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCggaggag < 1:73951/69‑1 (MQ=255) aaaaCCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCggaggag < 1:1284277/69‑1 (MQ=255) | AACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAG > minE/2159262‑2159378 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |