Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,183,400 C→G 40.2% P1226A (CCA→GCA)  gltB → glutamate synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,183,4000CG40.2% 53.7 / 94.8 97P1226A (CCA→GCA) gltBglutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base C (23/35);  new base G (10/29);  total (33/64)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 103 total aligned reads displayed.

GGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCG  >  minE/2183333‑2183462
                                                                   |                                                              
ggACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATcc                                                               <  1:211762/69‑1 (MQ=255)
ggACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATcc                                                               <  1:2043554/69‑1 (MQ=255)
ggACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATcc                                                               <  1:1887101/69‑1 (MQ=255)
ggACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATcc                                                               <  1:1396800/69‑1 (MQ=255)
 gACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATcc                                                               <  1:2183287/68‑1 (MQ=255)
      tttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTaa                                                         >  1:966723/1‑69 (MQ=255)
      tttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTaa                                                         >  1:3252588/1‑69 (MQ=255)
      tttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTaa                                                         >  1:1762374/1‑69 (MQ=255)
      tttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTaa                                                         >  1:33866/1‑69 (MQ=255)
      tttCACCGCCAAACAGCAGAAGCTGGCGC‑‑TCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg                                                       <  1:2496415/69‑1 (MQ=255)
       ttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTaa                                                         >  1:1384855/1‑68 (MQ=255)
       ttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTaa                                                         >  1:2125107/1‑68 (MQ=255)
       ttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTaa                                                         >  1:326778/1‑68 (MQ=255)
       ttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTaa                                                         >  1:347670/1‑68 (MQ=255)
       ttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGATGGAGACTGCCGAACCGCATCCAGGTa                                                          >  1:1230298/1‑67 (MQ=255)
        tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg                                                       <  1:3138907/69‑1 (MQ=255)
        tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg                                                       <  1:1786114/69‑1 (MQ=255)
        tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg                                                       <  1:221899/69‑1 (MQ=255)
        tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg                                                       <  1:1646759/69‑1 (MQ=255)
        tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg                                                       <  1:383041/69‑1 (MQ=255)
        tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg                                                       <  1:372566/69‑1 (MQ=255)
        tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg                                                       <  1:2794298/69‑1 (MQ=255)
        tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg                                                       <  1:2875744/69‑1 (MQ=255)
        tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg                                                       <  1:1267144/69‑1 (MQ=255)
        tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg                                                       <  1:2970278/69‑1 (MQ=255)
        tCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg                                                       <  1:3303937/69‑1 (MQ=255)
           ccGCCAAACAGCATAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCATGTAAGGCa                                                     <  1:1452186/68‑1 (MQ=255)
           ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTATGGCa                                                     <  1:3156433/68‑1 (MQ=255)
           ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa                                                     <  1:278398/68‑1 (MQ=255)
           ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa                                                     <  1:2512773/68‑1 (MQ=255)
           ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa                                                     <  1:2021297/68‑1 (MQ=255)
           ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa                                                     <  1:6873/68‑1 (MQ=255)
           ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa                                                     <  1:3226835/68‑1 (MQ=255)
           ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa                                                     <  1:3423096/68‑1 (MQ=255)
            cGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa                                                     <  1:424661/67‑1 (MQ=255)
             gCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCActc                                                  <  1:1154114/69‑1 (MQ=255)
             gCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCActc                                                  <  1:2383324/69‑1 (MQ=255)
             gCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCActc                                                  <  1:2884747/69‑1 (MQ=255)
                           gCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaaa                                     >  1:1395957/1‑68 (MQ=255)
                           gCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaaa                                     >  1:1132087/1‑68 (MQ=255)
                           gCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaaa                                     >  1:2748602/1‑68 (MQ=255)
                           gCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaaa                                     >  1:1550754/1‑68 (MQ=255)
                           gCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaa                                      >  1:3257779/1‑67 (MQ=255)
                              ggCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAaacaa                                 <  1:1375244/69‑1 (MQ=255)
                                       gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCActct                                                 <  1:914966/44‑1 (MQ=255)
                                       gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt                         >  1:393442/1‑68 (MQ=255)
                                       gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt                         >  1:111614/1‑68 (MQ=255)
                                       gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt                         >  1:703498/1‑68 (MQ=255)
                                       gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt                         >  1:918324/1‑68 (MQ=255)
                                       gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt                         >  1:920822/1‑68 (MQ=255)
                                       gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt                         >  1:1500274/1‑68 (MQ=255)
                                       gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt                         >  1:1735917/1‑68 (MQ=255)
                                       gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt                         >  1:1894726/1‑68 (MQ=255)
                                       gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt                         >  1:2191679/1‑68 (MQ=255)
                                       gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGTCACTCTACTGTACCGAAAACAACccgccg                          <  1:2639378/67‑1 (MQ=255)
                                       gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg                          <  1:2393067/67‑1 (MQ=255)
                                       gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg                          <  1:357386/67‑1 (MQ=255)
                                       gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg                          <  1:330133/67‑1 (MQ=255)
                                       gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg                          <  1:1834479/67‑1 (MQ=255)
                                       gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg                          <  1:2082570/67‑1 (MQ=255)
                                       gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg                          <  1:2941575/67‑1 (MQ=255)
                                        aaGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCActct                                                 <  1:2112899/43‑1 (MQ=255)
                                           ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt                    <  1:1058424/69‑1 (MQ=255)
                                           ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt                    <  1:580048/69‑1 (MQ=255)
                                           ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt                    <  1:1719534/69‑1 (MQ=255)
                                           ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt                    <  1:2610036/69‑1 (MQ=255)
                                           ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt                    <  1:3082223/69‑1 (MQ=255)
                                              ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAaaca                                  >  1:1240965/1‑52 (MQ=25)
                                              ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc                 >  1:1784893/1‑69 (MQ=255)
                                              ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc                 >  1:1505580/1‑69 (MQ=255)
                                              ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc                 >  1:514371/1‑69 (MQ=255)
                                              ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc                 >  1:225756/1‑69 (MQ=255)
                                              ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc                 >  1:261281/1‑69 (MQ=255)
                                              ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc                 >  1:232890/1‑69 (MQ=255)
                                              ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc                 >  1:1856091/1‑69 (MQ=255)
                                              ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc                 >  1:1395242/1‑69 (MQ=255)
                                              ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc                 >  1:1345464/1‑69 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATACCGGc              <  1:3412125/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:2464838/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:1679624/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:589507/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:2843336/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:49821/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:1206617/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:460401/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:2160520/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:407125/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:2360141/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:351995/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:1720395/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:3009857/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:1831284/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:3232499/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:2780988/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:1924626/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:3146145/65‑1 (MQ=255)
                                                  attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:2043713/65‑1 (MQ=255)
                                                   ttcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc              <  1:790923/65‑1 (MQ=255)
                                                             ccGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAcgcg  <  1:1563192/69‑1 (MQ=255)
                                                             ccGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAcgcg  <  1:1675732/69‑1 (MQ=255)
                                                                   |                                                              
GGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCG  >  minE/2183333‑2183462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: