| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,177,633 | A→G | 42.4% | D162D (GAT→GAC) | arcB ← | hybrid sensory histidine kinase in two‑component regulatory system with ArcA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,177,633 | 0 | A | G | 42.4% | 16.6 / 49.2 | 66 | D162D (GAT→GAC) | arcB | hybrid sensory histidine kinase in two‑component regulatory system with ArcA |
| Reads supporting (aligned to +/- strand): ref base A (18/20); new base G (11/17); total (29/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTTACAGCCGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTTGTGTCTCT > minE/2177575‑2177687 | ggTTACAGCCGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCaaggaagg < 1:730423/69‑1 (MQ=255)ggTTACAGCCGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCaaggaagg < 1:65360/69‑1 (MQ=255)ggTTACAGCCGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCaaggaagg < 1:827035/69‑1 (MQ=255)ggTTACAGCCGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCaaggaagg < 1:2978874/69‑1 (MQ=255)ggTTACAGCCGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCaaggaagg < 1:2832909/69‑1 (MQ=255)ggTTACAGCCGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCaaggaagg < 1:2258311/69‑1 (MQ=255)ggTTACAGCCGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCaaggaagg < 1:1823646/69‑1 (MQ=255)ggTTACAGCCGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCaaggaagg < 1:837850/69‑1 (MQ=255) gTTACAGCCGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCaaggaagg < 1:2489530/68‑1 (MQ=255) aCAGCCGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAggaaggaa > 1:619428/1‑67 (MQ=255) aCAGCCGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAAc > 1:196527/1‑68 (MQ=255) aCAGCCGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTAGGGTGAAGCATCAAGGAAGGAAc > 1:2517176/1‑68 (MQ=255) aCAACCGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAggaaggaa > 1:3263247/1‑67 (MQ=255) cGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGa < 1:861806/69‑1 (MQ=255) cGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGa < 1:2562171/69‑1 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCa > 1:1926377/1‑43 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGaa > 1:3079641/1‑63 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg < 1:2455472/67‑1 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg > 1:2421247/1‑67 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg < 1:744917/67‑1 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg > 1:364704/1‑67 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg > 1:1101742/1‑67 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg > 1:2779768/1‑67 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg < 1:2802299/67‑1 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg > 1:2804375/1‑67 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg > 1:3038456/1‑67 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg > 1:3208582/1‑67 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg < 1:241127/67‑1 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg < 1:1787703/67‑1 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg > 1:1692263/1‑67 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg > 1:1924469/1‑67 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg > 1:1971489/1‑67 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg > 1:2064573/1‑67 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg > 1:1604980/1‑67 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg > 1:564110/1‑67 (MQ=255) ctctTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAg < 1:2371504/67‑1 (MQ=255) aCGATAAAAAACCAGGTCGGGTGAAGCATCaaggaagg < 1:2134847/38‑1 (MQ=255) aCGATAAAAAACCAGGTCGGGTGAAGCATCaaggaagg < 1:613991/38‑1 (MQ=255) aCGATAAAAAACCAGGTCGGGTGAAGCATCaaggaagg < 1:1531206/38‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:3307725/68‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:652663/68‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:3257149/68‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:1248099/68‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:738704/68‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:957731/68‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:3175429/68‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:828018/68‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:2804072/68‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:2734492/68‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:2559586/68‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:2338574/68‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:2263033/68‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:221281/68‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:2099648/68‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:1971056/68‑1 (MQ=255) aaaCCAGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTtgt < 1:1657570/68‑1 (MQ=255) aGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTg > 1:847118/1‑56 (MQ=255) aGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAAt > 1:2267433/1‑59 (MQ=255) aGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTTGTGTctct > 1:2687690/1‑69 (MQ=255) aGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTTGTGTctct > 1:53458/1‑69 (MQ=255) aGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTTGTGTctct > 1:385249/1‑69 (MQ=255) aGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTTGTGTctct > 1:3049562/1‑69 (MQ=255) aGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTTGTGTctct > 1:304684/1‑69 (MQ=255) aGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTTGTGTctct > 1:1672298/1‑69 (MQ=255) aGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTTGTGTctct > 1:1583861/1‑69 (MQ=255) aGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTTGTGTctct > 1:1581642/1‑69 (MQ=255) aGGTCGGGTGAAGCGTCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTTGTGTctct > 1:1215270/1‑69 (MQ=255) | GGTTACAGCCGGAAAACTCTTTATCTTCGTTACGATAAAAAACCAGGTCGGGTGAAGCATCAAGGAAGGAACGTAAGAATGAGGATTGCTGCTCGAGCTGAATTTGTGTCTCT > minE/2177575‑2177687 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |