| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,171,234 | A→G | 45.1% | Q475Q (CAA→CAG) | rpoN → | RNA polymerase, sigma 54 (sigma N) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,171,234 | 0 | A | G | 45.1% | 15.0 / 84.8 | 102 | Q475Q (CAA→CAG) | rpoN | RNA polymerase, sigma 54 (sigma N) factor |
| Reads supporting (aligned to +/- strand): ref base A (42/14); new base G (28/18); total (70/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
AAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACATTACCGGAAATAACGTCGAG > minE/2171187‑2171298 | aaGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATa > 1:663722/1‑68 (MQ=255)aaGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATa > 1:2762658/1‑68 (MQ=255)aaGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATa > 1:706692/1‑68 (MQ=255)aaGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACACAACCGATa > 1:1203008/1‑68 (MQ=255) aCCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGaa > 1:1173722/1‑69 (MQ=255) aCCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGaa > 1:2758729/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:2544202/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:185525/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:583841/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:1972636/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:2012456/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:2176490/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:3243560/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:230969/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:2539168/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:1163976/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:3068545/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:2752471/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:3047056/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:2894957/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:3022669/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:1738322/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:636017/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:150269/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:11966/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:1738138/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:1306114/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:1681894/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGac > 1:845128/1‑69 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGa < 1:257394/68‑1 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGa < 1:1511511/68‑1 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGa < 1:1574019/68‑1 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGa < 1:2196046/68‑1 (MQ=255) gagaGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGa < 1:1741018/68‑1 (MQ=255) agagTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGa < 1:1504767/67‑1 (MQ=255) gagTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGacac < 1:3249647/69‑1 (MQ=255) gagTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGacac < 1:3390683/69‑1 (MQ=255) gagTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGacac < 1:418299/69‑1 (MQ=255) gagTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGacac < 1:2858620/69‑1 (MQ=255) agTCTTTATCCATTCCGCCGTCAAACCAGTGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACt < 1:2265950/69‑1 (MQ=255) agTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACt < 1:2320580/69‑1 (MQ=255) cATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:2762960/69‑1 (MQ=255) cATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:3053294/69‑1 (MQ=255) cATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:1170153/69‑1 (MQ=255) cATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:1105869/69‑1 (MQ=255) cATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:260877/69‑1 (MQ=255) cATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:3184575/69‑1 (MQ=255) cATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:2590224/69‑1 (MQ=255) cATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:772757/69‑1 (MQ=255) cATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:3189018/69‑1 (MQ=255) cATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:696230/69‑1 (MQ=255) cATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:3279708/69‑1 (MQ=255) cATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:386323/69‑1 (MQ=255) cATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:1694463/69‑1 (MQ=255) cATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:191057/69‑1 (MQ=255) cATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:516946/69‑1 (MQ=255) ttCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:2969353/67‑1 (MQ=255) tCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAAc > 1:3135648/1‑68 (MQ=255) ccgccgTCAAACCAGCGTAAACAGCTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa < 1:3101636/65‑1 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGa > 1:489996/1‑51 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGa > 1:2719219/1‑51 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGa > 1:2595414/1‑51 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGa > 1:231823/1‑51 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGa > 1:2109434/1‑51 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGc > 1:731032/1‑59 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCa > 1:40074/1‑60 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCa > 1:869935/1‑60 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCa > 1:1875648/1‑60 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCa > 1:1911931/1‑60 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCa > 1:3433829/1‑60 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCa > 1:34154/1‑60 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACAt > 1:722516/1‑69 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACAt > 1:2378715/1‑69 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACAt > 1:517195/1‑69 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACAt > 1:428377/1‑69 (MQ=255) ccgccgTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACAt > 1:1899507/1‑69 (MQ=255) aCCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACATTACCGGAAAt < 1:620959/69‑1 (MQ=255) tAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCaa > 1:2756654/1‑49 (MQ=255) tAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCaa > 1:2132500/1‑49 (MQ=255) tAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCaa > 1:1312519/1‑49 (MQ=255) tAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCaa > 1:803697/1‑49 (MQ=255) tAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCaa > 1:1639599/1‑49 (MQ=255) tAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCaa > 1:672941/1‑49 (MQ=255) tAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCaa > 1:1792316/1‑49 (MQ=255) tAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCaa > 1:1947412/1‑49 (MQ=255) tAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCaa > 1:2789853/1‑49 (MQ=255) tAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCaa > 1:2076866/1‑49 (MQ=255) tAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCaa > 1:2370056/1‑49 (MQ=255) tAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCa > 1:2129978/1‑48 (MQ=255) aacaacTCGTTTGACCCAACCGATAAGGAAGAGACTATGCAGCTCAACATTACCGGa > 1:1781672/1‑57 (MQ=255) aacaacTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAAc > 1:1551357/1‑48 (MQ=255) aacaacTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACa > 1:2237792/1‑49 (MQ=255) aacaacTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACATTACCgg > 1:3403818/1‑56 (MQ=255) aacaacTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACATTACCGGa > 1:567528/1‑57 (MQ=255) aacaacTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACATTACCGGa > 1:2538883/1‑57 (MQ=255) aacaacTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACATTACCGGa > 1:1539287/1‑57 (MQ=255) aacaacTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACATTACCGGa > 1:3268219/1‑57 (MQ=255) aacaacTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACATTACCGGAAATAACg > 1:3434925/1‑64 (MQ=255) aacaacTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACATTACCGGAAATAACGTCgag < 1:3132006/69‑1 (MQ=255) aacaacTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACATTACCGGAAATAACGTCgag < 1:3125385/69‑1 (MQ=255) | AAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACCCAACCGATAAGGAAGACACTATGCAGCTCAACATTACCGGAAATAACGTCGAG > minE/2171187‑2171298 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |