| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,173,038 | T→G | 25.8% | R277R (CGT→CGG) | yhbJ → | hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,173,038 | 0 | T | G | 25.8% | 172.5 / 71.4 | 128 | R277R (CGT→CGG) | yhbJ | hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base T (58/37); new base G (20/13); total (78/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 128 total aligned reads displayed.
TATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCA > minE/2172975‑2173105 | tatTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGc > 1:1866925/1‑68 (MQ=255) ttGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTg > 1:2310288/1‑68 (MQ=255) gagCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGaaaaa < 1:907637/69‑1 (MQ=255) gagCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGaaaaa < 1:2762255/69‑1 (MQ=255) gagCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGaaaaa < 1:2361983/69‑1 (MQ=255) gagCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGaaaaa < 1:3218981/69‑1 (MQ=255) gagCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGaaaaa < 1:715421/69‑1 (MQ=255) gagCAACTGGCAGACTACTTCCGCTCGCGCGGCAAAAACGTCCAGTCACGCCATCGTACGCTGGaaaaa < 1:3026660/69‑1 (MQ=255) agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGaaaaa < 1:516914/68‑1 (MQ=255) tGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAAc < 1:1956620/69‑1 (MQ=255) tGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAAc < 1:603423/69‑1 (MQ=255) ctgCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAAc < 1:767313/67‑1 (MQ=255) ctactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCAt > 1:2874542/1‑65 (MQ=255) ctactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCAt > 1:3224531/1‑65 (MQ=255) ctactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCAt > 1:343607/1‑65 (MQ=255) ctactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCAt > 1:3220605/1‑65 (MQ=255) ctactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCAt > 1:2302880/1‑65 (MQ=255) ctactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCAt > 1:536801/1‑65 (MQ=255) ctactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCAt > 1:1364250/1‑65 (MQ=255) tactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAAc > 1:511478/1‑55 (MQ=255) tactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGAcc > 1:1145634/1‑68 (MQ=255) tactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGAcc > 1:21466/1‑68 (MQ=255) tactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGAcc > 1:3023013/1‑68 (MQ=255) tactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGAcc > 1:2807472/1‑68 (MQ=255) tactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGAcc > 1:2334643/1‑68 (MQ=255) tactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGAcc > 1:780174/1‑68 (MQ=255) tactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGAcc > 1:1818846/1‑68 (MQ=255) tactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGAcc > 1:177211/1‑68 (MQ=255) tactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGAcc > 1:417222/1‑68 (MQ=255) ctTCCGCTCGCGCGGTAAAAACGTCCAGTCAGGCCATCGTACGCTGGAAAAACGTaaa > 1:3330980/1‑58 (MQ=255) ctTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTaaa > 1:2648483/1‑58 (MQ=255) ctTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAAc < 1:1902396/59‑1 (MQ=255) ctTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAAc < 1:2975692/59‑1 (MQ=255) ctTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAAc < 1:2383308/59‑1 (MQ=255) ctTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAAc < 1:557012/59‑1 (MQ=255) ctTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAAc < 1:1305518/59‑1 (MQ=255) ctTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAAc > 1:1567613/1‑59 (MQ=255) ctTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAAc > 1:26472/1‑59 (MQ=255) ctTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAAc > 1:2210950/1‑59 (MQ=255) ctTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAAc < 1:2161824/59‑1 (MQ=255) ctTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAAc > 1:3345838/1‑59 (MQ=255) ctTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAAc > 1:1769874/1‑59 (MQ=255) ttCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAAc < 1:2990106/58‑1 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCa < 1:1083825/68‑1 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCa < 1:152415/68‑1 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCa < 1:1369090/68‑1 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCa < 1:1602690/68‑1 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCa < 1:2566171/68‑1 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGt > 1:2972803/1‑49 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGTAAACCATGACCg > 1:673266/1‑61 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGTAAACCATGACCg > 1:1020242/1‑61 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGTAAACCATGACCg > 1:3030002/1‑61 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGTAAACCATGACCg > 1:3242759/1‑61 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGTAAACCATGACCg > 1:3278848/1‑61 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGTAAACCATGACCg > 1:545766/1‑61 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGTAAACCATGACCg > 1:1009079/1‑61 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGTAAACCATGACCg > 1:2652960/1‑61 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGTAAACCATGACCg > 1:1032960/1‑61 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGTAAACCATGACCg > 1:2505261/1‑61 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGTAAACCATGACCg > 1:243266/1‑61 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGTAAACCATGACCg > 1:1725712/1‑61 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGTAAACCATGACCg > 1:224828/1‑61 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGTAAACCATGACCg > 1:2047260/1‑61 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGTAAACCATGACCg > 1:2159609/1‑61 (MQ=255) cTCGCGCGGTAAAAACGTCCAGTCACGCCATCGGACGCTGGAAAAACGTAAAACATGACCg > 1:2865086/1‑61 (MQ=255) cgcgcgGTAAAAACGTTCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAAc > 1:1032015/1‑69 (MQ=255) cgcgcgGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAAc > 1:2371270/1‑69 (MQ=255) tCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaa > 1:1904059/1‑68 (MQ=255) tCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaa > 1:3304693/1‑68 (MQ=255) tCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaa > 1:3243589/1‑68 (MQ=255) tCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaa > 1:3127251/1‑68 (MQ=255) tCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaa > 1:2232523/1‑68 (MQ=255) tCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaa > 1:1136029/1‑68 (MQ=255) gCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTggg < 1:139701/69‑1 (MQ=255) gCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTggg < 1:156636/69‑1 (MQ=255) gCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTggg < 1:486895/69‑1 (MQ=255) gCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTggg < 1:62244/69‑1 (MQ=255) gCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTggg < 1:334640/69‑1 (MQ=255) gCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTggg < 1:3319979/69‑1 (MQ=255) gCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTggg < 1:2759354/69‑1 (MQ=255) gCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTggg < 1:2767903/69‑1 (MQ=255) gCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTggg < 1:2984128/69‑1 (MQ=255) gCCATCGGACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTgg < 1:1673122/68‑1 (MQ=255) gCCATCGGACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTgg < 1:166910/68‑1 (MQ=255) gCCATCGGACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTgg < 1:1134862/68‑1 (MQ=255) gCCATCGGACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTgg < 1:1617334/68‑1 (MQ=255) gCCATCGGACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTgg < 1:1220875/68‑1 (MQ=255) aTCGGACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTgg < 1:516989/65‑1 (MQ=255) gTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatgca > 1:2670792/1‑69 (MQ=255) gTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatgca > 1:2623363/1‑69 (MQ=255) gTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatgca > 1:1237553/1‑69 (MQ=255) gTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatgca > 1:1294562/1‑69 (MQ=255) gTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatgca > 1:2190889/1‑69 (MQ=255) gTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatgca > 1:2068844/1‑69 (MQ=255) gTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatgca > 1:2001994/1‑69 (MQ=255) gTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatgca > 1:46370/1‑69 (MQ=255) gTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatgca > 1:3350682/1‑69 (MQ=255) gTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatgca > 1:335014/1‑69 (MQ=255) gTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatgca > 1:3309530/1‑69 (MQ=255) gTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCAAAAACAAGCTGGgcatgca > 1:2304512/1‑69 (MQ=255) | TATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCA > minE/2172975‑2173105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |