| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,183,420 | C→T | 50.6% | C1232C (TGC→TGT) | gltB → | glutamate synthase, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,183,420 | 0 | C | T | 50.6% | ‑5.0 / 71.9 | 77 | C1232C (TGC→TGT) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base C (29/9); new base T (10/29); total (39/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01 | |||||||||||
GCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGT > minE/2183360‑2183487 | gCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaaa > 1:1132087/1‑68 (MQ=255)gCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaaa > 1:2748602/1‑68 (MQ=255)gCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaaa > 1:1550754/1‑68 (MQ=255)gCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaaa > 1:1395957/1‑68 (MQ=255)gCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaa > 1:3257779/1‑67 (MQ=255) ggCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAaacaa < 1:1375244/69‑1 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:111614/1‑68 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:1894726/1‑68 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:1735917/1‑68 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:2191679/1‑68 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:1500274/1‑68 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:393442/1‑68 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:703498/1‑68 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:918324/1‑68 (MQ=255) gAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt > 1:920822/1‑68 (MQ=255) gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGTCACTCTACTGTACCGAAAACAACccgccg < 1:2639378/67‑1 (MQ=255) gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg < 1:330133/67‑1 (MQ=255) gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg < 1:357386/67‑1 (MQ=255) gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg < 1:2941575/67‑1 (MQ=255) gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg < 1:2082570/67‑1 (MQ=255) gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg < 1:2393067/67‑1 (MQ=255) gAAGCTGCTGGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACccgccg < 1:1834479/67‑1 (MQ=255) ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt < 1:982952/69‑1 (MQ=255) ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt < 1:3082223/69‑1 (MQ=255) ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt < 1:580048/69‑1 (MQ=255) ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt < 1:2610036/69‑1 (MQ=255) ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt < 1:1058424/69‑1 (MQ=255) ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt < 1:1719534/69‑1 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAaaca > 1:1240965/1‑52 (MQ=25) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:225756/1‑69 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:261281/1‑69 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:1505580/1‑69 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:1395242/1‑69 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:232890/1‑69 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:1345464/1‑69 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:514371/1‑69 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:1784893/1‑69 (MQ=255) ctgGAAACTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAAc > 1:1856091/1‑69 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATACCGGc < 1:3412125/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:351995/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:2780988/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:407125/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:460401/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:49821/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:3232499/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:1206617/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:3146145/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:589507/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:3009857/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:1924626/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:1993824/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:2043713/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:1679624/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:1720395/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:2464838/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:2360141/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:2843336/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:2160520/65‑1 (MQ=255) attcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:1831284/65‑1 (MQ=255) ttcTGCCGAGCCACATGCAGGTAAGGCACTCTACTGTACCGAAAACAACCCGCCGTTTGATAACGGc < 1:790923/65‑1 (MQ=255) ccGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAcgcg < 1:1563192/69‑1 (MQ=255) ccGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAcgcg < 1:1675732/69‑1 (MQ=255) aaGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtgctgc > 1:2227963/1‑67 (MQ=255) ctACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTACTGAACGCGCAGTTGCTGCAACAGGCGaa > 1:2690741/1‑69 (MQ=255) aCTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAAc > 1:2252992/1‑69 (MQ=255) aCTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAAc > 1:2480672/1‑69 (MQ=255) aCTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAAc > 1:2799157/1‑69 (MQ=255) aCTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAAc > 1:1295367/1‑69 (MQ=255) gCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCg > 1:364182/1‑68 (MQ=255) gCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCg > 1:1890099/1‑68 (MQ=255) gCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGt > 1:998330/1‑69 (MQ=255) gCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGt > 1:72274/1‑69 (MQ=255) gCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGt > 1:445664/1‑69 (MQ=255) gCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGt > 1:1316772/1‑69 (MQ=255) gCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGt > 1:3202338/1‑69 (MQ=255) gCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGt > 1:2870363/1‑69 (MQ=255) gCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGt > 1:2203469/1‑69 (MQ=255) | GCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGT > minE/2183360‑2183487 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |