| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,172,453 | C→A | 56.6% | L82L (CTC→CTA) | yhbJ → | hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,172,453 | 0 | C | A | 56.6% | 20.8 / 144.4 | 129 | L82L (CTC→CTA) | yhbJ | hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base C (19/37); new base A (28/45); total (47/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01 | |||||||||||
Only 100 of 131 total aligned reads displayed.
TGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCA > minE/2172386‑2172515 | tGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTcc > 1:3391521/1‑69 (MQ=255) tCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTg < 1:1032879/69‑1 (MQ=255) tCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTg < 1:950259/69‑1 (MQ=255) tCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTg < 1:930540/69‑1 (MQ=255) tCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTg < 1:2946107/69‑1 (MQ=255) tCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTg < 1:2938666/69‑1 (MQ=255) tCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTg < 1:2890947/69‑1 (MQ=255) tCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTg < 1:2672732/69‑1 (MQ=255) aCCAGAAATATTCGAACAGGCGATGAGTACCCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGa > 1:1200709/1‑69 (MQ=255) aCCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCTACTCCTGTTCCTGGa > 1:503345/1‑69 (MQ=255) aCCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTg > 1:2254918/1‑67 (MQ=255) aCCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGa > 1:316028/1‑69 (MQ=255) aCCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGa > 1:167312/1‑69 (MQ=255) aCCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGa > 1:2036322/1‑69 (MQ=255) aCCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGa > 1:3362294/1‑69 (MQ=255) aCCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGa > 1:2550860/1‑69 (MQ=255) aCCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGa > 1:2730975/1‑69 (MQ=255) aCCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGa > 1:1288611/1‑69 (MQ=255) aCCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGa > 1:3291044/1‑69 (MQ=255) ccAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTg < 1:653442/66‑1 (MQ=255) ttCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGt < 1:2769795/69‑1 (MQ=255) ttCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGt < 1:2425724/69‑1 (MQ=255) ttCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGt < 1:3327958/69‑1 (MQ=255) ttCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGt < 1:3021811/69‑1 (MQ=255) ttCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGt < 1:1140551/69‑1 (MQ=255) ttCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGt < 1:584787/69‑1 (MQ=255) ttCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGt < 1:1224063/69‑1 (MQ=255) ttCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGt < 1:820777/69‑1 (MQ=255) cGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGTaa < 1:2252823/69‑1 (MQ=255) cGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGTaa < 1:1440626/69‑1 (MQ=255) cGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGTaa < 1:1252118/69‑1 (MQ=255) cGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGTaa < 1:2916099/69‑1 (MQ=255) gAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGTaa < 1:678214/68‑1 (MQ=255) gCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGTAATACCTTa < 1:2450935/69‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTCATACCt < 1:436355/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:2675920/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:1107651/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:2989759/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:3348376/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:826671/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:1070123/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:1309547/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:2824986/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:2521780/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:3215476/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:295008/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:1594336/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:2243956/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:2090794/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:2074056/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:1637444/65‑1 (MQ=255) gATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:3287623/65‑1 (MQ=255) tGAGTAACCTGCCTGACGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCt < 1:933427/63‑1 (MQ=255) tAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGTAATACCTTAATtcgtc > 1:3242469/1‑68 (MQ=255) cctgcctgACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGt < 1:2660238/49‑1 (MQ=255) cctgcctgACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGTaa < 1:3037956/51‑1 (MQ=255) tgACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGa < 1:2274100/69‑1 (MQ=255) aCGTTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:2527101/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:3172903/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:669670/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:1189093/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:3273865/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:711517/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:1038673/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:352602/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:509962/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:572165/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:3031269/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:1277776/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:1510406/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:1742080/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:175107/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:1874416/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:1887305/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:1932190/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:20098/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTaa > 1:1070298/1‑51 (MQ=255) aCGCTTTCTCACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTa > 1:2282108/1‑50 (MQ=255) aCGCTTTCTCACCGCAACTAATGTTCCTGGATGCCGACCGTAATACCTTaa > 1:371506/1‑51 (MQ=255) cGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCAACCGTAATACCTTAATTCGTCGTTACAGTGacac > 1:2526688/1‑69 (MQ=255) ctcAGCGCAACTACTGTTCCTGGATGCCGAGCGTAATACCTTAATtc < 1:2699240/47‑1 (MQ=255) ctcACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTAATtc < 1:2314895/47‑1 (MQ=255) ctcACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTAATtc < 1:102904/47‑1 (MQ=255) ctcACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTAATtc < 1:3146972/47‑1 (MQ=255) ctcACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTAATtc < 1:1036795/47‑1 (MQ=255) ctcACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTAATtc < 1:1368826/47‑1 (MQ=255) ctcACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTAATtc < 1:3219345/47‑1 (MQ=255) ctcACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTAATtc < 1:70602/47‑1 (MQ=255) ctcACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTAATtc < 1:2989629/47‑1 (MQ=255) ctcACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTAATtc < 1:2974375/47‑1 (MQ=255) ctcACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTAATtc < 1:643128/47‑1 (MQ=255) ctcACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTAATtc < 1:2953955/47‑1 (MQ=255) ctcACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTAATtc < 1:1051709/47‑1 (MQ=255) ctcACCGCAACTACTGTTCCTGGATGCCGACCGTAATACCTTAATtc < 1:2301200/47‑1 (MQ=255) tcACCGCAACTCCTGTTCCTGGATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGcc < 1:732997/67‑1 (MQ=255) tcACCGCAACTCCTGTTCCTGGATGACGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGcc < 1:1331218/67‑1 (MQ=255) gCAACTCCTGTTCCTGGATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGc > 1:873062/1‑68 (MQ=255) gCAACTCCTGTTCCTGGATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGc > 1:393768/1‑68 (MQ=255) gCAACTCCTGTTCCTGGATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCa > 1:562225/1‑69 (MQ=255) aaCTACTGTTCCTGGATGCCGACCGTAATACCTTAATtc < 1:1787723/39‑1 (MQ=255) | TGCCGGAGTCACCAGAAATATTCGAACAGGCGATGAGTAACCTGCCTGACGCTTTCTCACCGCAACTCCTGTTCCTGGATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCA > minE/2172386‑2172515 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |