| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,183,396 | G→A | 37.1% | P1224P (CCG→CCA) | gltB → | glutamate synthase, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,183,396 | 0 | G | A | 37.1% | 66.8 / 64.5 | 105 | P1224P (CCG→CCA) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (25/41); new base A (10/29); total (35/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
AGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCG > minE/2183329‑2183462 | agCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGc > 1:970612/1‑69 (MQ=255)agCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGc > 1:1493698/1‑69 (MQ=255) ggACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATcc < 1:3114338/69‑1 (MQ=255) ggACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATcc < 1:2043554/69‑1 (MQ=255) ggACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATcc < 1:1887101/69‑1 (MQ=255) ggACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATcc < 1:1396800/69‑1 (MQ=255) gACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATcc < 1:2183287/68‑1 (MQ=255) tttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTaa > 1:1762374/1‑69 (MQ=255) tttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTaa > 1:3252588/1‑69 (MQ=255) tttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTaa > 1:33866/1‑69 (MQ=255) tttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTaa > 1:966723/1‑69 (MQ=255) tttCACCGCCAAACAGCAGAAGCTGGCGC‑‑TCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg < 1:2496415/69‑1 (MQ=255) ttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTaa > 1:1384855/1‑68 (MQ=255) ttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTaa > 1:2125107/1‑68 (MQ=255) ttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTaa > 1:326778/1‑68 (MQ=255) ttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGATGGAGACTGCCGAACCGCATCCAGGTa > 1:1230298/1‑67 (MQ=255) tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg < 1:221899/69‑1 (MQ=255) tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg < 1:2794298/69‑1 (MQ=255) tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg < 1:2875744/69‑1 (MQ=255) tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg < 1:2970278/69‑1 (MQ=255) tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg < 1:3138907/69‑1 (MQ=255) tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg < 1:1786114/69‑1 (MQ=255) tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg < 1:3303937/69‑1 (MQ=255) tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg < 1:1267144/69‑1 (MQ=255) tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg < 1:372566/69‑1 (MQ=255) tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg < 1:383041/69‑1 (MQ=255) ccGCCAAACAGCATAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCATGTAAGGCa < 1:1452186/68‑1 (MQ=255) ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTATGGCa < 1:3156433/68‑1 (MQ=255) ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa < 1:3226835/68‑1 (MQ=255) ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa < 1:6873/68‑1 (MQ=255) ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa < 1:2021297/68‑1 (MQ=255) ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa < 1:278398/68‑1 (MQ=255) ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa < 1:2512773/68‑1 (MQ=255) ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa < 1:3423096/68‑1 (MQ=255) cGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa < 1:424661/67‑1 (MQ=255) gCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCActc < 1:1154114/69‑1 (MQ=255) gCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCActc < 1:2383324/69‑1 (MQ=255) gCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCActc < 1:2884747/69‑1 (MQ=255) gCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaaa > 1:2748602/1‑68 (MQ=255) gCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaaa > 1:1132087/1‑68 (MQ=255) gCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaaa > 1:1395957/1‑68 (MQ=255) gCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaaa > 1:1550754/1‑68 (MQ=255) gCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaa > 1:3257779/1‑67 (MQ=255) ggCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAaacaa < 1:1375244/69‑1 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCActct < 1:914966/44‑1 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:920822/1‑68 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:111614/1‑68 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:393442/1‑68 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:703498/1‑68 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:918324/1‑68 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:1500274/1‑68 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:1735917/1‑68 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:1894726/1‑68 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:2191679/1‑68 (MQ=255) gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGTCACTCTACTGTACCGAAAACAACccgccg < 1:2639378/67‑1 (MQ=255) gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg < 1:2393067/67‑1 (MQ=255) gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg < 1:357386/67‑1 (MQ=255) gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg < 1:330133/67‑1 (MQ=255) gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg < 1:1834479/67‑1 (MQ=255) gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg < 1:2082570/67‑1 (MQ=255) gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg < 1:2941575/67‑1 (MQ=255) aaGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCActct < 1:2112899/43‑1 (MQ=255) ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt < 1:1058424/69‑1 (MQ=255) ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt < 1:1719534/69‑1 (MQ=255) ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt < 1:3082223/69‑1 (MQ=255) ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt < 1:2610036/69‑1 (MQ=255) ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt < 1:580048/69‑1 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAaaca > 1:1240965/1‑52 (MQ=25) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:232890/1‑69 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:514371/1‑69 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:1784893/1‑69 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:261281/1‑69 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:1856091/1‑69 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:1505580/1‑69 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:1395242/1‑69 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:1345464/1‑69 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:225756/1‑69 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATACCGGc < 1:3412125/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:351995/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:589507/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:2464838/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:1206617/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:49821/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:460401/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:407125/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:1679624/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:2360141/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:1720395/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:1831284/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:3232499/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:2780988/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:1924626/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:3146145/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:1993824/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:2043713/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:3009857/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:2160520/65‑1 (MQ=255) ttcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:790923/65‑1 (MQ=255) ccGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAcgcg < 1:1675732/69‑1 (MQ=255) ccGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAcgcg < 1:1563192/69‑1 (MQ=255) | AGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCG > minE/2183329‑2183462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |