| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,183,135 | T→A | 30.9% | R1137R (CGT→CGA) | gltB → | glutamate synthase, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,183,135 | 0 | T | A | 30.9% | 77.2 / 44.9 | 68 | R1137R (CGT→CGA) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base T (24/23); new base A (9/12); total (33/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
CGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAGGATGACAAACTGCGTAAG > minE/2183075‑2183195 | cgcAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGGGGCGCTCGGCTGTAAATATCTACGTATTTGcc < 1:3257194/68‑1 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTAttt > 1:290469/1‑58 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGaac < 1:1024019/69‑1 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGaac > 1:2143589/1‑69 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGaac > 1:2189568/1‑69 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGaac > 1:1543498/1‑69 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGaac > 1:1407097/1‑69 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGaac > 1:526153/1‑69 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGaac > 1:816671/1‑69 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGaac > 1:1242417/1‑69 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGaac > 1:1115124/1‑69 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCAATGGTCGCGCTCGGTTGTAAATATCTGCGAATTTGCCACCTGaac > 1:3378898/1‑69 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCAATGGTCGCGCTCGGTTGTAAATATCTGCGAATTTGCCACCTGaac > 1:2333293/1‑69 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCAATGGTCGCGCTCGGTTGTAAATATCTGCGAATTTGCCACCTGaac > 1:2164066/1‑69 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCAATGGTCGCGCTCGGTTGTAAATATCTGCGAATTTGCCACCTGaac > 1:2220562/1‑69 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCAATGGTCGCGCTCGGTTGTAAATATCTGCGAATTTGCCACCTGaac > 1:2022886/1‑69 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCAATGGTCGCGCTCGGTTGTAAATATCTGCGAATTTGCCACCTGaac > 1:535709/1‑69 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCAATGGTCGCGCTCGGTTGTAAATATCTGCGAATTTGCCACCTGaac > 1:1632818/1‑69 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCAATGGTCGCGCTCGGTTGTAAATATCTGCGAATTTGCCACCTGaac > 1:1516898/1‑69 (MQ=255) aGCTTCGGCTTCGGCACTGGCCCAATGGTCGCGCTCGGTTGTAAATATCTGCGAATTTGCCACCTGaac > 1:2633564/1‑69 (MQ=255) cACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACggg < 1:193057/69‑1 (MQ=255) aCTGGCCCGATGGTGGCGCTCGGCTGTAAATACCTACGTATTTGCCATCTGAACAACTGCGCAACggg < 1:2075011/68‑1 (MQ=255) ggtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGaac > 1:3173238/1‑43 (MQ=255) ggtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGaac > 1:2203865/1‑43 (MQ=255) ggtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGaac > 1:1634909/1‑43 (MQ=255) ggtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGaac > 1:2114402/1‑43 (MQ=255) ggtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGaac > 1:1861980/1‑43 (MQ=255) ggtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGaac > 1:1015473/1‑43 (MQ=255) gtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAg < 1:2534805/69‑1 (MQ=255) gtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAg < 1:1002187/69‑1 (MQ=255) gtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAg < 1:3164691/69‑1 (MQ=255) gtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAg < 1:3413043/69‑1 (MQ=255) gtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAg < 1:588468/69‑1 (MQ=255) gtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAg < 1:939263/69‑1 (MQ=255) gtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAg < 1:2492895/69‑1 (MQ=255) gtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAg < 1:1001932/69‑1 (MQ=255) gtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAg < 1:2467274/69‑1 (MQ=255) gtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAg < 1:2437629/69‑1 (MQ=255) gtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAg < 1:1300017/69‑1 (MQ=255) gtggCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAg < 1:1341210/69‑1 (MQ=255) gtcgcgcTCGGTTGTAAATATCTGCGAATTTGCCACCTGAACAACTGCGCCACGGGTTTAGCAACTCAg < 1:32498/66‑1 (MQ=255) gtcgcgcTCGGTTGTAAATATCTGCGAATTTGCCACCTGAACAACTGCGCCACGGGTGTAGCAACTCAg < 1:1527513/66‑1 (MQ=255) gtcgcgcTCGGTTGTAAATATCTGCGAATTTGCCACCTGAACAACTGCGCCACGGGTGTAGCAACTCAg < 1:1636271/66‑1 (MQ=255) gtcgcgcTCGGTTGTAAATATCTGCGAATTTGCCACCTGAACAACTGCGCCACGGGTGTAGCAACTCAg < 1:3375473/66‑1 (MQ=255) gtcgcgcTCGGTTGTAAATATCTGCGAATTTGCCACCTGAACAACTGCGCCACGGGTGTAGCAACTCAg < 1:3349278/66‑1 (MQ=255) gtcgcgcTCGGTTGTAAATATCTGCGAATTTGCCACCTGAACAACTGCGCCACGGGTGTAGCAACTCAg < 1:2184738/66‑1 (MQ=255) gtcgcgcTCGGTTGTAAATATCTGCGAATTTGCCACCTGAACAACTGCGCCACGGGTGTAGCAACTCAg < 1:322535/66‑1 (MQ=255) gtcgcgcTCGGTTGTAAATATCTGCGAATTTGCCACCTGAACAACTGCGCCACGGGTGTAGCAACTCAg < 1:3203909/66‑1 (MQ=255) gtcgcgcTCGGTTGTAAATATCTGCGAATTTGCCACCTGAACAACTGCGCCACGGGTGTAGCAACTCAg < 1:236592/66‑1 (MQ=255) gtcgcgcTCGGTTGTAAATATCTGCGAATTTGCCACCTGAACAACTGCGCCACGGGTGTAGCAACTCAg < 1:1291897/66‑1 (MQ=255) gtcgcgcTCGGTTGTAAATATCTGCGAATTTGCCACCTGAACAACTGCGCCACGGGTGTAGCAACTCAg < 1:772632/66‑1 (MQ=255) gtcgcgcTCGGTTGTAAATATCTGCGAATTTGCCACCTGAACAACTGCGCCACGGGTGTAGCAACTCAg < 1:776663/66‑1 (MQ=255) gcgcTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGTGTGTAGCAACt < 1:417895/63‑1 (MQ=255) gcgcTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACt < 1:2495295/63‑1 (MQ=255) gcgcTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACt < 1:3063492/63‑1 (MQ=255) gcgcTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACt < 1:2919517/63‑1 (MQ=255) gcgcTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACt < 1:2487055/63‑1 (MQ=255) cGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGc > 1:3033403/1‑54 (MQ=255) cGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAGGATGACaa > 1:2194342/1‑69 (MQ=255) cGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAGGATGACaa > 1:541354/1‑69 (MQ=255) cGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAGGATGACaa > 1:793284/1‑69 (MQ=255) cGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAGGATGACaa > 1:797120/1‑69 (MQ=255) cGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAGGATGACaa > 1:1265113/1‑69 (MQ=255) cTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAGGATGACAAAc < 1:2800633/68‑1 (MQ=255) cTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAGGATGACAAAc < 1:975548/68‑1 (MQ=255) tACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAGGATGACATACTGCGTAAg > 1:3150582/1‑65 (MQ=255) tACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAGGATGACAAACTGCGTAAg > 1:472767/1‑65 (MQ=255) tACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAGGATGACAAACTGCGTAAg > 1:984375/1‑65 (MQ=255) | CGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCAGGATGACAAACTGCGTAAG > minE/2183075‑2183195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |