| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,167,425 | A→G | 41.5% | I20M (ATA→ATG) | kdsC → | 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,167,425 | 0 | A | G | 41.5% | 57.5 / 115.5 | 154 | I20M (ATA→ATG) | kdsC | 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate phosphatase |
| Reads supporting (aligned to +/- strand): ref base A (56/34); new base G (17/47); total (73/81) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.71e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 157 total aligned reads displayed.
AAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATT > minE/2167373‑2167491 | gCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATGGCaaa > 1:599038/1‑56 (MQ=255) gCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACAtt > 1:2928251/1‑69 (MQ=255) gCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACAtt > 1:1871826/1‑69 (MQ=255) gCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACAtt > 1:3127104/1‑69 (MQ=255) gCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACAtt > 1:3280224/1‑69 (MQ=255) gCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACAtt > 1:1366786/1‑69 (MQ=255) gCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACAt > 1:2158387/1‑68 (MQ=255) tGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTct > 1:1953616/1‑69 (MQ=255) agCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTc > 1:2884846/2‑68 (MQ=255) tCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTctgctg < 1:2916514/69‑1 (MQ=255) tCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTctgctg < 1:2528840/69‑1 (MQ=255) tCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTctgctg < 1:1217246/69‑1 (MQ=255) tCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTctgctg < 1:195898/69‑1 (MQ=255) tCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTctgctg < 1:1343188/69‑1 (MQ=255) tCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTctgctg < 1:793042/69‑1 (MQ=255) cGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTctgctg < 1:736479/68‑1 (MQ=255) gACCTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCtc < 1:1016547/67‑1 (MQ=255) gACCTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCtc < 1:140387/67‑1 (MQ=255) gACCTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCtc < 1:784657/67‑1 (MQ=255) gACCTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCtc < 1:1769414/67‑1 (MQ=255) gACCTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCtc < 1:2374923/67‑1 (MQ=255) ccTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgacg > 1:3396006/1‑67 (MQ=255) ccTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgacg > 1:1524422/1‑67 (MQ=255) ccTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgacg > 1:428094/1‑67 (MQ=255) ccTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgacg > 1:2676092/1‑67 (MQ=255) ccTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgacg > 1:3110809/1‑67 (MQ=255) ccTGTTACGGCCCTGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgac > 1:2210435/1‑67 (MQ=255) tGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACAt < 1:2128801/47‑1 (MQ=255) tGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACAt < 1:3247052/47‑1 (MQ=255) tACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgatgtc > 1:2939707/1‑66 (MQ=255) tACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgatgtc > 1:1571509/1‑66 (MQ=255) tACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgatgtc > 1:2800415/1‑66 (MQ=255) tACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgatgtc > 1:1251504/1‑66 (MQ=255) tACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgatgtc > 1:1116779/1‑66 (MQ=255) tACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgatgtc > 1:647970/1‑66 (MQ=255) tACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgatgtc < 1:76498/66‑1 (MQ=255) aCGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGaa < 1:1488558/40‑1 (MQ=255) aCGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGaa > 1:147938/1‑40 (MQ=255) aCGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGaa < 1:2153380/40‑1 (MQ=255) aCGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGaa < 1:2355441/40‑1 (MQ=255) aCGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgatgtcgatg > 1:2386558/1‑69 (MQ=255) aCGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgatgtcgatg > 1:368404/1‑69 (MQ=255) aCGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgatgtcgatg > 1:2730125/1‑69 (MQ=255) aCGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgatgtcgatg > 1:501161/1‑69 (MQ=255) aCGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgatgtcgatg > 1:2667655/1‑69 (MQ=255) gCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtc > 1:3132430/1‑56 (MQ=255) gCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtc > 1:555618/1‑56 (MQ=255) gCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCg < 1:2447318/69‑1 (MQ=255) gCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCg < 1:793920/69‑1 (MQ=255) gCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCg < 1:821755/69‑1 (MQ=255) gCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCg < 1:618580/69‑1 (MQ=255) gCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCg < 1:1654542/69‑1 (MQ=255) gCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAAAATTCg > 1:355952/1‑43 (MQ=255) ccTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTa > 1:2167869/1‑69 (MQ=255) ccTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTa > 1:3103959/1‑69 (MQ=255) ccTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTa > 1:2152533/1‑69 (MQ=255) ccTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTa > 1:1329174/1‑69 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGATCATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:2246198/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:1935544/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:1475984/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:1535042/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:525341/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:1461651/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:1331640/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:1896991/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:1330570/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:2753919/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:1018813/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:1055563/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:2205526/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:2589430/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:2607880/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:2639522/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:2644346/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:2139622/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:3003329/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:2795347/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:684561/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:2822162/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:2024797/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:1083643/69‑1 (MQ=255) tGTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:1900964/69‑1 (MQ=255) gTCAGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGACGTTGATGGCGTACt < 1:1834281/68‑1 (MQ=255) tCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgat > 1:38498/1‑53 (MQ=255) tCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgat > 1:1208417/1‑53 (MQ=255) tCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgat > 1:3353889/1‑53 (MQ=255) tCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgat > 1:1191162/1‑53 (MQ=255) tCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgat > 1:322100/1‑53 (MQ=255) tCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgat > 1:1943321/1‑53 (MQ=255) tCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgat > 1:2113467/1‑53 (MQ=255) aGCGCCGTCGTTATGGCATAAGCAGTGAACATTCGTCTGCTGATCCtc < 1:823360/48‑1 (MQ=255) aGCGCCGACGTTATGGCAAAAGCAGAGAACATTCGTCTGCTGATCCtc < 1:3312220/48‑1 (MQ=255) aGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCa < 1:939754/69‑1 (MQ=255) aGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCa < 1:68768/69‑1 (MQ=255) aGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCa < 1:2086687/69‑1 (MQ=255) ccGACGTTATAGCATAAGCAGAGAACATTCGTctgctg < 1:334795/38‑1 (MQ=255) ttATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGa > 1:470470/1‑69 (MQ=255) ataGCAAAAGCAGAGAACATTCGTCTGCTGATTCTCGATGTCGATGGCGTACTGTCAGATGGCCTGAtt > 1:2533554/1‑69 (MQ=255) ataGCAAAAGCAGAGAACATTCGTCTGCTGATCCtcgatgtcgatg > 1:2486924/1‑46 (MQ=255) ataGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGAtt > 1:3177344/1‑69 (MQ=255) | AAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATT > minE/2167373‑2167491 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |