| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,183,975 | T→C | 22.3% | G1417G (GGT→GGC) | gltB → | glutamate synthase, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,183,975 | 0 | T | C | 22.3% | 74.1 / 15.9 | 58 | G1417G (GGT→GGC) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base T (41/4); new base C (9/4); total (50/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.60e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGGTTGTGAATATATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAA > minE/2183910‑2184040 | taCGGTTGTGAAGATATGACGGGTGGTATCGTCTGCATTCTCGGTAAAACCGGCGTTAACTTCGGTGc > 1:2373891/2‑68 (MQ=255)aaCGGTTGTGAATATATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCg > 1:2607993/1‑69 (MQ=255)aaCGGTTGTGAATATATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCg > 1:1198211/1‑69 (MQ=255)aaCGGTTGTGAATATATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCg > 1:2236468/1‑69 (MQ=255) aCGGTTGTGAATATATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCg > 1:724972/1‑68 (MQ=255) aCGGTTGTGAATATATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCg > 1:3002235/1‑68 (MQ=255) aCGGTTGTGAATATATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCg > 1:2032365/1‑68 (MQ=255) aCGGTTGTGAATATATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCg > 1:1599739/1‑68 (MQ=255) ggTTGTGAATATATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGc < 1:981496/69‑1 (MQ=255) tgtgAATATATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATg > 1:1322097/1‑69 (MQ=255) gtggtATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCtt > 1:1462166/1‑69 (MQ=255) tggtATCGTCTGCATTCTGGGTAAAAGCGGCGTTAACTACGGTGCGGGCATGACCGGCGGTTTCGCTTa > 1:2004502/1‑69 (MQ=255) tggtATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTa > 1:668938/1‑69 (MQ=255) tCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGtt > 1:1377704/1‑68 (MQ=255) tCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtc > 1:1116383/1‑69 (MQ=255) tCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtc > 1:539861/1‑69 (MQ=255) tCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtc > 1:18809/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:2559771/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:2454631/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:2713262/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:3277690/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:408952/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:1950041/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:1093396/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:151409/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGAGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:1594196/1‑69 (MQ=255) tGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGAt < 1:63892/69‑1 (MQ=255) tGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGAt < 1:11433/69‑1 (MQ=255) cTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAgc > 1:528176/1‑69 (MQ=255) cTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAgc > 1:812544/1‑69 (MQ=255) cTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAgc > 1:1389896/1‑69 (MQ=255) cTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAg > 1:2502503/1‑68 (MQ=255) cTGGGTAAAACCGGCGTTAACTTCGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAgc < 1:786047/69‑1 (MQ=255) cTGGGTAAAACCGGCGTTAACTTCGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAgc < 1:3138120/69‑1 (MQ=255) cTGGGTAAAACCGGCGTTAACTTCGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAgc < 1:1082862/69‑1 (MQ=255) tGGGTAAAACCGGCGTTAACTTCGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAgc < 1:1411166/68‑1 (MQ=255) aaaaCCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGAt > 1:1711929/1‑69 (MQ=255) aaaaCCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGAt > 1:1976468/1‑69 (MQ=255) tAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcg < 1:1573930/69‑1 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAgc > 1:2113963/1‑51 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcgc > 1:467835/1‑69 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcgc > 1:944283/1‑69 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcgc > 1:93903/1‑69 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcgc > 1:2153543/1‑69 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcgc > 1:2252162/1‑69 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcgc > 1:2289564/1‑69 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcgc > 1:3127318/1‑69 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcgc > 1:2625508/1‑69 (MQ=255) aaCTTCGGTGCGGGCATCACCGGCGGTTTCGCTTACGtt > 1:3176537/1‑39 (MQ=255) cGGCGCGGGCATGACCTGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:175930/1‑69 (MQ=255) cGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:3424001/1‑69 (MQ=255) cGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:1262471/1‑69 (MQ=255) cGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:2457583/1‑69 (MQ=255) cGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:3188512/1‑69 (MQ=255) cGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:1903137/1‑69 (MQ=255) cGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:681821/1‑69 (MQ=255) cGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:1192096/1‑69 (MQ=255) cGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:241118/1‑69 (MQ=255) | AACGGTTGTGAATATATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAA > minE/2183910‑2184040 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |