| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,160,370 | C→T | 62.8% | L406L (CTG→CTA) | murA ← | UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,160,370 | 0 | C | T | 62.8% | 25.7 / 29.7 | 51 | L406L (CTG→CTA) | murA | UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (13/6); new base T (5/27); total (18/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.28e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCA > minE/2160305‑2160425 | cGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGt < 1:1177839/69‑1 (MQ=255)cGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGt < 1:716199/69‑1 (MQ=255)cGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGt < 1:661908/69‑1 (MQ=255)cGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGt < 1:1393731/69‑1 (MQ=255)cGGGGCTGCCAGCTCTCAGACAATTACTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGt < 1:1476594/69‑1 (MQ=255) aGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTc < 1:1184451/67‑1 (MQ=255) aGCTCTCAGACAATTACTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGt < 1:1218481/59‑1 (MQ=38) aGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGtt > 1:904942/1‑68 (MQ=255) aGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGtt > 1:594983/1‑68 (MQ=255) aGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGtt > 1:313886/1‑68 (MQ=255) aGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGtt > 1:2843212/1‑68 (MQ=255) aGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGtt > 1:2528436/1‑68 (MQ=255) aGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGtt > 1:1798526/1‑68 (MQ=255) cTTTCACACGCTGAATATTTGCACCTAAAGCGCGTTGTTTGTCTTCAATGCGTTCGTAg < 1:2366518/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:1740061/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:1129829/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:783019/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:756418/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:1228294/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:1266045/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:65796/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:621891/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:372937/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:3426477/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:3377743/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:3346560/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:1313/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:2905264/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:2792112/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:2116426/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:1726231/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:2170123/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:1603522/59‑1 (MQ=255) cTTTCACACGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAg < 1:1753110/59‑1 (MQ=255) aggCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATa > 1:1950984/3‑69 (MQ=255) acGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAGCCAcga > 1:586778/1‑58 (MQ=255) acGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAt > 1:325759/1‑68 (MQ=255) acGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATa > 1:3215025/1‑69 (MQ=255) acGCTCAATATTTGCACCTAAAGCGCGTAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATa > 1:445283/1‑69 (MQ=255) cGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAt > 1:3080418/1‑67 (MQ=255) cGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATa > 1:2310671/1‑68 (MQ=255) cGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATa > 1:1436672/1‑68 (MQ=255) cGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATa > 1:1365179/1‑68 (MQ=255) tatTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAg > 1:2329008/1‑44 (MQ=255) tttGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCAcgatc < 1:76345/50‑1 (MQ=255) tttGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCAcgatc < 1:2911120/50‑1 (MQ=255) tttGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCAcgatc < 1:1827222/50‑1 (MQ=255) tttGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCAcgatc < 1:1301629/50‑1 (MQ=255) tttGCACCTAAAGCGCCCAGTTTGTCTTCAATGCGTTCGTAGCCAcgatc < 1:528484/50‑1 (MQ=255) cACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAacca > 1:57647/1‑69 (MQ=255) cACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAacca > 1:2344505/1‑69 (MQ=255) | CGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCA > minE/2160305‑2160425 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |