| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 189,568 | G→A | 24.8% | A355A (GCG→GCA) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 189,568 | 0 | G | A | 24.8% | 134.9 / 37.4 | 108 | A355A (GCG→GCA) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (41/40); new base A (15/12); total (56/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-01 | |||||||||||
Only 100 of 109 total aligned reads displayed.
CACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTG > minE/189511‑189634 | cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCgg > 1:2208962/1‑59 (MQ=255)cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:3321566/1‑69 (MQ=255)cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:2464134/1‑69 (MQ=255)cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:2468456/1‑69 (MQ=255)cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:2573134/1‑69 (MQ=255)cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:2631389/1‑69 (MQ=255)cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:2939980/1‑69 (MQ=255)cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:3065936/1‑69 (MQ=255)cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:3241961/1‑69 (MQ=255)cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:3263124/1‑69 (MQ=255)cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:364129/1‑69 (MQ=255)cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:54795/1‑69 (MQ=255)cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:624837/1‑69 (MQ=255)cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:1332358/1‑69 (MQ=255)cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:869181/1‑69 (MQ=255)cacCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:1394297/1‑69 (MQ=255)cacCCATTTTCATCCGATCTAACAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:1086996/1‑69 (MQ=255)cacCCATTTTCATCCGATATACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGAt > 1:834374/1‑69 (MQ=255) catttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCg < 1:2063078/67‑1 (MQ=255) aTTTTCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCg < 1:1658421/69‑1 (MQ=255) aTTTTCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCg < 1:2494850/69‑1 (MQ=255) aTTTTCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCg < 1:3198025/69‑1 (MQ=255) aTTTTCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCg < 1:2784503/69‑1 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:776662/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:940438/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:253841/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:882276/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:260693/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:868209/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:2996070/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:3147853/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:72894/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:1081765/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:336837/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:639226/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:62321/1‑69 (MQ=255) ttttCATCCAATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCAGGAAAAGTGATCTTCGa > 1:1004670/1‑69 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTg < 1:83950/59‑1 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTg < 1:1581250/59‑1 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTg < 1:422737/59‑1 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTg < 1:417747/59‑1 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTg < 1:3281932/59‑1 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTg < 1:2678313/59‑1 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGaaa > 1:23135/1‑69 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGaaa > 1:3016047/1‑69 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTCCGGGAAAAGTg < 1:118681/59‑1 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGAGAGCGTGTTGCGGGAAAAGTg < 1:3278756/59‑1 (MQ=255) tCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGaaa > 1:3144112/1‑46 (MQ=255) tCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGaaa > 1:553503/1‑46 (MQ=255) tCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGaaa > 1:1785030/1‑46 (MQ=255) tCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGaaa > 1:232895/1‑46 (MQ=255) aGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCa < 1:242336/58‑1 (MQ=255) aGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCa < 1:2602170/58‑1 (MQ=255) aGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCa < 1:1089521/58‑1 (MQ=255) aGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCa < 1:2069847/58‑1 (MQ=255) aGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCcac > 1:2950196/1‑68 (MQ=255) gggTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaa < 1:1206077/69‑1 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:1662716/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:712502/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:2315135/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:2556654/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:1796988/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:1970603/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:585194/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:1249848/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:2711653/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaa > 1:2103265/1‑68 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc < 1:2115927/48‑1 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc < 1:3193605/48‑1 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc < 1:2933852/48‑1 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc < 1:834198/48‑1 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc < 1:2366415/48‑1 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc < 1:1464669/48‑1 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc < 1:701176/48‑1 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTa > 1:574182/1‑69 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTa > 1:1347004/1‑69 (MQ=255) ggcgAGCGTGTTGCAGGAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCg < 1:1338841/69‑1 (MQ=255) ggcgAGCGTGTTGCAGGAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCg < 1:109310/69‑1 (MQ=255) ggcgAGCGTGTTGCAGGAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2295949/69‑1 (MQ=255) ggcgAGCGTGTTGCAGGAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2221391/69‑1 (MQ=255) ggcgAGCGTGTTGCAGGAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2190002/69‑1 (MQ=255) gcgAGCGTGTTGCAGGAAAAGTGATCTTCGTAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATct < 1:1281597/68‑2 (MQ=255) gcgAGCGTGTTGCAGGAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2710542/68‑1 (MQ=255) gCGGGAAATGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:3239273/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2319434/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:758558/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2335422/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:1767990/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:3306359/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2263803/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2860337/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:214393/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2201670/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2660847/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:951077/69‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:1707793/69‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:3082972/69‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:2872832/69‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:2247072/69‑1 (MQ=255) cGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:1951951/56‑1 (MQ=255) | CACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTG > minE/189511‑189634 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |