| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,182,550 | T→G | 56.3% | A942A (GCT→GCG) | gltB → | glutamate synthase, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,182,550 | 0 | T | G | 56.3% | 13.1 / 26.2 | 32 | A942A (GCT→GCG) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base T (4/10); new base G (16/2); total (20/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.90e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGAAGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAA > minE/2182486‑2182609 | gcgAAGGCGGTGAAGACCCGGCGCGCTATGGCACTAACAAAGTGTCGCGCATCAAGCAGGTGGCGTc < 1:1109174/65‑1 (MQ=255) aaGGCGGTGAAGACCCGGCGCGCTATGGCACTAACACAGTGTCGCGCATCAAGCAGGTGGCGTc < 1:1653985/64‑1 (MQ=255) gAAGACCCGGCGCGCTATGGCACTAACAAAGTGTCGCGCATGAAGCAGGTGGCGTCCGGTCGCTTCggc > 1:1555900/1‑68 (MQ=255) gAAGACCCGGCGCGCTATGGCACTAACAAAGTGTCGCGCATCAAGCAGGTGGCGTCCGGTCGCTTCggc > 1:962314/1‑68 (MQ=255) gAAGACCCGGCGCGCTATGGCACTAACAAAGTGTCGCGCATCAAGCAGGTGGCGTCCGGTCGCTTCggc > 1:79530/1‑68 (MQ=255) gAAGACCCGGCGCGCTATGGCACTAACAAAGTGTCGCGCATCAAGCAGGTGGCGTCCGGTCGCTTCggc > 1:672859/1‑68 (MQ=255) gAAGACCCGGCGCGCTATGGCACTAACAAAGTGTCGCGCATCAAGCAGGTGGCGTCCGGTCGCTTCggc > 1:544200/1‑68 (MQ=255) gAAGACCCGGCGCGCTATGGCACTAACAAAGTGTCGCGCATCAAGCAGGTGGCGTCCGGTCGCTTCggc > 1:423835/1‑68 (MQ=255) gAAGACCCGGCGCGCTATGGCACTAACAAAGTGTCGCGCATCAAGCAGGTGGCGTCCGGTCGCTTCggc > 1:400753/1‑68 (MQ=255) gAAGACCCGGCGCGCTATGGCACTAACAAAGTGTCGCGCATCAAGCAGGTGGCGTCCGGTCGCTTCggc > 1:3409152/1‑68 (MQ=255) gAAGACCCGGCGCGCTATGGCACTAACAAAGTGTCGCGCATCAAGCAGGTGGCGTCCGGTCGCTTCggc > 1:3127595/1‑68 (MQ=255) gAAGACCCGGCGCGCTATGGCACTAACAAAGTGTCGCGCATCAAGCAGGTGGCGTCCGGTCGCTTCggc > 1:1128306/1‑68 (MQ=255) gAAGACCCGGCGCGCTATGGCACTAACAAAGTGTCGCGCATCAAGCAGGTGGCGTCCGGTCGCTTCggc > 1:1260217/1‑68 (MQ=255) gAAGACCCGGCGCGCTATGGCACTAACAAAGTGTCGCGCATCAAGCAGGTGGCGTCCGGTCGCTTCggc > 1:1568388/1‑68 (MQ=255) gAAGACCCGGCGCGCTATGGCACTAACAAAGTGTCGCGCATCAAGCAGGTGGCGTCCGGTCGCTTCggc > 1:1618116/1‑68 (MQ=255) gAAGACCCAGCGCGCTATGGCACTAACAAAGTGTCGCGCATCAAGCAGGTGGCGTCCGGTCGCTTCggc > 1:3171711/1‑68 (MQ=255) gAAGACCAGGCGCGCTATGGCACTAACAAAGTGTCGCGCATCAAGCAGGTGGCGTCCGGTCGCTTCggc > 1:2184351/1‑68 (MQ=255) gAAGACACGGCGCGCTATGGCACTAACAAAGTGTCGCGCATCAAGCAGGTGGCGTCCGGTCGCTTCggc > 1:6142/1‑68 (MQ=255) cccGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTAc < 1:1300974/69‑1 (MQ=255) ccGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTAc < 1:1671492/68‑1 (MQ=255) gcTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGt < 1:1055417/69‑1 (MQ=255) aCCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTc < 1:3207788/69‑1 (MQ=255) gcgcATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTa > 1:3420742/1‑48 (MQ=255) gcgcATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTa > 1:2604035/1‑48 (MQ=255) cAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTaa < 1:803340/68‑1 (MQ=255) ggCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCa > 1:3221418/1‑57 (MQ=255) ggCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCa < 1:1370530/57‑1 (MQ=255) ggCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCa > 1:24952/1‑57 (MQ=255) ggCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCa < 1:630221/57‑1 (MQ=255) ggCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCa < 1:2062014/57‑1 (MQ=255) ggCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCa < 1:1898538/57‑1 (MQ=255) ggCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCAGACGTCATTCa < 1:2464472/57‑1 (MQ=255) | GTGAAGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAA > minE/2182486‑2182609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |