| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,160,046 | G→A | 21.1% | A15A (GCG→GCA) | sfsB → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,160,046 | 0 | G | A | 21.1% | 200.2 / 38.1 | 136 | A15A (GCG→GCA) | sfsB | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (54/53); new base A (26/3); total (80/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.40e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 136 total aligned reads displayed.
CTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGTTTGAGTTCCTCA > minE/2159984‑2160109 | cTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTTACATCATTGCGGGTTTg > 1:2237512/1‑69 (MQ=255)cTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGttt > 1:380406/1‑68 (MQ=255)cTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTg > 1:3421300/1‑69 (MQ=255)cTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTg > 1:2780763/1‑69 (MQ=255)cTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTg > 1:2803124/1‑69 (MQ=255)cTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTg > 1:2098428/1‑69 (MQ=255)cTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTg > 1:1837860/1‑69 (MQ=255)cTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTg > 1:1123463/1‑69 (MQ=255)cTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTg > 1:1770988/1‑69 (MQ=255)cTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTg > 1:2269195/1‑69 (MQ=255)cTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCAGCCCGCTGACATCATTGCGGGTTTg > 1:3308296/1‑69 (MQ=255) gAGTGAGGAAAAATGGAAATTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaag < 1:1441511/69‑1 (MQ=255) gAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaag < 1:675420/69‑1 (MQ=255) gAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaag < 1:744785/69‑1 (MQ=255) gAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaag < 1:2705121/69‑1 (MQ=255) gAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaag < 1:2714322/69‑1 (MQ=255) gAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaag < 1:2908664/69‑1 (MQ=255) gAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaag < 1:848611/69‑1 (MQ=255) aGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaag > 1:260502/1‑64 (MQ=255) aGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaag > 1:1921593/1‑64 (MQ=255) aGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaag > 1:2488766/1‑64 (MQ=255) aGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaag > 1:3115160/1‑64 (MQ=255) aGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCaag > 1:2059377/1‑64 (MQ=255) aGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAggg < 1:3035606/69‑1 (MQ=255) aGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAggg > 1:3171243/1‑69 (MQ=255) aGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAggg > 1:810143/1‑69 (MQ=255) aGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAggg > 1:1914375/1‑69 (MQ=255) gaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTc < 1:1961425/68‑1 (MQ=255) gaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTc < 1:265033/68‑1 (MQ=255) gaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCAGGTTTGCCCAAGAAGGGAACGTc < 1:359589/68‑1 (MQ=255) aaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTc < 1:2833779/69‑1 (MQ=255) aaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTc < 1:2946861/69‑1 (MQ=255) aaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTc < 1:818838/69‑1 (MQ=255) aaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTc < 1:960051/69‑1 (MQ=255) aaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTc < 1:1066408/69‑1 (MQ=255) aaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTc < 1:1498281/69‑1 (MQ=255) aaTGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTc < 1:1828994/69‑1 (MQ=255) aaGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATggc > 1:1683320/1‑69 (MQ=255) tGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGa < 1:1164579/69‑1 (MQ=255) tGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGa < 1:1313530/69‑1 (MQ=255) tGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGa < 1:3141885/69‑1 (MQ=255) tGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGa < 1:1384693/69‑1 (MQ=255) tGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGa < 1:1447307/69‑1 (MQ=255) tGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGa < 1:1220752/69‑1 (MQ=255) tGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGa < 1:1739455/69‑1 (MQ=255) tGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGa < 1:1096300/69‑1 (MQ=255) tGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAACGGCGGCGGAATCTCGCAGa < 1:824462/69‑1 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGTGAACGTCAATgg > 1:1365245/1‑52 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggcg > 1:1289384/1‑54 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggcg > 1:3231419/1‑54 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggcg > 1:2081736/1‑54 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggcg > 1:1436992/1‑54 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggcg > 1:3377278/1‑54 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggcg > 1:2988261/1‑54 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggcg > 1:2096699/1‑54 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggcg > 1:1833108/1‑54 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggcg > 1:34134/1‑54 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggcg > 1:2331800/1‑54 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggcg > 1:2215301/1‑54 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggcg > 1:2775350/1‑54 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggcg > 1:1216752/1‑54 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggcg > 1:547003/1‑54 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggcg > 1:924540/1‑54 (MQ=255) tGGCATCCCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggca > 1:1769539/1‑53 (MQ=255) tGGCATCACGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAAt > 1:2055760/1‑50 (MQ=255) tGGCATACCGCTGACATCATTGCAGGTTTGCGCAAGAAGGGAACGTCAATggcg > 1:2500093/1‑54 (MQ=255) cccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTc < 1:1307012/41‑1 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa > 1:732962/1‑41 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa < 1:421758/41‑1 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa < 1:428533/41‑1 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa > 1:1107128/1‑41 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa < 1:437695/41‑1 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa < 1:736293/41‑1 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa < 1:3309270/41‑1 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa < 1:3190867/41‑1 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa < 1:187227/41‑1 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa > 1:3014967/1‑41 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa < 1:2852379/41‑1 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa > 1:1805309/1‑41 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa > 1:1104619/1‑41 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa < 1:3239796/41‑1 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCa < 1:2644598/41‑1 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGt > 1:169175/1‑68 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGt > 1:2299713/1‑68 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGt > 1:2303573/1‑68 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGt > 1:49033/1‑68 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGt > 1:822098/1‑68 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGt > 1:399045/1‑68 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGt > 1:2807104/1‑68 (MQ=255) ccGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGt > 1:3159277/1‑68 (MQ=255) tGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGTTTGAg < 1:1606412/69‑1 (MQ=255) catTGCGGGTTTGCGCAAGATGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGTTTGAGTTCCTc > 1:3415222/1‑69 (MQ=255) catTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGTTTGAGTTCCTc > 1:1951066/1‑69 (MQ=255) catTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGTTTGAGTTCCTc > 1:1045920/1‑69 (MQ=255) catTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGTTTGAGTTCCTc > 1:1589995/1‑69 (MQ=255) catTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGTTTGAGTTCCTc > 1:1692869/1‑69 (MQ=255) catTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGTTTGAGTTCCTc > 1:1697374/1‑69 (MQ=255) catTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGTTTGAGTTCCTc > 1:2587074/1‑69 (MQ=255) catTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGTTTGAGTTCCTc > 1:2549570/1‑69 (MQ=255) catTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGTTTGAGTTCCTc > 1:2007333/1‑69 (MQ=255) | CTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGCGCAAGAAGGGAACGTCAATGGCGGCGGAATCTCGCAGAAATGGTTTGAGTTCCTCA > minE/2159984‑2160109 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |