| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 189,526 | G→A | 27.6% | P341P (CCG→CCA) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 189,526 | 0 | G | A | 27.6% | 78.5 / 27.7 | 72 | P341P (CCG→CCA) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (32/20); new base A (15/5); total (47/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAA > minE/189458‑189587 | aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCg < 1:1072482/69‑1 (MQ=255)aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCg < 1:3162503/69‑1 (MQ=255)aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCg < 1:3294136/69‑1 (MQ=255)aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCg < 1:2969748/69‑1 (MQ=255)aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCg < 1:3351253/69‑1 (MQ=255)aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCg < 1:2816577/69‑1 (MQ=255)aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCg < 1:346278/69‑1 (MQ=255)aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCg < 1:2553922/69‑1 (MQ=255)aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCg < 1:568000/69‑1 (MQ=255)aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCg < 1:171559/69‑1 (MQ=255)aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCg < 1:672794/69‑1 (MQ=255)aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCg < 1:86017/69‑1 (MQ=255) aaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCg < 1:3386178/68‑1 (MQ=255) cAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTTCCTGGGTGCCGTACACCCATTTTCATCCGATc < 1:662052/69‑1 (MQ=255) cAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTGCACCCATTTTCATCCGATc < 1:1529329/69‑1 (MQ=255) cAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATc < 1:2577477/69‑1 (MQ=255) cAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATc < 1:724148/69‑1 (MQ=255) cAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATc < 1:2315409/69‑1 (MQ=255) cAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATc < 1:586565/69‑1 (MQ=255) cAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATc < 1:2308594/69‑1 (MQ=255) cAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATc < 1:2119418/69‑1 (MQ=255) cAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATc < 1:1854215/69‑1 (MQ=255) cAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATc < 1:926613/69‑1 (MQ=255) cAGACGCTGGATGTCCCGTCGATTCACTTAGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATc < 1:2766708/69‑1 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGt > 1:760996/1‑69 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGt > 1:2009955/1‑69 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGt > 1:2005103/1‑69 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGt > 1:1465676/1‑69 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGt > 1:35297/1‑69 (MQ=255) tCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAgcggcg > 1:50381/1‑69 (MQ=255) tCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAgcggcg > 1:579033/1‑69 (MQ=255) tCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAgcggcg > 1:2327839/1‑69 (MQ=255) tCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAgcggcg > 1:1843880/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCgg > 1:2208962/1‑59 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:377498/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:364129/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:714235/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:54795/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:624837/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:3321566/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:869181/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:3263124/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:3241961/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:3065936/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:1332358/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:1394297/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:2939980/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:2833938/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:2464134/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:2468456/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:2631389/1‑69 (MQ=255) cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:2573134/1‑69 (MQ=255) cacCCATTTTCATCCGATCTAACAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:1086996/1‑69 (MQ=255) cacCCATTTTCATCCGATATACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:834374/1‑69 (MQ=255) catttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCg < 1:2063078/67‑1 (MQ=255) aTTTTCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCg < 1:3198025/69‑1 (MQ=255) aTTTTCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCg < 1:1658421/69‑1 (MQ=255) aTTTTCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCg < 1:2494850/69‑1 (MQ=255) aTTTTCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCg < 1:2784503/69‑1 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:2996070/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:776662/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:1004670/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:72894/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:868209/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:3147853/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:882276/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:639226/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:62321/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:1081765/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:336837/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:940438/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:253841/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:993747/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:260693/1‑69 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTg < 1:417747/59‑1 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTg < 1:83950/59‑1 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTg < 1:1387401/59‑1 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTg < 1:1581250/59‑1 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTg < 1:3281932/59‑1 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTg < 1:2678313/59‑1 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTg < 1:422737/59‑1 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGaaa > 1:3016047/1‑69 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGaaa > 1:23135/1‑69 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTCCGGGAAAAGTg < 1:118681/59‑1 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGAGAGCGTGTTGCGGGAAAAGTg < 1:3278756/59‑1 (MQ=255) | AAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAA > minE/189458‑189587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |