| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,160,326 | G→A | 100% | intergenic (+46/+2) | sfsB → / ← murA | DNA‑binding transcriptional regulator/UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,160,326 | 0 | G | A | 75.8% | 41.9 / 9.3 | 37 | intergenic (+46/+2) | sfsB/murA | DNA‑binding transcriptional regulator/UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (8/1); new base A (22/6); total (30/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTT > minE/2160261‑2160389 | acacTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGatt > 1:2615083/1‑69 (MQ=255) cTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAatat > 1:3398431/1‑68 (MQ=255) aCGGGGCTGCCAGCTCTCAGACAATTTCTCGCCTTTCACACACTCAatat > 1:3060344/1‑50 (MQ=255) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCaa > 1:100548/1‑47 (MQ=37) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:3268324/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:3144591/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:333052/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:338108/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:2569262/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:2478800/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:2452422/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:2427428/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:2368090/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:2261598/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:2241199/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:1999964/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:1917651/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:1819534/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:417232/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:1540985/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:702525/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:706253/1‑50 (MQ=38) aCGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:1197254/1‑50 (MQ=38) aCGGGGCTGACAGCTCTCAGACAATTACTCGCCTTTCACACACTCAatat > 1:125292/1‑50 (MQ=37) cGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGt < 1:661908/69‑1 (MQ=255) cGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGt < 1:716199/69‑1 (MQ=255) cGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGt < 1:1177839/69‑1 (MQ=255) cGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGt < 1:1476594/69‑1 (MQ=255) cGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGt < 1:1393731/69‑1 (MQ=255) aGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTc < 1:1184451/67‑1 (MQ=255) aGCTCTCAGACAATTACTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGt < 1:1218481/59‑1 (MQ=38) aGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGtt > 1:313886/1‑68 (MQ=255) aGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGtt > 1:2843212/1‑68 (MQ=255) aGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGtt > 1:2528436/1‑68 (MQ=255) aGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGtt > 1:1798526/1‑68 (MQ=255) aGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGtt > 1:594983/1‑68 (MQ=255) aGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGtt > 1:904942/1‑68 (MQ=255) | ACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTT > minE/2160261‑2160389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |