| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 188,890 | A→G | 36.7% | T129T (ACA→ACG) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 188,890 | 0 | A | G | 36.7% | 49.6 / 54.2 | 87 | T129T (ACA→ACG) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (12/43); new base G (19/13); total (31/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.49e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
GGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGCA > minE/188833‑188958 | ggAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCa > 1:927220/1‑68 (MQ=255)ggAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCa > 1:818041/1‑68 (MQ=255)ggAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCa > 1:1909437/1‑68 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTAcgccgc > 1:1546676/1‑56 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:1104483/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:2395121/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:2781501/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:2250722/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:2192234/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:2144776/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:3182545/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:1942835/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:1882989/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:1876913/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:1850552/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:560633/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:964179/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:844118/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:1291254/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:809748/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:796339/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:139033/1‑69 (MQ=255) tatCGCCATTCGTATGCGTCAGTACACCAACGAATATCTTGATAACATTACGCCGCCGTTCACGAAAGc > 1:883199/1‑69 (MQ=255) tCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCt < 1:1320726/69‑1 (MQ=255) tCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCt < 1:222117/69‑1 (MQ=255) tCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCt < 1:2015466/69‑1 (MQ=255) tCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCt < 1:1224822/69‑1 (MQ=255) tCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCt < 1:2994005/69‑1 (MQ=255) cGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCt < 1:1851386/68‑1 (MQ=255) cGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCt < 1:1996966/68‑1 (MQ=255) cGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCt < 1:3020155/68‑1 (MQ=255) cGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAg > 1:41577/1‑58 (MQ=255) cGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGttt < 1:1395998/66‑1 (MQ=255) gacggATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:1584742/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGTGGAAg < 1:3089724/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGaaa < 1:3211101/69‑2 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGaa > 1:587225/1‑68 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:45281/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:468919/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:2985674/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg > 1:2922191/1‑69 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:2785703/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:1038586/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:995010/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:2678766/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:2655830/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:1702530/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:1163743/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:2555389/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:2427789/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:1218688/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:2586348/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:1239883/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:2258141/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:2085798/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:1291266/69‑1 (MQ=255) gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAg < 1:2349146/69‑1 (MQ=255) tatCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACAcc > 1:336399/1‑69 (MQ=255) tatCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACAcc > 1:2768402/1‑69 (MQ=255) tCTTGATAACATTACGCCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCtt < 1:3402768/69‑1 (MQ=255) tCTTGATAACATTACGCCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCtt < 1:3044050/69‑1 (MQ=255) tCTTGATAACATTACGCCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCtt < 1:452497/69‑1 (MQ=255) tCTTGATAACATTACGCCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCtt < 1:3141855/69‑1 (MQ=255) tCTTGATAACATTACGCCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCtt < 1:2828846/69‑1 (MQ=255) tCTTGATAACATTACGCCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCtt < 1:2269397/69‑1 (MQ=255) tCTTGATAACATTACGCCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCtt < 1:1386023/69‑1 (MQ=255) tCTTGATAACATTACGCCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCtt < 1:2786609/69‑1 (MQ=255) tCTTGATAACATTACGCCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCtt < 1:2781479/69‑1 (MQ=255) tCTTGATAACATTACGCCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCtt < 1:2724244/69‑1 (MQ=255) tCTTGATAACATTACGCCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCtt < 1:2591969/69‑1 (MQ=255) tCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCtt > 1:1659151/1‑69 (MQ=255) tCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCtt > 1:2035047/1‑69 (MQ=255) cTTGATAACATTACGCCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCtt < 1:3213092/68‑1 (MQ=255) cTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCttt > 1:1991481/1‑69 (MQ=255) cTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCttt > 1:2079379/1‑69 (MQ=255) tatcATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTg < 1:2767627/63‑1 (MQ=255) tAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTg < 1:3348254/66‑1 (MQ=255) tAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTg < 1:1146536/66‑1 (MQ=255) tAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTg < 1:3377374/66‑1 (MQ=255) tAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTg < 1:352868/66‑1 (MQ=255) tAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTg < 1:380399/66‑1 (MQ=255) tAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTg < 1:1941338/66‑1 (MQ=255) tAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTg < 1:3029377/66‑1 (MQ=255) tAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTg < 1:3005703/66‑1 (MQ=255) tAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTg < 1:921946/66‑1 (MQ=255) tAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTg < 1:2703323/66‑1 (MQ=255) tAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTg < 1:2355182/66‑1 (MQ=255) cATTACGCCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACTCCtt < 1:1196550/60‑1 (MQ=255) acacCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCgggg > 1:3359001/1‑69 (MQ=255) acacCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCgggg < 1:1511835/69‑1 (MQ=255) acacCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCgggg < 1:2115683/69‑1 (MQ=255) acacCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCgggg > 1:3305491/1‑69 (MQ=255) acacCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCggg > 1:1189641/1‑68 (MQ=255) acacCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTT‑TACGCCgggg > 1:2625317/1‑68 (MQ=255) acacCGCCGTTCACCAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCgggg < 1:2815653/69‑1 (MQ=255) acCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGCa < 1:1649416/69‑1 (MQ=255) | GGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGCA > minE/188833‑188958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |